Multiple Independent Defective <i>Suppressor-mutator</i> Transposon Insertions in Arabidopsis: A Tool for Functional Genomics

Tissier, Alain; Marillonnet, Sylvestre; Klimyuk, Victor; Patel, Kanu; Torres, Miguel Ángel Medina; Murphy, George P.; Jones, Jonathan D. G.

doi:10.1105/tpc.11.10.1841

Cited by 341 publications

(301 citation statements)

References 45 publications

Supporting

Mentioning

296

Contrasting

Unclassified

Order By: Relevance

“…Sequence-indexed SALK [18] and GARLIC (now referred to as SAIL) [19] T-DNA insertion lines were obtained from the Nottingham Arabidopsis Stock Centre (NASC; http://arabidopsis.info/) and Syngenta Biotechnology, respectively. SLAT transposon insertion lines [20] were selected by PCR using pooled DNA samples and appropriate oligonucleotides. Mature pollen grains from $400 plants per genotype were harvested using a vacuum system adapted from Johnson-Brousseau and McCormick [21], and stocked at À20°C until protein extraction (see below).…”

Section: Methodsmentioning

confidence: 99%

A reference map of the Arabidopsis thaliana mature pollen proteome

Noir

Bräutigam

Colby

et al. 2005

Biochemical and Biophysical Research Communications

137

145

View full text Add to dashboard Cite

The male gametophyte (or pollen) plays an obligatory role during sexual reproduction of higher plants. The extremely reduced complexity of this organ renders pollen a valuable experimental system for studying fundamental aspects of plant biology such as cell fate determination, cell-cell interactions, cell polarity, and tip-growth. Here, we present the first reference map of the mature pollen proteome of the dicotyledonous model plant species, Arabidopsis thaliana. Based on two-dimensional gel electrophoresis, matrix-assisted laser desorption/ionization time-of-flight, and electrospray quadrupole time-of-flight mass spectrometry, we reproducibly identified 121 different proteins in 145 individual spots. The presence, subcellular localization, and functional classification of the identified proteins are discussed in relation to the pollen transcriptome and the full protein complement encoded by the nuclear Arabidopsis genome. Ó 2005 Elsevier Inc. All rights reserved.Keywords: Arabidopsis thaliana; Male gametophyte; Mass spectrometry; Mature pollen; Proteome; Two-dimensional gel electrophoresis In higher plants, development of the male gametophyte is a well-programmed and elaborate process. Male sporogenesis begins with the division of a diploid sporophytic cell giving rise to the anther wall of the stamen and the sporogenic cells. The latter cells then undergo several mitoses to differentiate into pollen mother cells. Subsequently, each diploid pollen mother cell forms a tetrad of haploid microspores via a round of DNA replication followed by two consecutive meiotic divisions. Each uninucleate microspore then undergoes an asymmetric mitotic division forming a large vegetative cell and a smaller generative cell (i.e., the bicellular pollen stage). In Arabidopsis, the generative cell undergoes another mitotic division giving rise to two sperm cells (i.e., the tricellular pollen stage). Following pollination, the vegetative cell controls the further development of the mature pollen grain and growth of the pollen tube into the style until both sperm cell nuclei are delivered to the embryo sac in the ovule, where they participate in double fertilization [1,2].The last two decades have been marked by increasing efforts to decipher the genetic and molecular basis of pollen development and functions [reviewed in 1,3,4]. In the model plant Arabidopsis thaliana, the extremely reduced, tricellular male gametophyte constitutes an ideal experimental system for analyses of important biological processes in higher plant reproduction. In addition, it represents a very useful model for studying fundamental aspects of plant biology such as cell fate determination, cell-cell interactions, cell polarity, and tip-growth [5][6][7].The availability of the full genome sequence of Arabidopsis [8] has made genome-wide, microarray-based analyses of the male gametophyte transcriptome of this model plant possible [9][10][11][12]. 13,977 male gametophyte-expressed mRNAs were recently identified using the ATH1 Genome Array, which cover...

show abstract

Section: Methodsmentioning

confidence: 99%

A reference map of the Arabidopsis thaliana mature pollen proteome

Noir

Bräutigam

Colby

et al. 2005

Biochemical and Biophysical Research Communications

137

145

View full text Add to dashboard Cite

show abstract

“…Subsequently, the laboratory participated in generating the Garlic lines in collaboration with the former Torrey Mesa Research Institute, and one additional line was isolated (Sessions et al, 2002). A dSpm insertion line for IAA5 was also identified (Tissier et al, 1999). Taken together during the last 6 years, we identified 13 T-DNA insertion lines located between the start and stop codons of 12 Aux/IAA genes.…”

Section: Isolation Of Aux/iaa T-dna Insertion Mutantsmentioning

confidence: 99%

Functional Genomic Analysis of theAUXIN/INDOLE-3-ACETIC ACIDGene Family Members inArabidopsis thaliana [W]

et al. 2005

View full text Add to dashboard Cite

Auxin regulates various aspects of plant growth and development. The AUXIN/INDOLE-3-ACETIC ACID (Aux/IAA) genes encode short-lived transcriptional repressors that are targeted by the TRANSPORT INHIBITOR RESPONSE1/AUXIN RECEPTOR F-BOX proteins. The Aux/IAA proteins regulate auxin-mediated gene expression by interacting with members of the AUXIN RESPONSE FACTOR protein family. Aux/IAA function is poorly understood; herein, we report the identification and characterization of insertion mutants in 12 of the 29 Aux/IAA family members. The mutants show no visible developmental defects compared with the wild type. Double or triple mutants of closely related Aux/IAA genes, such as iaa8-1 iaa9-1 or iaa5-1 iaa6-1 iaa19-1, also exhibit wild-type phenotypes. Global gene expression analysis reveals that the molecular phenotypes of auxin-treated and untreated light-grown seedlings are unaffected in the iaa17-6 and iaa5-1 iaa6-1 iaa19-1 mutants. By contrast, similar analysis with the gain-of-function axr3-1/iaa17-1 mutant seedlings reveals dramatic changes in basal and auxin-induced gene expression compared with the wild type. Expression of several type-A ARABIDOPSIS RESPONSE REGULATOR genes and a number of genes involved in cell wall biosynthesis and degradation is repressed in axr3-1/iaa17-1. The data suggest extensive functional redundancy among Aux/IAA gene family members and that enhanced stability of the AXR3/IAA17 protein severely alters the molecular phenotype, resulting in developmental defects.

show abstract

“…Individual spl and cyp78a T-DNA insertion lines (Tissier et al, 1999;Sessions et al, 2002;Alonso et al, 2003) were obtained from the European Arabidopsis Stock Centre (http://Arabidopsis.info). The cyp78a5 allele has also been described as klu-4 (Anastasiou et al, 2007).…”

Section: Plant Materialsmentioning

confidence: 99%

Dual Effects of miR156-TargetedSPLGenes andCYP78A5/KLUHon Plastochron Length and Organ Size inArabidopsis thaliana

et al. 2008

View full text Add to dashboard Cite

Leaves of flowering plants are produced from the shoot apical meristem at regular intervals, with the time that elapses between the formation of two successive leaf primordia defining the plastochron. We have identified two genetic axes affecting plastochron length in Arabidopsis thaliana. One involves microRNA156 (miR156), which targets a series of SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) genes. In situ hybridization studies and misexpression experiments demonstrate that miR156 is a quantitative, rather than spatial, modulator of SPL expression in leaf primordia and that SPL activity nonautonomously inhibits initiation of new leaves at the shoot apical meristem. The second axis is exemplified by a redundantly acting pair of cytochrome P450 genes, CYP78A5/KLUH and CYP78A7, which are likely orthologs of PLASTOCHRON1 of rice (Oryza sativa). Inactivation of CYP78A5, which is expressed at the periphery of the shoot apical meristem, accelerates the leaf initiation rate, whereas cyp78a5 cyp78a7 double mutants often die as embryos with supernumerary cotyledon primordia. The effects of both miR156-targeted SPL genes and CYP78A5 on organ size are correlated with changes in plastochron length, suggesting a potential compensatory mechanism that links the rate at which leaves are produced to final leaf size.

show abstract

Multiple Independent Defective Suppressor-mutator Transposon Insertions in Arabidopsis: A Tool for Functional Genomics

Cited by 341 publications

References 45 publications

A reference map of the Arabidopsis thaliana mature pollen proteome

A reference map of the Arabidopsis thaliana mature pollen proteome

Functional Genomic Analysis of theAUXIN/INDOLE-3-ACETIC ACIDGene Family Members inArabidopsis thaliana [W]

Dual Effects of miR156-TargetedSPLGenes andCYP78A5/KLUHon Plastochron Length and Organ Size inArabidopsis thaliana

Contact Info

Product

Resources

About