Multiple dyes applications for fluorescent convertible polymer capsules as macrophages tracking labels

Kozyreva, Zhanna V.; Demina, Polina A.; Sapach, Anastasiia Yu; Terentyeva, Daria A.; Gusliakova, Olga I.; Abramova, Anna M.; Goryacheva, Irina Yu; Trushina, Daria B.; Sukhorukov, Gleb B.; Sindeeva, Olga A.

doi:10.1016/j.heliyon.2024.e30680

Cited by 4 publications

(4 citation statements)

References 55 publications

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“…Moreover, λ-scans of capsules (Figure 2C) demonstrated a blue shift of capsules' spectra. It gave the opportunity to detect capsules in different ranges and was also reported previously [19,22]. Therefore, the optimal parameters of photoconversion of capsules containing RhB using a 561 nm laser are LPD 451 kW/cm 2 and a one pixel irradiation duration of 0.4 ms.…”

Section: Selection Of Optimal Photoconversion Parameters Using Confoc...mentioning

confidence: 54%

“…Synthesis of photoconvertible polymer microcapsules was done according to a previously described procedure [22]. Initially, vaterite cores were prepared by mixing calcium chloride dihydrate and sodium carbonate salts (1 M, 0.650 μL) in 3 mL of water.…”

Section: Synthesis and Characterization Of Photoconvertible Microcaps...mentioning

confidence: 99%

“…Polymer microcapsules containing RhB were synthesized according to the procedure described earlier [22,23]. On their base, three types of capsules were prepared with some modifications (Figure 1A).…”

Section: Synthesis and Characterization Of Polyelectrolyte Fluorescen...mentioning

confidence: 99%

“…These capsules can be internalized by cells and irreversibly photoconverted inside of them using localized laser exposure. Subsequently, the synthesis technique was improved, significantly increasing the number of capsules in the sample (250 times) due to heat treatment in a polyvinyl alcohol gel environment [22]. In this work, a number of dyes capable of photoconversion after encapsulation during hydrothermal synthesis were also selected and described.…”

Section: Introductionmentioning

confidence: 99%

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Tracking Single Mouse Mesenchymal Stromal Cells Migration into Glioblastoma using a Photoconvertible Microcapsules

Sindeeva,

Demina,

Kozyreva

et al. 2024

Preprint

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To elucidate mesenchymal stromal cells (MSC)-tumor intricate and ambiguous interactions, the reliable cell labeling and tracking techniques are imperative. Traditional methods of cell labelling have limitations mostly relevant to need for genetic modification what demands the exploration for alternative strategies. Here, we propose using fluorescent photoconvertible microcapsules containing Rhodamine B (RhB) to study mMSC migration in brain tumors. These 3 μm sized microcapsules are readily internalised by cells and undergo photoconversion under 561 nm laser exposure with fluorescence blue shift on demand. Optimal photoconversion was achieved with a laser irradiation duration of 0.4 ms, which provide a maximal brightness of photoconverted label without an excessive laser exposure on cells. Capsules modified with polyallylamine hydrochloride demonstrated the best potential for intracellular uptake without significantly affecting cell viability, motility, or proliferation. The optimal ratio of 20 capsules per mMSC was determined for labeling. Moreover, migration of individual mMSCs within 2D and 3D glioblastoma cell (EPNT-5) colonies over 2 days and in vivo tumor settings over 7 days was tracked. Our study unveils a robust platform for investigating MSC-tumor dynamics, offering insights into therapeutic strategies. Photoconvertible microcapsules could also become an indispensable tool for studying complex fundamental processes of cell-cell interactions for a wide range of problems in biology and medicine.

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Section: Selection Of Optimal Photoconversion Parameters Using Confoc...mentioning

confidence: 54%

Section: Synthesis and Characterization Of Photoconvertible Microcaps...mentioning

confidence: 99%

Section: Synthesis and Characterization Of Polyelectrolyte Fluorescen...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Tracking Single Mouse Mesenchymal Stromal Cells Migration into Glioblastoma using a Photoconvertible Microcapsules

Sindeeva,

Demina,

Kozyreva

et al. 2024

Preprint

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Exchange of free and capsule conjugated cyanine dyes between cells

Kozyreva,

Demina,

Gusliakova

et al. 2024

J. Mater. Chem. B

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Long-term tracing of individual human neural cells using multiphoton microscopy and photoconvertible polymer capsules

Smirnov,

Usatova,

Berestovoy

et al. 2024

J. R. Soc. Interface.

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The study of human neural cells, their behaviour and migration are important areas of research in the biomedical field, particularly for potential therapeutic applications. The safety of using neural cells in therapy is still a concern due to a lack of information on long-term changes that may occur. While current methods of cell tracing explore gene manipulations, we elaborate approaches to cell marking with no genetic interference. In this study, we present a novel method for labelling and tracking neural cells using cell-impregnatable photoconvertible polyelectrolyte microcapsules. These capsules demonstrated low cytotoxicity with no effect on the differentiation ability of the neural cells, maintained a high level of fluorescent signal and ability for tracing individual neural cells for over 7 days. The capsules modified with rhodamine- and fluorescein-based dyes were demonstrated to undergo photoconversion by both one- and two-photon lasers while being internalized by neural cells. The finding gives the possibility to select individual capsules inside multicellular structures like spheroids and tissues and alternate their fluorescent appearance. Thus, we can track individual cell paths in complex systems. This new method offers a promising alternative for studying neural cells’ long-term behaviour and migration in complex systems such as three-dimensional cellular populations.

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Multiple dyes applications for fluorescent convertible polymer capsules as macrophages tracking labels

Cited by 4 publications

References 55 publications

Tracking Single Mouse Mesenchymal Stromal Cells Migration into Glioblastoma using a Photoconvertible Microcapsules

Tracking Single Mouse Mesenchymal Stromal Cells Migration into Glioblastoma using a Photoconvertible Microcapsules

Exchange of free and capsule conjugated cyanine dyes between cells

Long-term tracing of individual human neural cells using multiphoton microscopy and photoconvertible polymer capsules

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