Multiple micromanipulations for preimplantation genetic diagnosis do not affect embryo development to the blastocyst stage

Substandard application of preimplantation genetic screening may interfere with its clinical success

“…The true effect of biopsy therefore is still unknown. However, in experienced centers, polar body, blastomere, or a combination of polar body and biopsy does not affect pregnancy rates negatively (14)(15)(16). In addition, indirect evidence concerning the effect of biopsy on embryo viability was obtained in studies assessing the loss of cells from 5-to 10-cell embryos after freezing and thawing.…”

Mesenchymal Stem Cells: Possibilities of New Treatment Options

“…When the embryo develops to the six-to eight-blastomere stage (day 3), one blastomere biopsy is done. It has not been shown that polar body removal has an additional negative affect on embryo survival compared to blastomere biopsy (Cieslak et al 2006 ) . Single blastomere biopsy is thought to reduce the pregnancy rate nearly 10%, and biopsy of two blastomere cells may cause a low pregnancy rate (Cohen et al 2007 ) .…”

The Role of Aneuploidy Screening in Human Preimplantation Embryos

“…Sometimes up to four manipulations -ICSI, PB1, PB2 and blastomere biopsy (as a reflexive test following test failure or an ambiguous PB result) may be required. However, in experienced hands, even 3 independent biopsy manipulations appear to have no deleterious effect on development (Magli et al 2004;Cieslak-Janzen et al 2006). Although simultaneous removal of PB1 and PB2 is possible on day 1 of embryo development there may be advantages to sequential biopsy where PB1 is removed on day 0 (day of insemination) followed by the removal of PB2 on day 1.…”