Multiple olfactory pathways contribute to the lure process of Caenorhabditis elegans by pathogenic bacteria

Zhu, Man; Yao, Chi; Zhao, Ninghui; Bai, Hua; Zhang, Ke‐Qin; Huang, Xiaowei

doi:10.1007/s11427-020-1842-7

Cited by 7 publications

(8 citation statements)

References 40 publications

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“…Both AWA and AWC olfactory neurons are responsible for detecting FAc odors. We therefore hypothesized that the GPCRs ODR-10 and STR-2, expressed in AWA and AWC neurons, respectively ( 16 – 19 , 23 ), and SRA-13, expressed in both AWA and AWC neurons ( 16 ), are likely receptors for FAc. Chemotaxis assays revealed that worms with a mutation in str-2 ( ok3148 ) or sra-13 ( zh13 ) were defective in sensing FAc, but nematodes with the odr-10 ( ky32 ) mutation retained a normal response to FAc ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The highly developed chemosensory system of C. elegans comprises 12 pairs of amphid chemosensory neurons, which enable it to detect a wide variety of signals associated with food, danger, or other animals ( 16 , 17 ). The detection of attractive volatile organic compounds (VOCs) is mediated mainly by the AWA and AWC olfactory neurons ( 16 – 19 ). Most chemosensory odorant receptors in C. elegans are G-protein-coupled receptors (GPCRs) ( 20 ).…”

Section: Introductionmentioning

confidence: 99%

“…Most chemosensory odorant receptors in C. elegans are G-protein-coupled receptors (GPCRs) ( 20 ). The genome of C. elegans is predicted to harbor at least 1,300 GPCR genes ( 21 ), accounting for approximately 7% of its protein-encoding genes ( 22 ), but only a few GPCRs of olfactory neurons have known specific ligands ( 18 – 20 , 23 ). The individual sensory receptors that respond to ligands and the subsequent signaling cascades that they initiate in the olfactory neurons of C. elegans still require further elucidation.…”

Section: Introductionmentioning

confidence: 99%

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Multiple Receptors Contribute to the Attractive Response of Caenorhabditis elegans to Pathogenic Bacteria

et al. 2023

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Multiple Receptors Contribute to the Attractive Response of Caenorhabditis elegans to Pathogenic Bacteria

et al. 2023

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“…In addition, some microorganisms with biocontrol potential secrete small molecular compounds to attract nematodes and further kill them. For example, Bacillus nematocida B16, which has good application prospects, produces 2-heptanone to attract nematodes, subsequently infesting and killing them through protease secretion [ 43 , 44 ], while Arthrobotrys oligospora produces volatile methyl 3-methyl-2-butenoate to attract C. elegans and realizes a nematode trapping strategy [ 45 ]. Two Zn(2)-C6 transcription factors responsible for the regulation of fungal metabolism were significantly upregulated during the interaction between P. lavendulum and C. elegans .…”

Section: Discussionmentioning

confidence: 99%

Identification of Nematicidal Metabolites from Purpureocillium lavendulum

Bao

et al. 2022

Microorganisms

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Purpureocillium lavendulum is a fungus with promising biocontrol applications. Here, transcriptome data acquired during the infection of Caenorhabditis elegans by Purpureocillium lavendulum showed that the transcription of metabolite synthesis genes was significantly up-regulated after 24 and 48 h of the fungus-nematode interaction. Then, the up-regulated transcription level of lipoxygenase was confirmed by RT-qPCR. The ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) analysis of differential metabolites revealed that this interaction resulted in the emergence of new metabolites or enhanced the production of metabolites. The results of the UPLC-MS analysis and the nematicidal assay were used to establish optimal culturing conditions under which 12 metabolites, including 3 hydroxylated C18 fatty acids and 9 steroids, were isolated and identified. Among them, hydroxylated fatty acids showed pronounced nematicidal activity against Meloidogyne incognita, and two degradative sterols showed chemotaxis activity to M. incognita. This study lays a foundation for the function of lipoxygenase and its products during the infection of Purpureocillium lavendulum.

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“…Previous studies had shown that Bacillus and Pseudomonas spp. were the most common populations that colonized the rhizosphere and could effectively antagonize root-knot nematodes for their nematocidal activity ( Zhu et al, 2021 ; Antil et al, 2022 ; Gowda et al, 2022 ). Here, the repulsive response of M. incognita against two species of biocontrol bacteria, including B. nematocida B16 and B. thuringiensis Bt79, was investigated and the signal pathways underlying this behavior were elucidated.…”

Section: Introductionmentioning

confidence: 99%

Repulsive response of Meloidogyne incognita induced by biocontrol bacteria and its effect on interspecific interactions

et al. 2022

Self Cite

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The aversive behavior of Caenorhabditis elegans is an important strategy that increases their survival under pathogen infection, and the molecular mechanisms underlying this behavior have been described. However, whether this defensive response occurs in plant-parasitic nematodes (PPNs), which have quite different life cycles and genomic sequences from the model nematode, against biocontrol microbes and affects interspecific interactions in ecological environments remains unclear. Here, we showed that Meloidogyne incognita, one of the most common PPNs, engaged in lawn-leaving behavior in response to biocontrol bacteria such as Bacillus nematocida B16 and B. thuringiensis Bt79. Genomic analysis revealed that the key genes responsible for the aversive behavior of C. elegans, such as serotonin-and TGF-β-related genes in canonical signaling pathways, were homologous to those of M. incognita, and the similarity between these sequences ranged from 30% to 67%. Knockdown of the homologous genes impaired avoidance of M. incognita to varying degrees. Calcium ion imaging showed that the repulsive response requires the involvement of the multiple amphid neurons of M. incognita. In situ hybridization specifically localized Mi-tph-1 of the serotonin pathway to ADF/ NSM neurons and Mi-dbl-1 of the TGF-β pathway to AVA neurons. Our data suggested that the repulsive response induced by different biocontrol bacteria strongly suppresses the invasion of tomato host plants by M. incognita. Overall, our study is the first to clarify the pathogen-induced repulsive response of M. incognita and elucidate its underlying molecular mechanisms. Our findings provide new insights into interspecific interactions among biocontrol bacteria, PPNs, and host plants.

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Multiple olfactory pathways contribute to the lure process of Caenorhabditis elegans by pathogenic bacteria

Cited by 7 publications

References 40 publications

Multiple Receptors Contribute to the Attractive Response of Caenorhabditis elegans to Pathogenic Bacteria

Multiple Receptors Contribute to the Attractive Response of Caenorhabditis elegans to Pathogenic Bacteria

Identification of Nematicidal Metabolites from Purpureocillium lavendulum

Repulsive response of Meloidogyne incognita induced by biocontrol bacteria and its effect on interspecific interactions

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