Multiple Fingerprint Profiles and Chemometrics Analysis of Polysaccharides From the Roots of <i>Glehnia littoralis</i>

Jing, Yongshuai; Zhang, Hao; Zhang, Ruijuan; Su, Lei; Hu, Beibei; Zhang, Danshen; Zheng, Yuguang; Wu, Liqing

doi:10.1177/1934578x221106902

Cited by 4 publications

(2 citation statements)

References 24 publications

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“…The samples were separately dissolved in 0.5 mL of DMSO, and then 1 H NMR was recorded on an AVANCE NEO 500 MHz spectrometer (Bruker, Mannheim, Germany) with a 5 mm CPTCI 1 H probe [22].…”

Section: Nmr Analysis Of Pspsmentioning

confidence: 99%

Effects of Extraction Methods on the Physicochemical Properties and Biological Activities of Polysaccharides from Polygonatum sibiricum

Jing,

Yan,

Zhang

et al. 2023

Foods

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Polygonatum sibiricum polysaccharides (PSPs) have important biological functions, such as antioxidation, immunomodulatory, and hypolipidemic functions. Different extraction methods have effects on their structures and activities. In this study, six extraction methods, including hot water extraction (HWE), alkali extraction (AAE), ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), microwave-assisted extraction (MAE), and freeze–thaw-assisted extraction (FAE) were used to extract PSPs, and their structure–activity relationships were analyzed. The results showed that all six PSPs had similar functional group compositions, thermal stability, and glycosidic bond compositions. PSP-As (PSPs extracted by AAE) exhibited better rheological properties due to their higher molecular weight (Mw). PSP-Es (PSPs extracted by EAE) and PSP-Fs (PSPs extracted by FAE) had better lipid-lowering activity due to their lower Mw. PSP-Es and PSP-Ms (PSPs extracted by MAE), which do not contain uronic acid and have a moderate Mw, had better 1,1-diphenyl-2-picrylhydrazyl (DPPH)-radical-scavenging activity. On the contrary, PSP-Hs (PSPs extracted by HWE) and PSP-Fs, with the Mw of uronic acid, had the best OH-radical-scavenging activity. The high-Mw PSP-As had the best Fe2+-chelating ability. In addition, mannose (Man) may play an important role in the immunomodulatory activity. These results indicate that different extraction methods affect the structure and biological activity of polysaccharides to varying degrees, and these results are helpful for understanding the structure–activity relationship of PSPs.

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“…The samples were separately dissolved in 0.5 mL of DMSO, and then 1 H NMR was recorded on an AVANCE NEO 500 MHz spectrometer (Bruker, Mannheim, Germany) with a 5 mm CPTCI 1 H probe [22].…”

Section: Nmr Analysis Of Pspsmentioning

confidence: 99%

Effects of Extraction Methods on the Physicochemical Properties and Biological Activities of Polysaccharides from Polygonatum sibiricum

Jing,

Yan,

Zhang

et al. 2023

Foods

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“…Therefore, the present study provide a sensitive and accurate DNA molecular method for quality control of G. littoralis and its industrial products, in comparison with the other molecular protocols so far used. 4…”

Section: Discussionmentioning

confidence: 99%

Development of a reliable DNA method for quality control of Glehnia littoralis and its industrial extracts

Lin

Wang

et al. 2022

Preprint

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As a medicinal and edible plant, Glehnia littoralis has been widely recognized for its comprehensive nutritional properties and pharmaceutical effects, but it has also been the target of fraudulent practices. To overcome the difficulties in unanimous polymorphism exploitation of the adulterants and DNA degradation of industrial extracts, ribosomal ITS and chloroplast trnL-F gene sequences were targeted for molecular analysis. Based on the developed single nucleotide polymorphism (SNP) markers, two species-specific PCR systems were respectively established and proved to be effective for molecular discrimination of G. littoralis from its adulterants. The SNP marker exploited in trnL-F region was able to detect 0.1% of intentional adulteration of genomic DNA with a detection limit of 0.01 ng, and it is effective for botanical origin authentication of G. littoralis industrial extracts either in single or mixed plant samples. Compared with the other molecular protocols so far used, the established DNA method enabled the accurate authentication and standardization of routine work for quality control of G. littoralis and its industrial products.

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A sensitive and robust DNA method for authenticity determination of Glehnia littoralis F. Schmidt ex Miq. and its commercial products

Lin

Wang

et al. 2023

Journal of Applied Research on Medicinal and Aromatic Plants

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Multiple Fingerprint Profiles and Chemometrics Analysis of Polysaccharides From the Roots of Glehnia littoralis

Cited by 4 publications

References 24 publications

Effects of Extraction Methods on the Physicochemical Properties and Biological Activities of Polysaccharides from Polygonatum sibiricum

Effects of Extraction Methods on the Physicochemical Properties and Biological Activities of Polysaccharides from Polygonatum sibiricum

Development of a reliable DNA method for quality control of Glehnia littoralis and its industrial extracts

A sensitive and robust DNA method for authenticity determination of Glehnia littoralis F. Schmidt ex Miq. and its commercial products

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