Multiple bacterial species reside in chronic wounds: a longitudinal study

Gjødsbøl, Kristine; Christensen, Jens Jørgen; Karlsmark, Tonny; Jørgensen, Bo; Klein, Bjarke M.; Krogfelt, Karen A.

doi:10.1111/j.1742-481x.2006.00159.x

Cited by 560 publications

(560 citation statements)

References 15 publications

(25 reference statements)

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“…This is in consistent with various reports that S. aureus and P. aeruginosa were among the most common bacteria isolated from wounds of different aetiologies [13,30,31]. In this study, higher occurrence rates of S. aureus and P. aeruginosa were observed in chronic wounds than in acute wounds.…”

Section: Discussionsupporting

confidence: 82%

Prevalence and antibiotic susceptibility of bacteria from acute and chronic wounds in Malaysian subjects

Wong

Manikam

Muniandy

2015

J Infect Dev Ctries

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Introduction: Chronic wounds represent a major health burden worldwide. It has been hypothesized that the polymicrobial nature of wounds plays an important role in their healing process. Thus, a review of pathogen frequency and susceptibility patterns in wounds is necessary to provide appropriate guidelines for antimicrobial usage. Methodology: In this study, microbiota and antimicrobial resistance in both acute and chronic wound patients treated at the University of Malaya Medical Centre, Malaysia, were compared. Wound swabs from 84 patients with acute wounds and 84 patients with chronic wounds were collected. The specimens were cultured using standard microbiological techniques. Isolates were then tested for antibiotic sensitivity with the broth microdilution method. Results: Of 210 pathogenic bacteria isolates, Staphylococcus aureus (49; 23.3%) and Pseudomonas aeruginosa (31; 14.8%) were the most prevalent bacteria found in wounds. Staphylococcus aureus was found significantly more often in patients with chronic wounds (41; 48.8%) than in patients with acute wounds (8; 9.5%), while Staphylococcus epidermidis was found predominantly in acute wounds (15; 17.9%). At the time of study, patients with chronic wounds (58.3%) had received more antibiotic treatments in the past previous 12 months compared with patients with acute wounds (16.7%). In the antibiotic susceptibility test, Staphylococcus spp. revealed highest resistance towards penicillin and ampicillin. Isolates showed no decrease in susceptibility against a number of newly developed antibiotics (linezolid, daptomycin, and tigecycline). Conclusions: Our finding showed that bacteria diversity and antimicrobial-resistant strains are more frequently found in chronic wounds than in acute wounds.

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Section: Discussionsupporting

confidence: 82%

Prevalence and antibiotic susceptibility of bacteria from acute and chronic wounds in Malaysian subjects

Wong

Manikam

Muniandy

2015

J Infect Dev Ctries

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“…[13][14][15] Several reports have linked biofilms to the induction and persistence of inflammation and delayed healing in wound infections. 1,9,[15][16][17][18][19][20][21] In addition, studies (using a combination of traditional culture methods, microscopic analyses, and molecular techniques) involving wound samples from human patients support the presence of mixed populations of microorganisms in different types of chronic wounds. 9,11,13,18,19,22,23 However, despite several studies linking biofilm infection to delayed wound healing, the mechanisms and significance of biofilms in wound infection remain poorly understood.…”

Section: Models Of Biofilm Infectionsmentioning

confidence: 99%

Chronic Wound Biofilm Model

Ganesh

Sinha

Mathew-Steiner

et al. 2015

Advances in Wound Care

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“…It is also the predominant cause of chronic lung infection in cystic fibrosis patients (Frederiksen et al, 1997) and has recently been recognized as one of the main causes of chronic wound infections (Gjødsbøl et al, 2006). P. aeruginosa can infect patients by producing a wide range of virulence factors, the expression levels of which are tightly regulated.…”

Section: Introductionmentioning

confidence: 99%

Screening for novel quorum-sensing inhibitors to interfere with the formation of Pseudomonas aeruginosa biofilm

Ding

Yin

et al. 2011

Journal of Medical Microbiology

126

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The objective of this study was to screen for novel quorum-sensing inhibitors (QSIs) from traditional Chinese medicines (TCMs) that inhibit bacterial biofilm formation. Six of 46 active components found in TCMs were identified as putative QSIs based on molecular docking studies. Of these, three compounds inhibited biofilm formation by Pseudomonas aeruginosa and Stenotrophomonas maltophilia at a concentration of 200 mM. A fourth compound (emodin) significantly inhibited biofilm formation at 20 mM and induced proteolysis of the quorum-sensing signal receptor TraR in Escherichia coli at a concentration of 3-30 mM. Emodin also increased the activity of ampicillin against P. aeruginosa. Therefore, emodin might be suitable for development into an antivirulence and antibacterial agent. INTRODUCTIONPseudomonas aeruginosa, an opportunistic human pathogen, may cause acute infections in hospitalized patients, can be isolated from the environment, particularly from soil and water, and it regularly contaminates medical devices (Stover et al., 2000). It is also the predominant cause of chronic lung infection in cystic fibrosis patients (Frederiksen et al., 1997) and has recently been recognized as one of the main causes of chronic wound infections (Gjødsbøl et al., 2006). P. aeruginosa can infect patients by producing a wide range of virulence factors, the expression levels of which are tightly regulated. Key to this regulation is cell density-dependent cell-to-cell signalling, which is termed quorum sensing (QS) (Rumbaugh et al., 2000). As in many other bacteria, QS controls secretion of virulence factors (Mittal et al., 2006), biofilm formation (Waters et al., 2008) and the exchange of DNA (Fuqua & Winans, 1996) in P. aeruginosa. The biofilm mode of growth is recognized as an important bacterial trait that is relevant to infections (Costerton et al., 1994). Many infections involve the formation of bacterial biofilms, which are bacterial communities that settle and proliferate on surfaces and are covered by exopolymers (Lewis, 2007). Once established, biofilms are difficult to eradicate and become a source of secondary infection (Jones et al., 2009). Moreover, bacteria embedded in biofilms are more tolerant than planktonic cells of antibiotics (Donlan & Costerton, 2002;Drenkard, 2003). The dose of antibiotics needed in this situation will often exceed the highest deliverable dose, which makes efficient treatment impossible.QS, as a regulatory mechanism, enables bacteria to make collective decisions with respect to the expression of a specific set of genes that involve the production, release and subsequent detection of chemical signalling molecules, such as N-acylhomoserine lactones (AHLs) that are commonly used by Gram-negative bacteria. When the concentration of AHLs reaches a certain threshold level, binding to a receptor molecule (for example, LuxR) is promoted and the activated LuxR-AHL complex forms dimers or polymers, which, in turn, act as transcriptional regulators of target genes in the QS regulon (Parsek & Greenbe...

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Multiple bacterial species reside in chronic wounds: a longitudinal study

Cited by 560 publications

References 15 publications

Prevalence and antibiotic susceptibility of bacteria from acute and chronic wounds in Malaysian subjects

Prevalence and antibiotic susceptibility of bacteria from acute and chronic wounds in Malaysian subjects

Chronic Wound Biofilm Model

Screening for novel quorum-sensing inhibitors to interfere with the formation of Pseudomonas aeruginosa biofilm

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