Extraction and display of frequency information in three-dimensional (3D) acoustic data are important steps to analyze object characteristics, because the characteristics, such as profiles, sizes, surface structures, and material properties, may show frequency dependence. In this study, frequency representation (FR) based on phase information in multispectral acoustic imaging (MSAI) is proposed to overcome the limit of intensity or amplitude information in image display. Experiments are performed on 3D acoustic data collected from a rigid surface engraved with five different letters. The results show that the proposed FR technique can not only identify the depth of the five letters by the colors representing frequency characteristics but also demonstrate the 3D image of the five letters, providing more detailed characteristics that are unavailable by conventional acoustic imaging.