Multiple genes control particulate phosphofructokinase of yeast

Parmar, Latika; Lobo, Zita; Nadkarni, Manasi; Maitra, P. K.

doi:10.1007/bf00329953

Cited by 8 publications

(2 citation statements)

References 6 publications

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“…Glucose-6-phosphate can also be further converted into fructose-6-phosphate by the phosphoglucose isomerases Pgi1 and Cdc30 (Dickinson and Williams, 1987; Dickinson, 1991). Fructose-6-phosphate is then converted into fructose-1,6-bisphosphate by phosphofructokinases Pfk1 and Pkf2 (Lobo and Maitra, 1983; Parmar et al, 1984). Importantly, recent work revealed that fructose-1,6-bisphosphate is the key product that connects glycolysis to the cAMP/PKA pathway.…”

Section: Mechanisms Of Nutrient Sensing Mediated By the Camp/pka Pathmentioning

confidence: 99%

The cAMP/Protein Kinase A Pathway Regulates Virulence and Adaptation to Host Conditions in Cryptococcus neoformans

Caza

Kronstad

2019

Front. Cell. Infect. Microbiol.

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Nutrient sensing is critical for adaptation of fungi to environmental and host conditions. The conserved cAMP/PKA signaling pathway contributes to adaptation by sensing the availability of key nutrients such as glucose and directing changes in gene expression and metabolism. Interestingly, the cAMP/PKA pathway in fungal pathogens also influences the expression of virulence determinants in response to nutritional and host signals. For instance, protein kinase A (PKA) in the human pathogen Cryptococcus neoformans plays a central role in orchestrating phenotypic changes, such as capsule elaboration and melanin production, that directly impact disease development. In this review, we focus first on insights into the role of the cAMP/PKA pathway in nutrient sensing for the model yeast Saccharomyces cerevisiae to provide a foundation for understanding the pathway in C. neoformans . We then discuss key features of cAMP/PKA signaling in C. neoformans including new insights emerging from the analysis of transcriptional and proteomic changes in strains with altered PKA activity and expression. Finally, we highlight recent studies that connect the cAMP/PKA pathway to cell surface remodeling and the formation of titan cells.

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Section: Mechanisms Of Nutrient Sensing Mediated By the Camp/pka Pathmentioning

confidence: 99%

The cAMP/Protein Kinase A Pathway Regulates Virulence and Adaptation to Host Conditions in Cryptococcus neoformans

Caza

Kronstad

2019

Front. Cell. Infect. Microbiol.

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“…Met6 is a cobalamin-independent methionine synthase that is involved in methionine biosynthesis and regeneration [9]. Pfk2 is the beta subunit of phosphofructokinase that partakes in glycolysis [10]. Ura2 is bifunctional enzyme demonstrating both carbamoylphosphate synthetase and aspartate transcarbamylase activity, which catalyzes the first two enzymatic steps in pyrimidine biosynthesis [11].…”

Section: Introductionmentioning

confidence: 99%

A Triple Knockout (TKO) Proteomics Standard for Diagnosing Ion Interference in Isobaric Labeling Experiments

Paulo

O’Connell

Gygi

2016

J. Am. Soc. Mass Spectrom.

164

214

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Isobaric labeling is a powerful strategy for quantitative mass spectrometry-based proteomic investigations. A complication of such analyses has been the co-isolation of multiple analytes of similar mass-to-charge resulting in the distortion of relative protein abundance measurements across samples. When properly implemented, triple-stage mass spectrometry and synchronous precursor selection (SPS-MS3) can reduce the occurrence of this phenomena, referred to as ion interference. However, no diagnostic tool is available currently to rapidly and accurately assess ion interference. To address this need, we developed a multiplexed TMT-based standard, termed the triple knockout (TKO). This standard is comprised of three yeast proteomes in triplicate, each from a strain deficient in a highly abundant protein (Met6, Pfk2, or Ura2). The relative abundance patterns of these proteins, which can be inferred from dozens of peptide measurements, are representative of ion interference in peptide quantification. We expect no signal in channels where the protein is knocked out, permitting maximum sensitivity for measurements of ion interference against a null background. Here, we emphasize the need to investigate further ion interference-generated ratio distortion and promote the TKO standard as a tool to investigate such issues.

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Is the phosphofructokinase‐reaction obligatory for glucose fermentation by Saccharomyces cerevisiae?

Heinisch¹,

Zimmerman²

1985

Yeast

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Multiple genes control particulate phosphofructokinase of yeast

Abstract: Mutants of Saccharomyces cerevisiae lacking the particulate phosphofructokinase define at least four unlinked genes, PFK2, PFK3, PFK4 and PFK5. A structural role of PFK2 is indicated. Mutations in the other three have pleiotropic effects.

Cited by 8 publications

References 6 publications

The cAMP/Protein Kinase A Pathway Regulates Virulence and Adaptation to Host Conditions in Cryptococcus neoformans

The cAMP/Protein Kinase A Pathway Regulates Virulence and Adaptation to Host Conditions in Cryptococcus neoformans

A Triple Knockout (TKO) Proteomics Standard for Diagnosing Ion Interference in Isobaric Labeling Experiments

Is the phosphofructokinase‐reaction obligatory for glucose fermentation by Saccharomyces cerevisiae?

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