Multiple Crosslinking Hyaluronic Acid Hydrogels with Improved Strength and 3D Printability

Abstract

Hyaluronic acid (HA) hydrogel is preferred for biomedicine applications, as it possesses biodegradability, biocompatibility, and cell-regulated capacity as well as high hydration nature similar to the native extracellular matrix. However, HA hydrogel fabricated via a 3D printing technique often faces poor printing properties. In this study, maleiated sodium hyaluronate (MHA) with a high substituted degree of the acrylate group (i.e., 2.27) and thiolated sodium hyaluronate (SHHA) were synthesized. By blending these modified HAs, the MHA/SHHA hydrogels were prepared via pre-crosslinking through thiol–acrylate Michael addition and subsequently covalent crosslinking using thiol–acrylate and acrylate–acrylate photopolymerization mechanisms. Rheological properties, swelling behaviors, and mechanical properties can be modulated by altering the molar ratio of the thiol group and acrylate group. The results showed that the MHA/SHHA hydrogel precursors have rapidly gelling capacity and improved compressive strength. Based on these results, high-resolution hydrogel scaffolds with good structural stability were prepared by extrusion-based 3D printing. This HA hydrogel is cytocompatible and capable of supporting adherence of L929 cells, indicating its great potential for tissue engineering scaffolds.

Self-Healing Hyaluronic Acid Nanocomposite Hydrogels with Platelet-Rich Plasma Impregnated for Skin Regeneration

“…The PRP was donated by healthy human blood donors from the First Affiliated Hospital of Zhengzhou University. All the ELISA kits were purchased from 4A Biotech Co., Ltd. N -Hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC·HCl) were provided as described in our previous paper . All other reagents were A.R.…”

“…More importantly, due to the bioactivity of EGF in the stimulation of fibroblasts and keratinocytes to proliferate 63 and VEGF in the promotion of angiogenesis, 64 their sustained release in PRP T h i s c o n t e n t i s o n l y l i c e n s e d f o r c o n s u m and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC•HCl) were provided as described in our previous paper. 65 All other reagents were A.R. grade.…”

‘Two-faces’ of hyaluronan, a dynamic barometer of disease progression in tumor microenvironment

“…HA hydrogels containing a mixture of hyaluronan, collagen and matrigel could provide a similar ECM stiffness as mimicking the in vivo elasticity modulus of brain condition, which is further applying a better model for evaluating the malignancy of glioma [ 168 ]. With additional linkage reagents and other components of ECM, the model could repeat the ideal matrix for cultivating cells [ 175 ]. In chitosan-hyaluronan membrane-derived 3D culture models, the stemness phenotype is largely amplified as rapidly growing to tumor spheroids, increasing invasion and interstitialization.…”

Hyaluronic Acid as Bioink and Hydrogel Scaffolds for Tissue Engineering Applications

“…Various studies on HA modifications, such as methacrylated hyaluronic acid (MHA), dopamine-modified HA (HA-DA), HA-tyramine, thiolated hyaluronic acid (SHHA), catechol-HA, etc., have been reported for bioprinting of 3D tissue constructs . Wan et al used multiple cross-linking approaches to produce HA-based bioinks to print cellular constructs without incorporating other polymeric compositions . Two different HA modifications, i.e., MHA (formed by a ring-opening reaction between the OH group of HA and methacrylic anhydride) and SHHA, showed rapid pre-gelation and post-gelation kinetics.…”