[1] Geochemical profiles were coupled with seismic information to examine subsurface hydrocarbon source, migration, and fate at a Gulf of Mexico carbonate-gas hydrate mound (Woolsey Mound). Three seafloor features were investigated in detail: (1) major faults resulting from a rising salt body, (2) an acoustic backscatter anomaly, and (3) a pockmark associated with a major fault. We analyzed sulfate, chloride, dissolved inorganic carbon, and hydrocarbon concentrations, and carbon isotopes in pore water extracted from 20 m piston cores to characterize gas source and calculate methane flux. Dissolved biogenic methane dominated the off-fault sites, while the contribution of thermogenic methane increased near a major fault where thermogenic gas hydrates were recovered. Within the pockmark, methane concentrations were low and isotopes indicated a biogenic source. Since pockmarks are typically formed from expulsive fluid flow, this suggests that either the pockmark is the legacy of a conduit that has become plugged or that the expulsed fluid is confined within the fault walls. At the acoustic anomaly,