“…During the bacterial transport process, retention of bacteria may take place due to straining and/or attachment to solid surfaces. The extent to which bacteria are retained by straining depends on the ratio of the bacteria to porous medium grain diameters, the shape and size irregularity and surface roughness of porous media, the rate of water flow, and bacterial concentration [ Bradford and Bettahar , ; Bradford et al ., ; Díaz et al ., ; Ding et al ., ; Santos and Barros , ; Stevik et al ., ; Tufenkji et al ., ]. The attachment of bacteria is affected by a number of factors, including the surface charges of both the porous media and the bacteria, the cell surface hydrophobicity (CSH), the composition of the cell surface (e.g., lipopolysaccharide, extracellular polymeric substances, outer membrane proteins, flagella, and fimbriae), physical and geochemical properties of the solid surfaces, and groundwater chemistry (e.g., ionic strength, pH, dissolved organic matter (DOM), etc.)…”