Multiple antigenic peptide-based flow through dot-blot assay for simultaneous antibody detection of infectious bronchitis virus and Newcastle disease virus

Tomar, Piyush; Joshi, Vinay G.; Mahajan, N. K.; Jindal, Naresh

doi:10.1016/j.biologicals.2021.07.005

Cited by 1 publication

(1 citation statement)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Rapid testing is the primary tool for clinically monitoring viral infections and developing appropriate treatments. Against the most prevalent viral pathogens circulating in goose, like Goose parvovirus (GPV), Newcastle disease virus (NDV), goose herpesvirus (GHV), and goose adenovirus (GAV), several molecular assays have been developed for clinical diagnosis in the past few years by using either genomic detection methods such as PCR or antigen detection methods such as ELISA ( Chen et al, 2009 ; Guo et al, 2009 ; Wan et al, 2019 ; Wu et al, 2019 ; Tomar et al, 2021 ). Also, several duplex detection assays were developed for rapid screening of multiple pathogens, usually by duplex PCR ( Kong et al, 2007 ; Chen et al, 2013 ; Wang et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Rapid diagnosis of duck Tembusu virus and goose astrovirus with TaqMan-based duplex real-time PCR

Wan

Wang³

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

The mixed infection of duck Tembusu virus (DTMUV) and goose astrovirus (GoAstV) is an important problem that endangers the goose industry. Although quantitative PCR has been widely used in monitoring these two viruses, there is no reliable method to detect them at the same time. In this study, by analyzing the published genomes of DTMUV and goose astrovirus genotype 2 (GoAstV-2) isolated in China, we found that both viruses have high conservation, showing 96.5 to 99.5% identities within different strains of DTMUV and GoAstV, respectively. Subsequently, PCR primers and TaqMan probes were designed to identify DTMUV and GoAstV-2, and different fluorescent reporters were given to two probes for differential diagnosis. Through the optimization and verification, this study finally developed a duplex TaqMan qPCR method that can simultaneously detect the above two viruses. The lower limits of detection were 100 copies/μL and 10 copies/μL for DTMUV and GoAstV-2 under optimal condition. The assay was also highly specific in detecting one or two viruses in various combinations in specimens, and provide tool for clinical diagnosis of mixed infections of viruses in goose.

show abstract