Multiple Genetic Typing of
            <i>Salmonella enterica</i>
            Serotype Typhimurium Isolates of Different Phage Types (DT104, U302, DT204b, and DT49) from Animals and Humans in England, Wales, and Northern Ireland

Liébana, Ernesto; Migura-García, Lourdes; Clouting, C.; Clifton-Hadley, F. A.; Lindsay, Elisabeth; Threlfall, E. J.; McDowell, S.W.J.; Davies, Robert

doi:10.1128/jcm.40.12.4450-4456.2002

Cited by 53 publications

(51 citation statements)

References 30 publications

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“…The same serovars plus S. Senftenberg were isolated by Saha et al (2001) from faecal samples taken from hospitalized diarrhoeal children in India. Similar to this study, Liebana et al (2001Liebana et al ( , 2002and Tamada et al (2001) reported the isolation of S. Mbandaka, S. Montevideo and S. Livingstone from animal sources. The findings of Delicato et al (2004) and of Fernandez et al (2003) were also comparable to our results.…”

Section: Discussionsupporting

confidence: 71%

Prevalence, Detection and Antimicrobial Resistance Pattern of Salmonella in Sudan

Hussein¹,

Mohy-Eldin²,

Elmadiena³

et al. 2012

Salmonella - Distribution, Adaptation, Control Measures and Molecular Technologies

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Section: Discussionsupporting

confidence: 71%

Prevalence, Detection and Antimicrobial Resistance Pattern of Salmonella in Sudan

Hussein¹,

Mohy-Eldin²,

Elmadiena³

et al. 2012

Salmonella - Distribution, Adaptation, Control Measures and Molecular Technologies

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“…A MDR S. Typhimurium strain, with definitive phage type 104 (DT 104), has emerged and spread over the world since 1984 (Leegaard et al 2000, Liebana et al 2002, Van Duijkeren et al 2003. The dominant resistance type of these strains is ACSSuT, that is, they are resistant to ampicillin, chloramphenicol, streptomycin, sulfonamides, and tetracycline, and the resistance is encoded by chromosomally located genes (Davis et al 1999, Izumiya et al 2001.…”

Section: Abstract: Salmonella Typhimurium -Phage Types -Multidrug-rementioning

confidence: 99%

“…The extensive use of antimicrobials in human and veterinary medicine has led to an increase in multidrug resistance strains (MDR) (Threfall et al 1993, Glynn et al 1998, Davis et al 1999, Rabatsky et al 2004. Fernandes et al (1992) of S. Typhimurium strains isolated from humans presented high levels of resistance to antimicrobial agents (5-14 marks) and most were isolated in hospital environments, which have been considered the principal vehicle of epidemic MDR S. Typhimurium strains (Riley et al 1984, Fernandes et al 1992, Asensi et al 1995, Kariuky et al 1999.A MDR S. Typhimurium strain, with definitive phage type 104 (DT 104), has emerged and spread over the world since 1984 (Leegaard et al 2000, Liebana et al 2002, Van Duijkeren et al 2003. The dominant resistance type of these strains is ACSSuT, that is, they are resistant to ampicillin, chloramphenicol, streptomycin, sulfonamides, and tetracycline, and the resistance is encoded by chromosomally located genes (Davis et al 1999, Izumiya et al 2001.…”

mentioning

confidence: 99%

Antimicrobial susceptibility, phage types, and pulsetypes of Salmonella Typhimurium, in São Paulo, Brazil

Ghilardi

Tavechio

Fernandes

2006

Mem. Inst. Oswaldo Cruz

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A total of 283 Salmonella Typhimurium strains isolated from cases of human infections and non human sources, were examined for antimicrobial susceptibility and the incidence of resistance was 38% and multiple resistance (to three or more antimicrobials) was 15%. All 43 multidrug-resistant strains (MDR) and 13 susceptible ones were characterized by phage typing and pulsed-field gel electrophoresis (PFGE). The strains encompassed 14 definitive phage types (DT), three were untypable (UT), and 18 atypicals or reaction does not conform (RDNC), which belonged to 21 PFGE patterns, A1-A21. The predominant phage types were DT49, DT193, and RDNC and two strains belonging to DT 104 and 104b were also identified. The most commum PFGE patterns were A1 and A8. Analysis by PFGE and phage typing demonstrated that the most of the MDR were multiclonal and association among multiresistance, phage typing, and PFGE patterns was not so significant. Key words: Salmonella Typhimurium -phage types -multidrug-resistantSalmonella is one of the most important causal agents of foodborne illness. It can be isolated from a variety of foodstuff such as raw meats, poultry and poultry products, milk and milk products (Gorman & Adley 2004). The zoonotic nature of Salmonella infections is well established and the observed increase in human cases has been associated with high levels of infections in animal husbandry, mainly in pigs and poultry (Seyfarth et al. 1997).The Salmonella genus comprises more than 2500 serotypes (Popoff 2001), most of which are considered potential human pathogens, but only a reduced number of them have been associated with human infections (Uzzau et al. 2000). In developed countries, outbreaks of nontyphoidal Salmonella infections have been caused mainly by serotypes Enteritidis and Typhimurium (Seyfarth et al. 1997, Davis et al. 1999, Kariuky et al. 1999.S. Typhimurium is a common cause of human salmonellosis in many countries , Leegaard et al. 2000, Esaki et al. 2004, Martinez-Urtaza et al. 2004, Gorman & Adley 2004. It represents the most common serotype isolated from humans and animals in the United States (Bender et al. 2001, Rabatsky et al. 2004) and the second in the United Kingdom (Martinez-Urtaza et al. 2004).Studies reported in the state of São Paulo, Brazil, have also indicated that S. Typhimurium is the second most common serotype isolated from human sources (Tavechio et al.1996) and one of the most frequent from non-human sources (Taunay et al. 1996). The extensive use of antimicrobials in human and veterinary medicine has led to an increase in multidrug resistance strains (MDR) (Threfall et al. 1993, Glynn et al. 1998, Davis et al. 1999, Rabatsky et al. 2004. Fernandes et al. (1992) of S. Typhimurium strains isolated from humans presented high levels of resistance to antimicrobial agents (5-14 marks) and most were isolated in hospital environments, which have been considered the principal vehicle of epidemic MDR S. Typhimurium strains (Riley et al. 1984, Fernandes et al. 1992, Asensi et al. 1995, Kariuky...

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“…Over the past two decades genotyping has been associated to the traditional typing methods to achieve a better discrimination of strains and to identify bacterial clones (28). Among the molecular methods, pulsed-field gel electrophoresis (PFGE) is considered the standard method for DNA fingerprinting in Salmonella, and has been performed to investigate salmonellosis outbreaks (11,16,17,21,40).…”

Section: Introductionmentioning

confidence: 99%

Quantification and molecular characterization of Salmonella isolated from food samples involved in salmonellosis outbreaks in Rio Grande do Sul, Brazil

Mürmann

Santos

Longaray³

et al. 2008

Braz. J. Microbiol.

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Data concerning the prevalence and populations of Salmonella in foods implicated in outbreaks may be important to the development of quantitative microbial risk assessments of individual food products. In this sense, the objective of the present study was to assess the amount of Salmonella sp. in different foods implicated in foodborne outbreaks in Rio Grande do Sul occurred in 2005 and to characterize the isolated strains using phenotypic and genotypic methods. Nineteen food samples involved in ten foodborne outbreaks occurred in 2005, and positive on Salmonella isolation at the Central Laboratory of the Health Department of the State of Rio Grande do Sul, were included in this study. Food samples were submitted to estimation of Salmonella using the Most Probable Number (MPN) technique. Moreover, one confirmed Salmonella colony of each food sample was serotyped, characterized by its XbaI-macrorestriction profile, and submitted to antimicrobial resistance testing. Foods containing eggs, mayonnaise or chicken were contaminated with Salmonella in eight outbreaks. Higher counts (>10 7 MPN.g -1) of Salmonella were detected mostly in foods containing mayonnaise. The isolation of Salmonella from multiple food items in five outbreaks probably resulted from the cross-contamination, and the high Salmonella counts detected in almost all analyzed samples probably resulted from storing in inadequate temperature. All strains were identified as S. Enteritidis, and presented a unique macrorestriction profile, demonstrating the predominance of one clonal group in foods involved in the salmonellosis outbreaks. A low frequency of antimicrobial resistant S. Enteritidis strains was observed and nalidixic acid was the only resistance marker detected.

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Multiple Genetic Typing of Salmonella enterica Serotype Typhimurium Isolates of Different Phage Types (DT104, U302, DT204b, and DT49) from Animals and Humans in England, Wales, and Northern Ireland

Cited by 53 publications

References 30 publications

Prevalence, Detection and Antimicrobial Resistance Pattern of Salmonella in Sudan

Prevalence, Detection and Antimicrobial Resistance Pattern of Salmonella in Sudan

Antimicrobial susceptibility, phage types, and pulsetypes of Salmonella Typhimurium, in São Paulo, Brazil

Quantification and molecular characterization of Salmonella isolated from food samples involved in salmonellosis outbreaks in Rio Grande do Sul, Brazil

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