Multiple origins of the western European house mouse in the Aeolian Archipelago: clues from mtDNA and chromosomes

Abstract

The expansion of Mus musculus domesticus from its origin has been studied in detail. The colonization routes and times depended on its commensal habits which favoured a rapid and recent dispersal, making it difficult to unravel the expansion pattern. The situation is still obscure in the central Mediterranean area. Mitochondrial D-loop was sequenced for 65 mice from the Aeolian Archipelago and the sixteen haplotypes identified were compared with the 528 available mouse haplotypes. The central Mediterranean phylogeography, the demographic history of the Aeolian mice and the relationships between mtDNA and karyotypes was investigate. Five lineages are present, belonging to five of the haplogroups previously described for the Mediterranean basin, and most individuals fall within the European haplogroups. The Archipelago was subjected to multiple colonizations and chromosomal and molecular data agree in indicating Sicily and Italy as possible sources of colonization in recent times. Nevertheless, the signatures of earlier colonizations might have been lost through extinction and admixing of mice due to human movements. Drastic events during the entire colonization process have led to the present-day random distribution of haplotypes. Furthermore, Salina emerges as an ancestral condition and no relation between karyotype composition and haplotype variability was highlighted

Interplay between historical and current features of the cityscape in shaping the genetic structure of the house mouse (Mus musculus domesticus) in Dakar (Senegal, West Africa)

“…All 40 D-loop sequences (731 bp) from this study were aligned with 119 sequences from Senegal (including five sequences from DAK), published by Lippens et al (2017), and with 1958 sequences retrieved from GenBank: 1672 whose references were compiled in Lippens et al (2017) and 168 published in other studies (Castiglia et al, 2005;Förster et al, 2009;Gabriel et al, 2011;García-Rodríguez et al, 2018;Jones, Jóhannesdóttir, et al, 2011;Jones, Skirnisson, et al, 2012;King, 2016;MacKay et al, 2013;Solano et al, 2013;Storz et al, 2007). Sequences alignment was performed using the Clustal W multiple alignment in Bioedit v7.2.6 software (Hall, 1999).…”

Interplay between historical and current features of the cityscape in shaping the genetic structure of the house mouse (Mus musculus domesticus) in Dakar (Senegal, West Africa)

“…All 40 D-loop sequences (731 bp) from this study were aligned with 119 sequences from Senegal (including five sequences from DAK), published by Lippens et al (2017), and with 1958 sequences retrieved from GenBank: 1672 whose references were compiled in Lippens et al (2017) and 168 published in other studies (Castiglia et al, 2005;Förster et al, 2009;Gabriel et al, 2011;García-Rodríguez et al, 2018;Jones et al, 2011Jones et al, , 2012King, 2016;MacKay et al, 2013;Solano et al, 2013;Storz et al, 2007). Sequences alignment was performed using the Clustal W multiple alignment in Bioedit v7.2.6 software (Hall, 1999).…”

Mice on the borders: genetic determinations of rat and house mouse species in Lampedusa and Pantelleria islands (Southern Italy)

“…(one from Pantelleria and two from Lampedusa) have been extracted from body muscle preserved in 96% ethanol by salting out procedure (Aljanabi & Martinez, 1997). Two mitochondrial fragments were amplified for the two genera in order to produce sequences comparable to the ones available in GenBank and previous works (Solano et al, 2013). Obtained sequences were checked, edited, and aligned with Geneious v4.8.3 (Biomatter).…”

“…The 822 bp long fragments obtained are made available in GenBank (Accession numbers will be provided upon acceptance: XXX). The resulting haplotypes were combined with the dataset used by Solano et al (2013) which included 183 haplotypes from all the Mediterranean basin and northern Europe including Africa, and previous haplotypes from Pantelleria (N = 1) and Lampedusa (N = 2). Moreover, further genetic sequences available on GenBank and belonging to haplotypes from other small central Mediterranean islands (Linosa, Malta, and the seven Aeolian islands) and Sicily have also been included.…”

“…Moreover, further genetic sequences available on GenBank and belonging to haplotypes from other small central Mediterranean islands (Linosa, Malta, and the seven Aeolian islands) and Sicily have also been included. A Neighbor-net network connecting all retrieved haplotypes was obtained with Splitstree v. 4.10 (Huson and Bryant 2006) to visualize the relationship among the haplotypes observed in the frame of this work and those from previous phylogenetic/phylogeographic analyses of mouse population from Mediterranean area, and to assign the new samples to the haplogroups (Hg) identified in previous works (Bonhomme et al, 2011;Jones et al, 2011;Solano et al, 2013).…”