Multiple Forms and Functions of Premature Termination by RNA Polymerase II

Bentley, David L.

doi:10.1016/j.jmb.2024.168743

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2025

Publication Types

Select...

Article2

Preprint1

Relationship

Self Cite0

Independent3

Authors

Journals

Cited by 3 publications

References 144 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

The Integrator complex: an emerging complex structure involved in the regulation of gene expression by targeting RNA polymerase II

Li,

Zeng,

et al. 2024

Funct Integr Genomics

View full text Add to dashboard Cite

The Integrator complex: an emerging complex structure involved in the regulation of gene expression by targeting RNA polymerase II

Li,

Zeng,

et al. 2024

Funct Integr Genomics

View full text Add to dashboard Cite

Controlling Transcription Elongation and Termination: More Than a Means to An End

Buratowski,

Arndt

2025

Journal of Molecular Biology

View full text Add to dashboard Cite

Mammalian promoters are characterised by low occupancy and high turnover of RNA polymerase II

Chatsirisupachai,

Moene,

Kleinendorst

et al. 2024

Preprint

View full text Add to dashboard Cite

The general transcription machinery and its occupancy at promoters are highly conserved across metazoans. This contrasts with the kinetics of mRNA production that considerably differ between model species such asDrosophilaand mouse. The molecular basis for these kinetic differences is currently unknown. Here, we used Single Molecule Footprinting to measure RNA Polymerase II (Pol II) occupancy, the fraction of DNA molecules bound, at promoters in mouse andDrosophilacell lines. Single molecule data reveals that Pol II occupancy is on average 3-5 times more frequent at transcriptionally activeDrosophilapromoters than active mouse promoters. Kinetic modelling of the occupancy states suggests that these differences in Pol II occupancy are determined by the ratio between the transcription initiation and Pol II turnover rates. We used chemical perturbation of transcription initiation to determine Pol II turnover rate in both species. Integration of these data into the model shows that infrequent Pol II occupancy in mammals is explained by the combination of high Pol II turnover and low transcription initiation rates.

show abstract

Multiple Forms and Functions of Premature Termination by RNA Polymerase II

Cited by 3 publications

References 144 publications

The Integrator complex: an emerging complex structure involved in the regulation of gene expression by targeting RNA polymerase II

The Integrator complex: an emerging complex structure involved in the regulation of gene expression by targeting RNA polymerase II

Controlling Transcription Elongation and Termination: More Than a Means to An End

Mammalian promoters are characterised by low occupancy and high turnover of RNA polymerase II

Contact Info

Product

Resources

About