Multiple Functions of Compatible Solute Ectoine and Strategies for Constructing Overproducers for Biobased Production

Zhang, Wei; Liu, Kun; Kong, Fang; Tao, Ye; Wang, Tianwen

doi:10.1007/s12033-023-00827-7

Mol Biotechnol

2023

DOI: 10.1007/s12033-023-00827-7

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Multiple Functions of Compatible Solute Ectoine and Strategies for Constructing Overproducers for Biobased Production

Wei Zhang¹,

Kun Liu²,

Fang Kong³

et al.

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2024

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Cited by 2 publications

References 128 publications

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Improving salt-tolerant artificial consortium of Bacillus amyloliquefaciens for bioconverting food waste to lipopeptides

Zhang,

Qiao,

Shang

et al. 2024

Waste Management

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Improving salt-tolerant artificial consortium of Bacillus amyloliquefaciens for bioconverting food waste to lipopeptides

Zhang,

Qiao,

Shang

et al. 2024

Waste Management

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Efficient Biosynthesis of Ectoine in Recombinant Escherichia coli by Biobrick Method

Naeem,

Yuan,

Luo

et al. 2024

Fermentation

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Ectoine is a compatible solute naturally produced in some halophilic bacteria as a protective agent for survival in salty environments. It has gained special interest as a therapeutic agent in the pharmaceutical and healthcare sectors for the treatment of different diseases. Ectoine mainly produced by bacterial milking, chemical, and fed-batch fermentation methods under a high-salt medium. Unfortunately, the ectoine yield through these methods is still too low to meet high industrial demand, causing salinity issues. The biobrick method was potentially utilized for efficient ectoine biosynthesis under a low-salt medium with different conditions in E. coli BL21(DE3) harboring the pET-22bNS-EctA-EctB-EctC plasmid. Firstly, three genes, L-2,4-diamino-butyric acid acetyltransferase (ectA), L-2,4-diaminobutyric acid transaminase (ectB), and ectoine synthase (ectC) from Bacillus pseudofirmus OF4, were precisely assembled and expressed into E. coli BL21(DE3). After optimizing the reaction conditions in a whole-cell catalytic reaction [50 mM of the sodium phosphate buffer (pH~7.5) containing 300 mM L-aspartic acid, 100 mM glycerol, 1/20 g/mL cell pellets], the amount of ectoine in the plasmid pET-22bNS-ALacBTacCTac reached the maximum level of 167.2 mg/mL/d (6.97 mg/mL/h). Moreover, Western blot analysis revealed that high expression levels of EctA and EctC had a significant effect on ectoine biosynthesis, indicating that both proteins might be the key enzymes in ectoine production. We conclude that a high amount of ectoine achieved through the biobrick method and efficiently used for different industrial applications.

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Multiple Functions of Compatible Solute Ectoine and Strategies for Constructing Overproducers for Biobased Production

Cited by 2 publications

References 128 publications

Improving salt-tolerant artificial consortium of Bacillus amyloliquefaciens for bioconverting food waste to lipopeptides

Improving salt-tolerant artificial consortium of Bacillus amyloliquefaciens for bioconverting food waste to lipopeptides

Efficient Biosynthesis of Ectoine in Recombinant Escherichia coli by Biobrick Method

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