Multiple cyclin-dependent kinase complexes and phosphatases control G2/M progression in alfalfa cells

Mészáros, Tamás; Miskolczi, Pál; Ayaydin, Ferhan; Pettkó‐Szandtner, Aladár; Peres, Adrian; Magyar, Zoltán; Horváth, Gábor; Bako, L.; Fehér, Attila; Dudits, Dénes

doi:10.1007/978-94-010-0936-2_5

Cited by 30 publications

(61 citation statements)

References 47 publications

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“…Moreover, the tomato CDKB2 could bind CycA2. In our hands, CDKB1 failed to interact with any of the tested cyclins, unlike the alfalfa CDKB1, which binds a cyclin D3 (Mészáros et al, 2000). We were unable to reveal any interaction between Lyces; CDKC;1 and mitotic and G1 cyclins.…”

Section: Discussioncontrasting

confidence: 73%

“…A very faint signal could still be observed up to the MG stage for Lyces;CDKB1;1, whereas Lyces; CDKB2;1 transcripts became undetectable after 15 DPA. This slight difference in the gene expression may originate from the S/G2 and G2/M phase dependence at the transcriptional level of CDKB1 and CDKB2, respectively (Mironov et al, 1999;Mészáros et al, 2000). In Arabidopsis, the only CDKB gene so far studied, CDC2b, corresponds to a member of the CDKB1 family (Arath;CDKB1;1, Joubés et al, 2000a).…”

Section: Discussionmentioning

confidence: 99%

“…Using the yeast two-hybrid system, different authors demonstrated the protein-protein interactions between CDKA or CDKB and various D-type cyclins (De Veylder et al, 1997Nakagami et al, 1999;Meijer and Murray, 2000;Mészáros et al, 2000), CDKA and CycA2 (Roudier et al, 2000), and CDKD and its specific partners CycH and CycC (Yamaguchi et al, 2000). However, no exhaustive analysis of plant CDK/cyclin binding has been performed so far.…”

Section: Discussionmentioning

confidence: 99%

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A New C-Type Cyclin-Dependent Kinase from Tomato Expressed in Dividing Tissues Does Not Interact with Mitotic and G1 Cyclins

et al. 2001

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Cyclin-dependent kinases (CDKs) form a conserved superfamily of eukaryotic serine-threonine protein kinases whose activity requires the binding of a cyclin protein. CDKs are involved in many aspects of cell biology and notably in the regulation of the cell cycle. Three cDNAs encoding a C-type CDK, and a member of each B-type CDK subfamily, were isolated from tomato (Lycopsersicon esculentum Mill.) and designated Lyces;CDKC;1 (accession no. AJ294903), Lyces; CDKB1;1 (accession no. AJ297916), and Lyces;CDKB2;1 (accession no. AJ297917). The predicted amino acid sequences displayed the characteristic PITAIRE (CDKC), PPTALRE (CDKB1), and PPTTLRE (CDKB2) motives in the cyclin-binding domain, clearly identifying the type of CDK. The accumulation of all transcripts was associated preferentially with dividing tissues in developing tomato fruit and vegetative organs. In contrast to that of CDKA and CDKBs, the transcription pattern of Lyces;CDKC;1 was shown to be independent of hormone and sugar supply in tomato cell suspension cultures and excised roots. This observation, together with the absence of a patchy expression profile in in situ hybridization experiments, suggests a non-cell cycle regulation of Lyces;CDKC;1. Using a two-hybrid assay, we showed that Lyces;CDKC;1 did not interact with mitotic and G1 cyclins. The role of plant CDKCs in the regulation of cell division and differentiation is discussed with regard to the known function of their animal counterparts.

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Section: Discussioncontrasting

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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A New C-Type Cyclin-Dependent Kinase from Tomato Expressed in Dividing Tissues Does Not Interact with Mitotic and G1 Cyclins

et al. 2001

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“…In addition, recently, CDKA;1 was shown to be directly phosphorylated by WEE1 in vitro in a Tyr-15-dependent manner (Shimotohno et al, 2006). Similarly, in a cell suspension culture of alfalfa (Medicago sativa), PSTAIREholding CDKs have been found to undergo Tyr phosphorylation (Mé szá ros et al, 2000). In mammalian cells, the drug caffeine cancels the DNA replication checkpoint through inhibition of the ATM kinase (Schlegel and Pardee, 1986;Andreassen and Margolis, 1992;Blasina et al, 1999), but in plants, it overrides the replication checkpoint only in the presence of the mitotic cyclin CYCB2 (Weingartner et al, 2003).…”

Section: Discussionmentioning

confidence: 98%

ArabidopsisWEE1 Kinase Controls Cell Cycle Arrest in Response to Activation of the DNA Integrity Checkpoint

Schutter¹,

Joubès²,

et al. 2007

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Upon the incidence of DNA stress, the ataxia telangiectasia-mutated (ATM) and Rad3-related (ATR) signaling kinases activate a transient cell cycle arrest that allows cells to repair DNA before proceeding into mitosis. Although the ATM-ATR pathway is highly conserved over species, the mechanisms by which plant cells stop their cell cycle in response to the loss of genome integrity are unclear. We demonstrate that the cell cycle regulatory WEE1 kinase gene of Arabidopsis thaliana is transcriptionally activated upon the cessation of DNA replication or DNA damage in an ATR-or ATM-dependent manner, respectively. In accordance with a role for WEE1 in DNA stress signaling, WEE1-deficient plants showed no obvious cell division or endoreduplication phenotype when grown under nonstress conditions but were hypersensitive to agents that impair DNA replication. Induced WEE1 expression inhibited plant growth by arresting dividing cells in the G2-phase of the cell cycle. We conclude that the plant WEE1 gene is not rate-limiting for cycle progression under normal growth conditions but is a critical target of the ATR-ATM signaling cascades that inhibit the cell cycle upon activation of the DNA integrity checkpoints, coupling mitosis to DNA repair in cells that suffer DNA damage.

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“…Recent molecular biological and biochemical evidence indicates that coordinated interactions between coupled protein kinases and phosphatases also play important roles in plants, especially in cell cycle control, in which a set of cyclin-dependent kinases and protein phosphatases are involved (Meszaros et al, 2000;Boniotti and Griffith, 2002). It also has been suggested that protein phosphorylation is an essential step in light signal transduction in plants and that protein phosphatase(s) possibly is involved in this process (Sheen, 1993;Harter et al, 1994;Chandok and Sopory, 1996;Fankhauser, 2000).…”

Section: Phytochrome Kinases Interact Functionally With a Pp2amentioning

confidence: 99%

A Phytochrome-Associated Protein Phosphatase 2A Modulates Light Signals in Flowering Time Control in Arabidopsis

et al. 2002

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Multiple cyclin-dependent kinase complexes and phosphatases control G2/M progression in alfalfa cells

Cited by 30 publications

References 47 publications

A New C-Type Cyclin-Dependent Kinase from Tomato Expressed in Dividing Tissues Does Not Interact with Mitotic and G1 Cyclins

A New C-Type Cyclin-Dependent Kinase from Tomato Expressed in Dividing Tissues Does Not Interact with Mitotic and G1 Cyclins

ArabidopsisWEE1 Kinase Controls Cell Cycle Arrest in Response to Activation of the DNA Integrity Checkpoint

A Phytochrome-Associated Protein Phosphatase 2A Modulates Light Signals in Flowering Time Control in Arabidopsis

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