Multiple Caspases Mediate Acute Renal Cell Apoptosis Induced by Bacterial Cell Wall Components

Langford, Marlyn P.; McGee, David J.; Ta, Kathy H.; Redens, Thomas B.; Texada, Donald E.

doi:10.3109/0886022x.2011.553304

Cited by 12 publications

(9 citation statements)

References 43 publications

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“…We also found that LPS decreased mitochondrial membrane potential, promoted activations of caspase-3 and caspase-9, increased expressions of Bax and cleaved caspase-3 and decreased level of Bcl-2 protein in RAW 264.7 cells, suggesting that LPS likely induced apoptosis through caspase-dependent mitochondrial death pathway. This result is in agreement with results already obtained by Langford et al [40]. However, koumine pretreatment reversed this effect.…”

Section: Discussionsupporting

confidence: 94%

A Potential Mechanism for the Anti-Apoptotic Property of Koumine Involving Mitochondrial Pathway in LPS-Mediated RAW 264.7 Macrophages

Yuan

Liang

et al. 2016

Molecules

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Abstract:Koumine is a kind of alkaloid extracted from Gelsemium elegans (G. elegans). Benth, which has shown promise as an anti-tumor, anxiolytic, and analgesic agent. In our present study, the effect of koumine on lipopolysaccharide (LPS)-mediated RAW 264.7 cell apoptosis was evaluated. MTT assays showed that koumine obviously increased cell viability in LPS-mediated RAW 264.7 macrophages. Preincubation with koumine ameliorated LPS-medicated apoptosis by decreasing reactive oxygen species (ROS) production, which resulted in a significant decrease in the levels of nitric oxide (NO) and inducible nitric oxide synthase (iNOS). In addition, koumine-pretreated RAW 264.7 macrophages exhibited reduction of LPS-induced levels of TNF-α, IL-1β, and IL-6 mRNA. Furthermore, pretreatment with koumine suppressed LPS-mediated p53 activation, loss of mitochondrial membrane potential, caspase-3 activation, decrease of Bcl-2 expression, and elevation of Bax and caspase-3 expressions, suggesting that koumine might act directly on RAW 264.7 cells to inhibit LPS-induced apoptosis. It seems as though the mechanism that koumine possesses is the anti-apoptotic effect mediated by suppressing production of ROS, activation of p53, and mitochondrial apoptotic pathways in RAW 264 cells. Koumine could potentially serve as a protective effect against LPS-induced apoptosis.

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Section: Discussionsupporting

confidence: 94%

A Potential Mechanism for the Anti-Apoptotic Property of Koumine Involving Mitochondrial Pathway in LPS-Mediated RAW 264.7 Macrophages

Yuan

Liang

et al. 2016

Molecules

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“…This result was in accordance with the previous work (Yuan et al, ). According to previous findings, LPS induced apoptosis in macrophages through caspase‐dependent mitochondrial death pathway that decreased mitochondrial membrane potential and promoted activations of caspase‐3 and caspase‐9 (Langford, McGee, Ta, Redens, & Texada, ; Yuan et al, ). In this study, PSPPs treatment could notably reduce LPS‐induced apoptosis in macrophages.…”

Section: Discussionmentioning

confidence: 87%

Immunomodulatory effects of polysaccharides from purple sweet potato on lipopolysaccharide treated RAW 264.7 macrophages

et al. 2018

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Immunomodulatory effects of water-soluble polysaccharide (WSP), dilute alkali-soluble polysaccharide (DASP), and concentrated alkali-soluble polysaccharide (CASP) from purple sweet potato were evaluated by using lipopolysaccharide (LPS) treated RAW264.7 macrophage cell models. Results showed WSP, DASP, and CASP (100, 200, and 400 lg/mL) could suppress abnormal phagocytosis, overproduction of TNF-a and IL-6, and abnormal apoptosis caused by LPS. The high doses (200 and 400 lg/mL) of DASP and CASP could distinctly reduce excessive NO production as compared with LPS treatment group. In addition, 400 lg/mL of WSP, DASP, and CASP treatment groups exhibited dramatic decrease in the overexpression of IL-1b stimulated by LPS. Moreover, 200 and 400 lg/mL of WSP, DASP, and CASP markedly improved anti-inflammatory cytokine IL-10 as compared with LPS treatment group. Furthermore, 400 lg/mL of WSP and DASP treatments significantly increased the level of IL-10 in nonstimulated macrophages when compared with normal control group. Practical applicationsPurple sweet potato [Ipomoea batatas (L.) Lam] is one type of highly nutritional vegetable containing many vitamins, minerals, dietary fiber, anthocyanin, and polysaccharides. In addition, it is also one of health-promoting edible plant sources. Recently, polysaccharides have attracted extensive attentions due to their various biological activities. Our study reveals that polysaccharides extracted from purple sweet potato are effective in protecting macrophages damaged by lipopolysaccharide. Therefore, polysaccharides from purple sweet potato have anti-inflammatory efficacy.Thus, this study indicates the potential applications of purple sweet potato polysaccharides in functional food.

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“…Furthermore, in recent experimental animal models the exposure to LPS was associated to a significant increase in vacuolar degeneration, renal cell apoptosis and neutrophils and macrophages infiltration [22][23][24][25]. In particular, Langford et al observed in vitro multiple caspase activation in rabbit kidney cells treated with LPS, suggesting the stimulation of the extrinsic, intrinsic and endoplasmatic reticulum/stress apoptotic pathways [26]. Moreover, caspase inhibition has been shown to prevent apoptotic proximal, distal, and peri-tubular cell death in LPS animal models of septic acute kidney injury [11].…”

Section: Evaluation Of Plasma Immunemediated Moleculesmentioning

confidence: 99%

Cardiorenal Syndrome Type 5 in Sepsis: Role of Endotoxin in Cell Death Pathways and Inflammation

Virzì

Clementi

Brocca

et al. 2016

Kidney Blood Press Res

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Background/Aims: Cardiorenal Syndrome Type 5 (CRS Type 5) is characterized by concomitant cardiac and renal dysfunction in the setting of different systemic disorders, such as sepsis. In this study, we investigated the possible relationship between endotoxin levels, renal cell death and inflammation in septic patients with CRS Type 5. Methods: We enrolled 11 patients with CRS Type 5. CRS Type 5 was defined according to the current classification system. AKI was defined by Acute Kidney Injury Network (AKIN) criteria. Acute cardiac dysfunction was documented by echocardiography as acute left and/or right ventricular dysfunction leading to decreased ejection fraction. Endotoxin activity was measured by the Endotoxin Activity Assay (EAA). Plasma from CRS Type 5 patients was incubated with renal tubular cells (RTCs) and cell death levels were evaluated. Plasma cytokines levels were measured as well. Results: Accordingly to EAA levels, patients were divided into two groups: 45.4% of patients had low endotoxin activity level (negative EAA), while 54.5% of patients showed high endotoxin activity (positive EAA). RTCs incubated with plasma from EAA positive patients showed significantly higher apoptosis levels and higher caspase-3 activation compared to cells incubated with plasma from EAA negative patients, and a significant positive correlation was observed between EAA levels and RTC apoptosis levels. Furthermore, IL-6 and IFN-γ levels were significantly higher in CRS Type 5 patients with positive EAA. Conclusion: Our data suggest a possible relationship between endotoxin levels and renal cell death in septic patients with CRS Type 5. Furthermore, this study highlights the presence of renal apoptosis, the immune deregulation and the strong inflammation in CRS Type 5 patients, especially in those with high endotoxin activity.

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Multiple Caspases Mediate Acute Renal Cell Apoptosis Induced by Bacterial Cell Wall Components

Cited by 12 publications

References 43 publications

A Potential Mechanism for the Anti-Apoptotic Property of Koumine Involving Mitochondrial Pathway in LPS-Mediated RAW 264.7 Macrophages

A Potential Mechanism for the Anti-Apoptotic Property of Koumine Involving Mitochondrial Pathway in LPS-Mediated RAW 264.7 Macrophages

Immunomodulatory effects of polysaccharides from purple sweet potato on lipopolysaccharide treated RAW 264.7 macrophages

Cardiorenal Syndrome Type 5 in Sepsis: Role of Endotoxin in Cell Death Pathways and Inflammation

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