Multiple roles of LncRNA-BMNCR on cell proliferation and apoptosis by targeting miR-145/CBFB axis in BMECs

Abstract

Bovine mastitis is one of the most serious and costly disease affecting dairy cattle production. The present study explored the inflammatory response and autoprotective mechanism of a novel specific high expression BMNCR (bovine mastitis related long non-coding RNA) in S. aureus induced mastitis by miR-145/CBFB axis in dairy cows from the perspective of molecular genetics. In bovine mammary epithelial cells, we preformed loss of function experiments to detect changes in cytokine, proliferation and apoptosis by qRT-PCR, western blot, flow cytometry and EdU staining. The results demonstrated that BMNCR significantly increased cell apoptosis, and inhibited cell proliferation. However, the secretion of IL-1α, IL-2, IL-6, IL-8 and IL-12 were enhanced after knock-down BMNCR. Bioinformatics analysis demonstrated that BMNCR could target 8 miRNAs, in-depth analyses indicated that BMNCR acts as a molecular sponge for bta-miR-145 and CBFB was one of 23 target gene of bta-miR-145 . The results of the present study demonstrated that the role of BMNCR in S. aureus induced mastitis can be mediated by sponge bta-miR-145 activating CBFB expression. BMNCR could be a potential target for mastitis diagnosis and therapy, which may enrich the theoretical research of therapeutic intervention, and further increase milk yield and improve milk quality.

Unveiling the Hidden Regulators: The Impact of lncRNAs on Zoonoses

“…The upregulation of SSR42 positively regulates the two-component system SaeRS, thereby promoting alpha-hemolysin expression, enhancing S. aureus pathogenicity, and potentially influencing cross-species transmission [ 89 , 90 ]. From the host’s perspective, the host-derived bovine mastitis-related long non-coding RNA ( BMNCR ) triggers an inflammatory response in bovine mammary glands through the miR-145/ CBFB axis, bolstering the autoprotective mechanism against S. aureus infection [ 91 ]. In addition, in bovine mammary epithelial cells, the antisense lncRNA LRRC75A-AS protects leucine rich repeat containing 75A ( LRRC75A ) mRNA from degradation by binding its coding sequence (CDS) region.…”