Multiple roles of CD90 in cancer

Kumar, Atul; Bhanja, Anshuman; Bhattacharyya, Jayanta; Jaganathan, Bithiah Grace

doi:10.1007/s13277-016-5112-0

Cited by 91 publications

(68 citation statements)

References 120 publications

(211 reference statements)

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“…In our experiments, FBS-free culture conditions did not only lead to reduced transfer of CD73 towards the cell membrane in immunocytochemistry images, but also led to a lesser abundance of CD73 mRNA. A lesser abundance in FBS-free media was similarly observed for CD90, which is also involved in the regulation of cell adhesion, migration, cell-cell and cell-matrix interactions [Kumar et al, 2016]. Collectively, these results suggest that FBS-free culture medium impairs the expression of stem cell adhesion molecules and, as shown for CD73, their presence on the cell surface.…”

Section: Discussionsupporting

confidence: 52%

Influence of Bovine Serum Lipids and Fetal Bovine Serum on the Expression of Cell Surface Markers in Cultured Bovine Preadipocytes

Sandhu¹,

Jurek²,

Trappe³

et al. 2017

Cells Tissues Organs

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To establish the influence of fetal bovine serum (FBS) and bovine serum lipids (BSL) on cell differentiation marker expression, bovine adipose-derived stem cells from subcutaneous tissue were incubated for 14 days in 4 types of differentiation media containing 10% FBS and 10 µL/mL BSL (TRT-1), no FBS and 10 µL/mL of BSL (TRT-2), 10% FBS and no BSL (TRT-3), or no supplements (TRT-4). Cells were subjected to Nile red staining, immunocytochemistry (CD73, CD90, CD105, DLK1, FabP4), and quantitative real-time PCR (CD73, CD90, CD105, FabP4). The number of cells presenting FabP4 and the percentage of mature adipocytes with large lipid droplets were increased in TRT-2, accompanied by a robust increase in FabP4 mRNA abundance and a decrease in DLK1-positive cells. In preadipocytes, CD73 was present around the nucleus and translocated towards cell membranes during differentiation. Although the percentage of CD73-positive cells was not different among treatments, its mRNA abundance, immunocytochemical staining intensity, and translocation towards cell membranes were decreased when the medium contained no FBS (TRT-2 and TRT-4). All cells showed a diffuse distribution of CD90 and CD105 and remained positive for these markers irrespective of the treatment. However, the CD90 and CD105 mRNA abundance was decreased in TRT-2 and TRT-4; i.e., in media containing no FBS. The presence of FBS increased the absolute number of cell nuclei as assessed by DAPI fluorescence. Our results suggest that bovine subcutaneous preadipocytes display typical stem cell markers. The differentiation into mature adipocytes is promoted by BSL, whereas FBS endorses cell proliferation.

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Section: Discussionsupporting

confidence: 52%

Influence of Bovine Serum Lipids and Fetal Bovine Serum on the Expression of Cell Surface Markers in Cultured Bovine Preadipocytes

Sandhu¹,

Jurek²,

Trappe³

et al. 2017

Cells Tissues Organs

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“…Although a clear significant interaction could not be demonstrated, it was interesting observing that a high infiltration of CD8a cells was only associated with a better prognosis within the CD90-dominant patient cluster. The role of CD90-positive fibroblasts in cancer progression and especially immune cell regulation is still mostly unknown and occasional reports are contradicting 57 especially with regard to their potential antigen presenting functions [46][47][48] . However, the given observations from this study support the hypothesis of functional differences between fibroblast subsets and experimental studies addressing this point would therefore be of high interest.…”

Section: Discussionmentioning

confidence: 99%

Fibroblasts in urothelial bladder cancer define stroma phenotypes that are associated with clinical outcome

Mezheyeuski

Segersten

Leiss

et al. 2020

Sci Rep

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Little attention was given to the interaction between tumor and stromal cells in urothelial bladder carcinoma (UBC). While recent studies point towards the existence of different fibroblast subsets, no comprehensive analyses linking different fibroblast markers to UBC patient survival have been performed so far. Through immunohistochemical analysis of five selected fibroblast markers, namely alpha smooth muscle actin (ASMA), CD90/Thy-1, fibroblast activation protein (FAP), platelet derived growth factor receptor-alpha and-beta (PDGFRa,-b), this study investigates their association with survival and histopathological characteristics in a cohort of 344 UBC patients, involving both, muscleinvasive and non-muscle-invasive cases. The data indicates that combinations of stromal markers are more suited to identify prognostic patient subgroups than single marker analysis. Refined stromamarker-based patient stratification was achieved through cluster analysis and identified a FAPdominant patient cluster as independent marker for shorter 5-year-survival (HR(95% CI)2.25(1.08-4.67), p = 0.030). Analyses of interactions between fibroblast and CD8a-status identified a potential minority of cases with CD90-defined stroma and high CD8a infiltration showing a good prognosis of more than 80% 5-year-survival. Presented analyses point towards the existence of different stroma-cell subgroups with distinct tumor-modulatory properties and motivate further studies aiming to better understand the molecular tumor-stroma crosstalk in UBC. The estimated incidence of urinary bladder cancer (UBC) worldwide was about 550.000 new cases and 200.000 deaths in 2018 1 , with highest incidences indicated in developed areas 2. Given that the prevalence of UBC is several times higher than the incidence, it represents a major burden for health care systems. Carcinomas originating from the urothelium, the inner surface of the bladder, represent the most common type of bladder cancer 3,4. Approximately 70% of all newly diagnosed cases are classified as non-muscle-invasive bladder cancer disease, while about 30% of patients have already muscle-invasive tumors with 10-15% of them being metastatic 5-8. Reported 5-year recurrence rates of non-muscle invasive cases range between 40-70% among the highest recurrence rates of solid tumors 3,9. Multidisciplinary approaches have been established in order to improve patient management and to decrease the mortality rate, but still treatment strategies for UBC remain controversial. A better understanding of the mechanisms underlying tumor progression is crucial and novel prognostic and predictive markers as well as therapeutic targets need to be identified 10,11. Recent research efforts on UBC aimed mostly to decipher molecular drivers of an invasive tumor phenotype through description of genetic varieties, and with the introduction of checkpoint inhibitory immunotherapy, awareness has also risen for tumor infiltrating leukocytes 12,13. However, little attention was given the interaction of tumor cells and non-leukoc...

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“…Reduced CD90 expression affects the intercellular interactions and adhesion of activated T-cells [26,46]. This interaction is supported by integrins.…”

Section: Cd90 Expression On Mscsmentioning

confidence: 96%

Alterations of Multipotent Mesenchymal Stromal Cells Induced by Interaction with Allogeneic Lymphocytes In Vitro

NM¹,

Nj²

2017

IJST

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Multiple roles of CD90 in cancer

Cited by 91 publications

References 120 publications

Influence of Bovine Serum Lipids and Fetal Bovine Serum on the Expression of Cell Surface Markers in Cultured Bovine Preadipocytes

Influence of Bovine Serum Lipids and Fetal Bovine Serum on the Expression of Cell Surface Markers in Cultured Bovine Preadipocytes

Fibroblasts in urothelial bladder cancer define stroma phenotypes that are associated with clinical outcome

Alterations of Multipotent Mesenchymal Stromal Cells Induced by Interaction with Allogeneic Lymphocytes In Vitro

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