Multiple hormone actions: The rises in cAMP and Ca ++ in mdck-cells treated with glucagon and prostaglandin E 1 are independent processes

Kurstjens, N.; Heithier, Helmuth; Cantrill, R.C.; Hahn, Matthias; Boege, Fritz

doi:10.1016/0006-291x(90)90645-4

Cited by 14 publications

(2 citation statements)

References 18 publications

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“…On the other hand, glucagon and prostaglandin El receptors on MDCK cells are activated by their respective agonists, apparently in about the same range of concentrations, but each appears to be responsible for a concomitant rise in intracellular Ca2+ and CAMP, suggesting that a single receptor may activate more than one G-protein (Kurstjens et al 1990). This was first demonstrated in reconstitution experiments where it was shown that rhodopsin stimulated not only GTP hydrolysis by transducin, but also by a mixture of human erythrocyte Gi-2 and Gi-3 proteins (Cerione et al, 1985).…”

Section: Implications Of Structural Heterogeneity Of Receptors and G-mentioning

confidence: 99%

Structural heterogeneity of membrane receptors and GTP-binding proteins and its functional consequences for signal transduction

1991

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Recent information obtained, mainly by recombinant cDNA technology, on structural heterogeneity of hormone and transmitter receptors, of GTP-binding proteins (G-proteins) and, especially, of G-protein-linked receptors is reviewed and the implications of structural heterogeneity for diversity of hormone and transmitter actions is discussed. For the future, three-dimensional structural analysis of membrane proteins participating in signal transmission and transduction pathways is needed in order to understand the molecular basis of allosteric regulatory mechanisms governing the interactions between these proteins including hysteretic properties and cellcybernetic aspects.Thanks to recombinant DNA technology, there has been a recent flood of structural information on membrane receptors, G-proteins and target enzymes [for reviews see Gilman

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Section: Implications Of Structural Heterogeneity Of Receptors and G-mentioning

confidence: 99%

Structural heterogeneity of membrane receptors and GTP-binding proteins and its functional consequences for signal transduction

1991

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“…7B also shows that anacardic acid, a histone acetylase inhibitor, also had an inhibitory effect on the PGE 1 stimulation. 1 Response-PGE 1 has been observed to cause an increase in intracellular calcium levels as well as intracellular cAMP levels in MDCK cells (27,28). Thus, PGE 1 may initiate signaling through PKC and/or Ca 2ϩ /calmodulin-dependent protein kinase (CaM kinase) as well as cAMP-dependent protein kinase.…”

Section: Fig 7 Role Of Creb and Sp1 In Mediating Pge 1 Stimulationmentioning

confidence: 99%

Identification of a Prostaglandin-responsive Element in the Na,K-ATPase β1 Promoter That Is Regulated by cAMP and Ca2+

Matlhagela

Borsick

Rajkhowa

et al. 2005

Journal of Biological Chemistry

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The Na,K-ATPase is a transmembrane protein responsible for maintaining electrochemical gradients across the plasma membrane in all mammalian cells, a process that is subject to regulation at the transcriptional as well as post-transcriptional level. Included among physiologic regulators in the kidney are prostaglandins. Previously, we demonstrated that prostaglandin E 1 (PGE 1 ) increases the activity and expression of the Na,KATPase in Madin-Darby canine kidney cells (Taub, M., Borsick, M., Geisel, J., Matlhagela, K., Rajkhowa, T., and In this work, we present evidence that transcription of the Na,K-ATPase ␤ 1 subunit is stimulated by PGE 1 , an effect that may be mediated through the cAMP and Ca 2؉ pathways. Transient transfection studies using 5-deletion mutants of the human ␤ 1 subunit promoter indicated that region ؊100 to ؊92 containing the sequence AGTCCCTGC (a prostaglandin-responsive element (PGRE)) is required to elicit the stimulatory effects of PGE 1 , 8-bromo-cAMP, phorbol 12-myristate 13-acetate, and okadaic acid. Electrophoretic mobility shift assays indicated that both the cAMP regulatory element-binding protein (CREB) and Sp1 bind to the PGRE present within this region of the ␤ 1 subunit promoter. The involvement of the PGRE and Sp1 sites in regulation by PGE 1 was further confirmed by the increased PGE 1 stimulation that was observed following insertion of the PGRE into a promoter/luciferase construct containing a portion of a heterologous promoter and the fibronectin promoter with four GC boxes. Further evidence suggesting an interaction between Sp1 and CREB was obtained from experiments conducted with pLuc-MCS-␤72-167, which contains region ؊167 to ؊72 in the human ␤ 1 subunit promoter. The PGE 1 stimulation observed in Madin-Darby canine kidney cells transiently transfected with pLuc-MCS-␤72-167 was reduced when the two GC boxes immediately upstream from the PGRE were translocated farther upstream. Also consistent with an interaction between CREB and Sp1 are the results of our immunoprecipitation studies indicating that CREB co-immunoprecipitated with Sp1 when an antibody against CREB, Sp1, or the CREB-binding protein was used.

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Regulation of the Na, K‐ATPase activity of Madin‐Darby Canine Kidney cells in defined medium by Prostaglandin E₁ and 8‐bromocyclic AMP

Taub

Wang

IlSuk

et al. 1992

Journal Cellular Physiology

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The role of PGE1 in regulating the activity of the Na+, K(+)-ATPase in Madin Darby Canine Kidney (MDCK) cells has been examined. PGE1 increased the initial rate of ouabain-sensitive Rb+ uptake by MDCK cells, a process that continued to occur over a 5-day period. The increase in the initial rate of ouabain-sensitive Rb+ uptake in MDCK cells treated with PGE1 could be explained by a 1.6-fold increase in the Vmax for ouabain-sensitive Rb+ uptake. The increase in the Vmax for ouabain-sensitive Rb+ uptake observed in MDCK cells under these conditions can be explained either by an increase in the number of active Na+ pumps, or by an increase in the efficiency of the Na+ pumps. Consistent with the former possibility is the observed increase in the number of ouabain binding sites, as well as the increase in Na+, K(+)-ATPase activity in cell lysates obtained from MDCK monolayers treated with PGE1. The involvement of cyclic AMP in mediating these effects of PGE1 on the Na+, K(+)-ATPase in MDCK cells is supported by: (1) the observation of similar effects in 8-bromocyclic AMP treated MDCK monolayers, and (2) a dramatic reduction of the stimulatory effects of PGE1 and 8-bromocyclic AMP on the Vmax for ouabain-sensitive Rb+ uptake, and on the number of ouabain binding sites in dibutyryl cyclic AMP resistant clone 3 (DBr3) (which is defective in cyclic AMP dependent protein kinase activity). PGE1 independent MDCK monolayers exhibit both an increase in the Vmax for ouabain-sensitive Rb+ uptake and an increase in the number of ouabain binding sites in response to 8-bromocyclic AMP. Apparently, the cyclic AMP phosphodiesterase defect in these PGE1 independent cells did not cause cellular cyclic AMP levels to be elevated to a sufficient extent to maximally increase the Na+, K(+)-ATPase activity in these variant cells.

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Multiple hormone actions: The rises in cAMP and Ca ++ in mdck-cells treated with glucagon and prostaglandin E 1 are independent processes

Cited by 14 publications

References 18 publications

Structural heterogeneity of membrane receptors and GTP-binding proteins and its functional consequences for signal transduction

Structural heterogeneity of membrane receptors and GTP-binding proteins and its functional consequences for signal transduction

Identification of a Prostaglandin-responsive Element in the Na,K-ATPase β1 Promoter That Is Regulated by cAMP and Ca2+

Regulation of the Na, K‐ATPase activity of Madin‐Darby Canine Kidney cells in defined medium by Prostaglandin E₁ and 8‐bromocyclic AMP

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Multiple hormone actions: The rises in cAMP and Ca ++ in mdck-cells treated with glucagon and prostaglandin E 1 are independent processes

Cited by 14 publications

References 18 publications

Structural heterogeneity of membrane receptors and GTP-binding proteins and its functional consequences for signal transduction

Structural heterogeneity of membrane receptors and GTP-binding proteins and its functional consequences for signal transduction

Identification of a Prostaglandin-responsive Element in the Na,K-ATPase β1 Promoter That Is Regulated by cAMP and Ca2+

Regulation of the Na, K‐ATPase activity of Madin‐Darby Canine Kidney cells in defined medium by Prostaglandin E1 and 8‐bromocyclic AMP

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Regulation of the Na, K‐ATPase activity of Madin‐Darby Canine Kidney cells in defined medium by Prostaglandin E₁ and 8‐bromocyclic AMP