Multiple Responses of Rhizobia to Flavonoids During Legume Root Infection

Cooper, J. E.

doi:10.1016/s0065-2296(04)41001-5

Cited by 154 publications

(103 citation statements)

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“…This finding is definitely intriguing when considering that the flavonoids generally have a fundamental role in protecting higher plants from biotic and abiotic stresses (Agati and Tattini 2010;Winkel-Shirley 2002). As demonstrated also in other rhizospheric microorganisms, plant-derived flavonoids play multiple roles, depending on their structure, such as to inhibit several phytopathogens, to stimulate mycorrhizal spore germination and hyphal branching, to mediate allelopathic interactions, and to chelate soil nutrients (Cooper 2004). Plant flavonoids have been shown to evoke a strong chemoattractant response toward plant roots in rhizobia (Caetano-Anolles et al 1988).…”

Section: Introductionmentioning

confidence: 95%

“…The rhizobia-legumes symbiosis development depends on a complex molecular signal exchange between the two partners that, in the early stages, involves the release from plant roots of flavonoids, which are essential for a successful infection and serve as key signals for the organogenesis of nitrogen-fixing nodules (Cooper 2004;Cooper 2007;Perret et al 2000;Shaw et al 2006). This finding is definitely intriguing when considering that the flavonoids generally have a fundamental role in protecting higher plants from biotic and abiotic stresses (Agati and Tattini 2010;Winkel-Shirley 2002).…”

Section: Introductionmentioning

confidence: 99%

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Effect of the plant flavonoid luteolin on Ensifer meliloti 3001 phenotypic responses

et al. 2015

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Background and aims The establishment of a successful symbiosis between the nitrogen-fixing bacterium Ensifer meliloti and compatible host legumes (Medicago spp.) depends on a complex molecular signal exchange. The early stage of signaling involves the release from plant roots of the flavonoid luteolin, which in turn induces the expression of rhizobia nodulation (nod) genes required for root infection and nodule development. To date, the bacterial response to the luteolin perception has been characterized in detail as far as gene expression is concerned. Nevertheless, despite this molecular information, a global view on E. meliloti phenotypes affected by the plant signal luteolin is still lacking.Therefore, an extensive phenotypic investigation of luteolin effect on the nitrogen-fixing E. meliloti 3001 has been performed. Methods A thousand different growth conditions, including sensitivity to osmolites, pH stresses, antibiotics and toxic compounds, were tested by the application of the high-throughput Phenotype MicroArray (PM) technology, as well as by several dedicated assays to evaluate growth stimulation, motility, biofilm formation, Nacyl homoserine lactones and Indole-3-acetic acid (IAA) production. Results Results revealed that the plant signal luteolin affected a wide spectrum of E. meliloti 3001 phenotypes. E. meliloti 3001 displayed an enhanced resistance phenotype in the presence of luteolin toward a broad set of chemicals including several antibiotics, toxic ions, respiration inhibitors, membrane damagers, DNA intercalants and other potential antimicrobial agents. Moreover, the presence of luteolin significantly reduced overall AHLs production, as well as the lag phase in relation to the starting cellular density, the motility and biofilm formation under nutrient-limited growth conditions. An effect on E. meliloti indole-3-acetic acid (IAA) production was also detected in vitro as a response to luteolin. Conclusions Overall, these findings suggest that the plant signal luteolin triggers a broad response in E. meliloti 3001, which was shown to be dependent on nutritional conditions sensed by the bacterium, pointing out a wide role in modifying rhizobial phenotypes, possibly in relation to plant root association and then symbiotic interaction.Plant Soil (2016) 399:159-178

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Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Effect of the plant flavonoid luteolin on Ensifer meliloti 3001 phenotypic responses

et al. 2015

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“…Gut bacteria can also modify dietary flavonoids (132) that have significant effects on animal physiology. Analogously, in roots, flavonoids produced by plants are signal molecules in bacteria (133) and are also transformed by bacteria in vitro, though this has not been shown in vivo. Plant phytoalexins are antimicrobials that are expelled from Rhizobium etli, Bradyrhizobium japonicum, and Agrobacterium by MDR efflux pumps that are inducible by root-exudated flavonoids (20,119,134).…”

Section: Similar Bacterium-host Interactions In Guts and Rootsmentioning

confidence: 99%

Gut and Root Microbiota Commonalities

Ramírez-Puebla¹,

Servín-Garcidueñas²,

Jiménez-Marín³

et al. 2013

Appl Environ Microbiol

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Animal guts and plant roots have absorption roles for nutrient uptake and converge in harboring large, complex, and dynamic groups of microbes that participate in degradation or modification of nutrients and other substances. Gut and root bacteria regulate host gene expression, provide metabolic capabilities, essential nutrients, and protection against pathogens, and seem to share evolutionary trends.

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“…The exudation of flavonoids by legumes is continuous, but the concentration of flavonoids increases when a compatible bacterium is found (Lira et al, 2015). Once in the rhizosphere, flavonoids act as chemoattractants toward the root, and passively diffuse across the bacterial membrane (Cooper, 2004 and no correlation has been demonstrated between the number of flavonoids a given NodD responds to and the host range of the bacterium. As an example, Rhizobium sp.…”

Section: Sd-cdsmentioning

confidence: 99%

“…The exudation of flavonoids by legumes is continuous, but the concentration of flavonoids increases when a compatible bacterium is found (Lira et al, 2015). Once in the rhizosphere, flavonoids act as chemoattractants toward the root, and passively diffuse across the bacterial membrane (Cooper, 2004, Wang et al, 2012. Flavonoids mediate the activation of NodD, a LysR-like transcriptional regulator, but no direct biochemical interaction with it has been shown.…”

Section: List Of Tablesmentioning

confidence: 99%

Genomics of Specificity in the Symbiotic Interaction between Rhizobium leguminosarum and Legumes

Rubio¹

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. Para el desarrollo de esta Tesis he contado con una beca UPM homologada financiada por el proyecto MICROGEN: Genómica Comparada Microbiana (Programa Consolider), que ha sido también el proyecto financiador del trabajo experimental.Quisiera reconocer la labor de aquellas personas que han contribuido al desarrollo y consecución de esta Tesis.Dr. Juan Imperial, por la dirección y supervisión de esta Tesis. Por ser un gran mentor y por enseñarme todo lo que sé.Dr. Manuel González Guerrero, por enseñarme los entresijos de la Ciencia y del trabajo en el laboratorio.Dra. Gisèle Laguerre, por su participación en el inicio de este proyecto y por facilitarnos el suelo P1 de Dijon.Dr. Paulo Arruda, por la formación que obtuve durante mi estancia en el laboratorio de Estudio de la Regulación y de la Expresión génica de la Universidad Estatal de Campinas.Dra. Belén Brito, por su gran ayuda en la primera fase de este proyecto y por recomendarme, sin ella nunca hubiera empezado esta Tesis.Rosabel Prieto, por su inestimable ayuda para la obtención de los Pool-Seqs y por enseñarme muchas de las técnicas que he necesitado durante la Tesis.Santiago de la Peña, por su valiosa ayuda en el análisis bioinformático de los genomas individuales.Dra. Julia Quintana, por enseñarme los conceptos de genética de poblaciones y por las largas discusiones científicas que tanto me han aportado.Dra. Viviana Escudero, por enseñarme a cuidar las plantas y valorar las cosas realmente importantes. Dra. Carmen Sánchez, por enseñarme las técnicas básicas de microbiología y genética molecular durante mi periodo como estudiante de máster.Dr. David Durán, por enseñarme los conceptos básicos de filogenia. XIII AGRADECIMIENTOSEscribir los agradecimientos es la parte más difícil de una tesis, no porque sea complicado, si no porque es la única parte que se lee todo el mundo y supone una gran presión añadida. Siempre me imaginaba escribir esta parte como algo extremadamente emotivo, pero después de escribir más de 200 páginas me he quedado sin palabras.La ciencia siempre fue lo que quise hacer, pero nunca pensé que pudiera hacerlo, pero… here I am! Ha sido un camino largo y duro, pero no lo cambiaría por nada, me he convertido en lo que quería y he conocido a los mejores. Desde que tengo 13 años sabía que quería estudiar esto, gracias a la inspiración de mi profesora de biología Marisa Alonso. Cuando llegué a la carrera, las cosas no fueron como esperaba, pero aún así descubrí mi pasión por la micro, la genética y las plantas… Y sobre todo conocí a grandes personas. Desde luego la mejor decisión que tomé fue hacer el máster, este fue el punto de inflexión, descubrí que era hacer ciencia… y eso me enganchó. Quiero agradecer a Pepe Palacios por acogerme en su laboratorio durante este periodo, ya que sin duda, mi paso por allí marcó mi vuelta. Por ello también quiero agradecer a todos los Rizobios y Levaduros, y en especial a Carmen Sánchez, por ser mi supervisora en los inicios y enseñarme muchas de las técnicas básicas de micro y genética molecular que he apre...

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Multiple Responses of Rhizobia to Flavonoids During Legume Root Infection

Cited by 154 publications

References 290 publications

Effect of the plant flavonoid luteolin on Ensifer meliloti 3001 phenotypic responses

Effect of the plant flavonoid luteolin on Ensifer meliloti 3001 phenotypic responses

Gut and Root Microbiota Commonalities

Genomics of Specificity in the Symbiotic Interaction between Rhizobium leguminosarum and Legumes

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