Multiple functions of a Drosophila homeotic gene, zeste-white 3, during segmentation and neurogenesis

Perrimon, Norbert; Smouse, David

doi:10.1016/0012-1606(89)90180-2

Cited by 74 publications

(51 citation statements)

References 65 publications

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“…The sgg gene also has multiple functions in the embryo; in addition to regulating En maintenance, it is required for normal organization of the nervous system (Perrimon and Smouse 1989). Loss of eld leads to a similar disorganization that is considerably less extreme than the overproduction of neuroblasts seen in N mutants (data not shown).…”

Section: Interactions Between Signaling Pathwaysmentioning

confidence: 89%

“…Ectopic bristle formation was restricted to the dorsal surface of the wing, within the anterior compartment, and was observed most commonly near the wing margin in tufts of the bristle type appropriate to their position along the anterior-posterior axis. Differentiation of the wing margin is controlled by the wg pathway, and ectopic wing margin bristles are produced in clones mutant for sgg (Simpson et al 1988;Perrimon and Smouse 1989;. However, sgg clones show neither the non-autonomy nor the positional restrictions observed for eld clones.…”

Section: Eld Affects Wing Patterningmentioning

confidence: 99%

“…Interestingly, the provision of a paternal wild-type copy of the eld gene failed to rescue the maternal mutants and made no perceptible difference to the phenotype (data not shown), suggesting either that early expression of eld is critical for its function or that the level of expression of the paternal copy is insufficient. Similarly, embryos maternally mutant for sgg are only partially rescued by a wild-type paternal copy of the gene (Perrimon and Smouse 1989;Siegfried et al 1992), sug- gesting that antagonism of the wg pathway may require high levels of gene expression.…”

Section: Eld Is Required In Embryonic Segmentationmentioning

confidence: 99%

“…There also appears to be a negative interaction between wg and N signaling at the wing margin; Dsh, which interacts physically with the intracellular domain of N, inhibits N function, thereby limiting the domain of wg expression (Axelrod et al 1996;Rulifson et al 1996). In addition, the same gene, sgg/zw3, was isolated independently as an inhibitory element of the Wg pathway and a positive element of the N pathway (Bourouis et al 1989;Perrimon and Smouse 1989).…”

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confidence: 99%

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eyelidantagonizes wingless signaling during Drosophiladevelopment and has homology to the Bright family of DNA-binding proteins

Treisman

Luk²,

Rubin³

et al. 1997

Genes Dev.

115

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In Drosophila, pattern formation at multiple stages of embryonic and imaginal development depends on the same intercellular signaling pathways. We have identified a novel gene, eyelid (eld), which is required for embryonic segmentation, development of the notum and wing margin, and photoreceptor differentiation. In these tissues, eld mutations have effects opposite to those caused by wingless (wg) mutations. eld encodes a widely expressed nuclear protein with a region homologous to a novel family of DNA-binding domains. Based on this homology and on the phenotypic analysis, we suggest that Eld could act as a transcription factor antagonistic to the Wg pathway.

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Section: Interactions Between Signaling Pathwaysmentioning

confidence: 89%

Section: Eld Affects Wing Patterningmentioning

confidence: 99%

Section: Eld Is Required In Embryonic Segmentationmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

eyelidantagonizes wingless signaling during Drosophiladevelopment and has homology to the Bright family of DNA-binding proteins

Treisman

Luk²,

Rubin³

et al. 1997

Genes Dev.

115

View full text Add to dashboard Cite

show abstract

“…Xenopus GSK-3 functions as a member of the Wnt signaling pathway, determines cell fate, and regulates axis formation during early development (He et al, 1995;Yost et al, 1996). The Drosophila zeste-white3/shaggy gene product is homologous to GSK-3␤ (Ruel et al, 1993) and is required at several developmental stages during fly embryogenesis (Simpson et al, 1988;Perrimon and Smouse, 1989). A Dictyostelium homologue of GSK-3 has been found to be important for cellular differentiation (Harwood et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Yeast Glycogen Synthase Kinase-3 Activates Msn2p-dependent Transcription of Stress Responsive Genes

Hirata

Andoh²,

Asahara

et al. 2003

MBoC

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The yeast Saccharomyces cerevisiae has four genes, MCK1, MDS1 (RIM11), MRK1, and YOL128c, that encode homologues of mammalian glycogen synthase kinase 3 (GSK-3). A gsk-3 null mutant in which these four genes are disrupted showed growth defects on galactose medium. We isolated several multicopy suppressors of this growth defect. Two of them encoded Msn2p and phosphoglucomutase (PGM). Msn2p is a transcription factor that binds to the stress-response element (STRE). PGM is an enzyme that interconverts glucose-1 phosphate and glucose-6 phosphate and is regulated by Msn2p at the transcriptional level. Expression of the mRNAs of PGM2 and DDR2, whose promoter regions possess STRE sequences, on induction by heat shock or salt stress was reduced not only in an msn2 msn4 (msn2 homologue) double mutant but also in the gsk-3 null mutant. STRE-dependent transcription was greatly inhibited in the gsk-3 null mutant or mck1 mds1 double mutant, and this phenotype was suppressed by the expression of Mck1p but not of a kinase-inactive form of Mck1p. Although Msn2p accumulated in the nucleus of the gsk-3 null mutant as well as in the wild-type strain under various stress conditions, its STRE-binding activity was reduced in extracts prepared from the gsk-3 null mutant or mck1 mds1 double mutant. These results suggest that yeast GSK-3 promotes formation of a complex between Msn2p and DNA, which is required for the proper response to different forms of stress. Because neither Msn2p–GSK-3 complex formation nor GSK-3–dependent phosphorylation of Msn2p could be detected, the regulation of Msn2p by GSK-3 may be indirect

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An early embryonic product of the gene shaggy encodes a serine/threonine protein kinase related to the CDC28/cdc2+ subfamily.

et al. 1990

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The product(s) of the gene shaggy (sgg) is required for seemingly unrelated events during the development of Drosophila melanogaster. In embryos, maternal and zygotically derived sgg products are required initially to construct a normal syncytial blastoderm and later for normal segmentation. Furthermore, in mutant animals a process of intercellular communication that is required for the segregation of the neural and epidermal lineage during the formation of the central nervous system and the adult peripheral nervous system is disrupted. Here we describe a transcription unit of approximately 40 kb lying within the cloned chromosomal interval 3B1, and provide evidence that it encodes the sgg+ function. Of seven developmentally regulated transcripts that are partially generated by alternative splicing, two seem to be responsible for early sgg activity. Sequence analysis of corresponding cDNA(s) predicts a protein of 514 amino acids with a canonical catalytic domain found in serine/threonine specific protein kinases, linked to an unusual region rich in Gly, Ala and Ser. A search for homologies as well as a comparative study of the kinase catalytic domain with that of other proteins, revealed that the protein kinase domain of sgg is distantly related to the members of the CDC28/cdc2+ subfamily of protein kinases, all of which play cardinal roles in the regulation of the yeast and mammalian cell cycles. Ubiquitous expression of sgg transcripts was found during embryonic stages. A possible role of the sgg protein in a signal transduction pathway necessary for intercellular communication at different stages of development is discussed.

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Multiple functions of a Drosophila homeotic gene, zeste-white 3, during segmentation and neurogenesis

Cited by 74 publications

References 65 publications

eyelidantagonizes wingless signaling during Drosophiladevelopment and has homology to the Bright family of DNA-binding proteins

eyelidantagonizes wingless signaling during Drosophiladevelopment and has homology to the Bright family of DNA-binding proteins

Yeast Glycogen Synthase Kinase-3 Activates Msn2p-dependent Transcription of Stress Responsive Genes

An early embryonic product of the gene shaggy encodes a serine/threonine protein kinase related to the CDC28/cdc2+ subfamily.

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