Multiple regression analysis for assessing the growth of small hepatocellular carcinoma: The MIB-1 labeling index is the most effective parameter

Abstract

The aim of this study was to clarify whether histological parameters reflected tumor aggressiveness in patients with hepatocellular carcinoma (HCC). The tumor volume doubling times (TVDTs) of 21 HCCs, less than 3 cm in diameter at the start of the observation period, were calculated in 21 patients in whom the natural progression of the lesion was observed by ultrasonography. Paraffin-embedded sections were prepared from samples obtained by ultrasound-guided fine-needle liver biopsy at the end of the observation period. The histological parameters examined were the MIB-1 labeling index (LI), for which we performed immunohistochemical staining with the MIB-1 monoclonal antibody, using an antigen retrieval method; the nucleo-cytoplasmic (N/C ratio), cellularity, and the nuclear form factor (NFF), were calculated with an imaging analyzer. We performed multiple regression analysis for estimating the growth of small HCCs. With the N/C ratio (0.154 +/- 0.068; mean +/- SD), cellularity (453 +/- 21.8 cells/10(4) microm2), NFF (1.150 +/- 0.096), and degree of HCC differentiation as independent variables, only the MIB-1 LI (11.8 +/- 6.1%) showed a significant correlation with TVDT (207.5 +/- 162.6 days) (r = -0.658; P < 0.05). Compared to the conventional indices of histological atypism tested, i.e., N/C ratio, cellularity NFF, and degree of HCC differentiation, only MIB-1 LI was significantly correlated with small HCC growth rate. The MIB-1 LI may therefore be a simple and useful index of tumor aggressiveness.

Anticancer nanomedicine and tumor vascular permeability; Where is the missing link?

“…In addition, TDT can range depending on tumor grade. Poorly differentiated hepatocellular carcinoma is highly invasive and, predictably, has a DT of 20-78 days [128] while moderately differentiated hepatocellular carcinoma has a significantly extended DT of 94-380 days [129]. Astrocytoma also follows this trend with DT of high-grade astrocytoma at 60-78 days and the TDT of low-grade astrocytoma at 108-330 days.…”

Specific Growth Rate versus Doubling Time for Quantitative Characterization of Tumor Growth Rate

“…PC, pancreatic carcinoma (3,28). Primary lung cancers: ADN, adenocarcinoma (9,30); BAC, bronchioalveolar (30); SCC, squamous cell carcinoma (30); NSC, non-small cell carcinoma (30); NSCLC, non-small cell lung cancer (2); SCLC, small cell lung cancer (9); MLC, metastatic lung cancer from bone and soft tissue (21); HCC, hepatocellular carcinoma (27,29). W, M, and P, well, moderately, and poorly differentiated tumors, respectively.…”

The Significance of Fibroblast Growth Factor Receptor 2 Expression in Differentiation of Hepatocellular Carcinoma

“…Although poorly differentiated HCC is indicative of poor prognosis and is reported to over-express Ki-67 (proliferation-associated marker) [38], type II hexokinase (gycolytic enzyme in a hypoxic phenotype) [39,40] and VEGF (angiogenic factor) [9], the molecular mechanism during the development to poor differentiation of HCC is not unknown. The supply of oxygen and nutrition from the tumor vessel is essential for the growth of tumor.…”