Multiple cases of acquired toxoplasmosis retinitis presenting in an outbreak

Burnett, Andrew J; Shortt, Stanley G; Isaac-Renton, Judith L.; King, Arlene; Werker, Denise; Bowie, William

doi:10.1016/s0161-6420(98)96004-3

Cited by 205 publications

(98 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…When bands are intense, such as in Fig. 1, PCR products have to be diluted 1/15 in deionized formamid before electrophoresis in an automatic 10 (TG) 2 (1) (TG) 9 (1) (CA) 10 (1.3) (TC) 10 (1) 291 (1) 248 (1.6) 204 (1) (1) (TC) 9 (TG) 3 (6) (TG) 7 (3) (CA) 10 (1.3) (TC) 10 (1) 291 (1) 248 (1.6) 200 (3) Atypical (CA) 8 (1) (TC) 6 TT(TG) 2 (7) (TG) 6 (4) (CA) 10 (1.3) (TC) 3 TT(TC) 3 (11) 291 (1) 242 (2.3.7) 198 (4) 158 (1.3) 337 (11) a GenBank accession numbers and MS types are described in reference 2. *, one of the five alleles defining this MS type was an atypical allele not found in the classical type (as described in reference 2).…”

mentioning

confidence: 99%

“…For instance, the 242-bp length at W35 by multiplex PCR corresponds to three different sequences: (TC) 7 (TG) 2 , which is allele 2, (TC) 6 (TG) 3 , which is allele 3, and (TC) 6 TT (TG) 2 , which is an atypical allele 7. Similarly, the 248-bp length at W35 corresponds either to (TC) 10 (TG) 2 , which is allele 1, or to (TC) 9 (TG) 3 , which is another atypical allele (allele 6). However, the association with the four other MS markers allows us to distinguish type I, II, or III or an atypical genotype.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Multiplex PCR for Typing Strains of Toxoplasma gondii

2005

View full text Add to dashboard Cite

show abstract

mentioning

confidence: 99%

mentioning

confidence: 99%

Multiplex PCR for Typing Strains of Toxoplasma gondii

2005

View full text Add to dashboard Cite

show abstract

“…Ocular toxoplasmosis is a potential complication of both acquired acute (6,23,24) and reactivated congenital (29) toxoplasmosis. In France, systematic screening for seroconversion during pregnancy and aggressive antenatal and postnatal treatment policy for affected children has led to a marked decline in the incidence of severe congenital toxoplasmosis.…”

mentioning

confidence: 99%

Comparison of Enzyme-Linked Immunosorbent Assay, Immunoblotting, and PCR for Diagnosis of Toxoplasmic Chorioretinitis

et al. 2003

View full text Add to dashboard Cite

Ocular toxoplasmosis is the major cause of posterior uvetis in European populations. The clinical diagnosis of toxoplasmic chorioretinitis is based upon ophthalmoscopic findings, which are often but not always typical. Laboratory testing is therefore important to confirm the etiology of the disease. In the present 2-year prospective study, the relative diagnostic sensitivities of the three analytical techniques (enzyme-linked immunosorbent assay [ELISA], immunoblotting, and PCR) were compared by using a group of patients (n ‫؍‬ 19) with suspected ocular toxoplasmosis. The relative specificities of the three techniques were assessed by including two control groups of patients: one with nontoxoplasmic and noninflammatory ocular disease (n ‫؍‬ 48) and the other with nontoxoplasmic and inflammatory ocular disease (n ‫؍‬ 20). All 19 of the clinically suspect patients had serological evidence of exposure to Toxoplasma gondii: 17 had been previously infected, and 2 had current infection. The analysis of paired aqueous humor and serum samples by ELISA and immunoblotting revealed the local production of specific antibodies of the immunoglobulin G type in 63% (12 of 19) and 53% (10 of 19) of patients, respectively. PCR analysis of aqueous humor samples confirmed the presence of T. gondii DNA in 28% (5 of 18) of cases. When combined, ELISA, immunoblotting, and PCR findings confirmed the toxoplasmic origin of retinal lesions in 83% (15 of 18) of patients. The relative specificities of the three techniques were 89% for ELISA and immunoblotting and 100% for PCR.

show abstract

“…Genotypes of T. gondii strains isolated from Brazil were highly divergent when compared (by multilocus nested PCR analysis combined with sequencing of a polymorphic intron) to the previously described clonal lineages found in Europe. These atypical genotypes may also explain the high frequency (20% of 97 cases) of ocular involvement in the above mentioned Canadian outbreak where an atypical cougar isolate was suspected, and the 100-fold higher incidence of OT in patients born in Africa compared to patients born in Britain [31,32]. The distribution of genotypes was different in immunocompromised patients who reactivate a type II strain (if acquired in Europe), or a non-type II strain (if acquired in Africa or South America).…”

Section: Specific Parasitic Genotypes Could Be Involvedmentioning

confidence: 98%