Multiple Cycles of Preconditioning Cause Loss of Protection in Open-chest Rabbits

Iliodromitis, Efstathios K.; Kremastinos, Dimitrios Th.; Katritsis, Demosthenes G.; Papadopoulos, Constantinos; Hearse, David J.

doi:10.1006/jmcc.1996.0328

Cited by 66 publications

(50 citation statements)

References 3 publications

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“…We have therefore tried to increase the protection of IP by increasing the number of preconditioning ischemia-reperfusion cycles from one to four with the aim to possibly attenuate this greater protection with TNF-␣ antibodies. However, in agreement with other prior studies (1,8), a similar infarct size was obtained in three additional rabbits, which underwent four cycles of 5-min preconditioning ischemia with subsequent 5-min reperfusion before 30-min coronary artery occlusion and 180-min reperfusion (14 Ϯ 5%) as in rabbits with only a single cycle of preconditioning ischemia-reperfusion (13 Ϯ 7%).…”

Section: Discussionsupporting

confidence: 91%

TNF-α antibodies are as effective as ischemic preconditioning in reducing infarct size in rabbits

Belosjorow

Bolle

Duschin

et al. 2003

American Journal of Physiology-Heart and Circulatory Physiology

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Pretreatment with tumor necrosis factor-α (TNF-α) antibodies abolishes myocardial infarct size reduction by late ischemic preconditioning (IP). Whether or not TNF-α is also important for myocardial infarct size reduction by classic IP is unknown. Anesthetized rabbits were untreated ( group 1, n = 7), classically preconditioned by 5 min left coronary artery occlusion/10 min reperfusion ( group 2, n = 6), or pretreated with TNF-α antibodies without ( group 3, n = 6) or with IP ( group 4, n = 6) before undergoing 30 min of occlusion and 180 min of reperfusion. Infarct size in group 1 was 44 ± 11 (means ± SD)% of the area at risk. With a comparable area at risk, infarct size was reduced to 13 ± 7%, 23 ± 8%, and 19 ± 12% (all P < 0.05) in groups 2, 3, and 4, respectively. The circulating TNF-α concentration was increased during ischemia in group 1 from 752 ± 403 to 1,542 ± 482 U/ml ( P < 0.05) but remained unchanged in all other groups. Circulating TNF-α concentration during ischemia and infarct size correlated in all groups ( r = 0.76). IP, TNF-α antibodies, and the combined approach reduced infarct size to a comparable extent. Therefore, the question of whether or not TNF-α is causally involved in the infarct size reduction by IP in rabbits could not be answered.

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Section: Discussionsupporting

confidence: 91%

TNF-α antibodies are as effective as ischemic preconditioning in reducing infarct size in rabbits

Belosjorow

Bolle

Duschin

et al. 2003

American Journal of Physiology-Heart and Circulatory Physiology

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“…In addition, there were no statistically significant differences among any of are contradictory, it is impossible to establish a relation between repeated episodes of IPC and a reduction in I/R-related tissue damage. (21,22) Du et al, using in a rat model of unilateral transplantation, were the first to report decreased ROS production in lungs submitted to IPC. (23) The effect that IPC has on I/R-related lung injury was also examined by other authors in various animal models: of isolated lung reperfusion (21,24,25) ; of in situ I/R (26)(27)(28) ; and of lung transplantation.…”

Section: Resultsmentioning

confidence: 99%

Pré-condicionamento isquêmico por oclusão seletiva da artéria pulmonar em ratos

et al. 2008

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Objective: To evaluate the effect of lung ischemic preconditioning (IPC) on normothermic ischemia/reperfusion (I/R) injury in a rat model, quantifying the production of reactive oxygen species. Methods: Forty-seven male Wistar rats were randomized into four groups: control, sham, I/R and IPC. Control group animals were anesthetized and killed by decapitation, after which pneumonectomy was performed and the left lungs were stored in liquid nitrogen. Sham, IPC and I/R group rats were anesthetized, tracheostomized, ventilated, anticoagulated and submitted to left thoracotomy with dissection of the left pulmonary artery for clamping. Sham group rats underwent dissection of the left pulmonary artery, I/R group rats underwent 30 min of total hilar clamping, and IPC group rats underwent 5-min clamping of the left pulmonary artery followed by 30 min of total hilar clamping. Lungs were reperfused for 90 min and ventilated with the same parameters, with additional positive end-expiratory pressure of 1 cmH 2 O. Hemodynamic and blood gas values were obtained prior to thoracotomy, prior to total hilar clamping, after 30 min of reperfusion and after 90 min of reperfusion. Lipid peroxidation was determined by measuring levels of thiobarbituric acid reactive substances. Results: There were no significant differences among the groups in terms of the levels of thiobarbituric acid reactive substances. Nor were there any significant differences among the sham, I/R and IPC groups in terms of arterial oxygen tension, arterial carbon dioxide tension or hemodynamic values. Conclusions: In an in situ I/R rat model, 5-min IPC of the left pulmonary artery does not attenuate I/R injury.Keywords: Ischemia; Reperfusion; Organ preservation; Reactive oxygen species. ResumoObjetivo: Avaliar o efeito do pré-condicionamento isquêmico (PCI) em modelo de isquemia e reperfusão (I/R) pulmonar normotérmica em ratos, quantificando a produção de espécies reativas do oxigênio. Métodos: Quarenta e sete ratos Wistar foram randomizados em quatro grupos: controle, sham, I/R e PCI. Após anestesia, animais do grupo controle foram sacrificados por decapitação, pneumonectomizados, e os pulmões esquerdos armazenados em nitrogênio líquido. Animais dos grupos sham, I/R e PCI foram anestesiados, traqueostomizados, ventilados, anticoagulados e submetidos a uma toracotomia esquerda com dissecção da artéria pulmonar esquerda para clampeamento. No grupo sham procedeu-se a dissecção da artéria pulmonar esquerda; no grupo I/R, clampeamento hilar total de 30 min e no grupo PCI, clampeamento da artéria pulmonar esquerda por 5 min seguido por reperfusão de 10 min e um clampeamento hilar total de 30 min. Pulmões foram reperfundidos por 90 min e ventilados com os mesmos parâmetros, acrescidos de pressão expiratória final positiva de 1 cmH 2 O. Foram obtidas medidas hemodinâmicas e gasométricas antes da toracotomia, antes do clampeamento hilar total, aos 30 e 90 min de reperfusão. A peroxidação lipídica foi estabelecida por meio da determinação das substâncias reativas ...

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“…Reperfusion was achieved by unclamping the tube. 24) Experimental Protocol: Rabbits were randomly divided in one control group (nϭ5) that received normal saline and to groups treated with the synthesized compounds. After the period of hemodynamic stabilization rabbit hearts were assigned to 30 min of sustained ischemia followed by reperfusion.…”

Section: Methodsmentioning

confidence: 99%

Antioxidant Activity of Novel Indole Derivatives and Protection of the Myocardial Damage in Rabbits.

Andreadou

Tasouli

Bofilis

et al. 2002

CHEMICAL & PHARMACEUTICAL BULLETIN

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Novel indole derivatives containing a triazole moiety (1a-d, 2a-c) were synthesized as lead compounds with interesting pharmacological profiles. Their antioxidant activity was investigated on in vitro non-enzymatic rat hepatic microsomal lipid peroxidation. All compounds showed significant effect in the above assay. The effect depended mainly on the attachment position of the triazole moiety on the indole nucleus. The most potent antioxidant derivatives 1a, 1c and 1d were tested for their protective ability against the oxidative damage of the myocardium after ischemia-reperfusion, in male rabbits which were subjected to 30 min regional ischemia followed by reperfusion. The tested antioxidant compounds 1a, 1c and 1d were continuously infused for 30 min starting at 10th min of ischemia and lasted at 10th min of reperfusion. The concentration of malondialdehyde (MDA, a marker of lipid peroxidation) and hemodynamic parameters (blood pressure and heart rate) were measured in the baseline, at 20th min of the sustained ischemia, 1st and 20th min of reperfusion. It was found that the examined compounds 1a, 1c and 1d reduced significantly the level of MDA in rabbits under ischemia-reperfusion and proved to be promising substances for further evaluation of anti-ischemic properties.

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Multiple Cycles of Preconditioning Cause Loss of Protection in Open-chest Rabbits

Cited by 66 publications

References 3 publications

TNF-α antibodies are as effective as ischemic preconditioning in reducing infarct size in rabbits

TNF-α antibodies are as effective as ischemic preconditioning in reducing infarct size in rabbits

Pré-condicionamento isquêmico por oclusão seletiva da artéria pulmonar em ratos

Antioxidant Activity of Novel Indole Derivatives and Protection of the Myocardial Damage in Rabbits.

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