Multiple shoot induction and plant regeneration of the single-cell C4 species Bienertia sinuspersici

Northmore, Jennifer Anne; Zhou, Victoria L.; Chuong, Simon D. X.

doi:10.1007/s11240-011-0018-4

Cited by 9 publications

(3 citation statements)

References 45 publications

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“…Except for two reports on the regeneration using indirect organogenesis as well as direct shoot micropropagation [11] [12], no additional in vitro studies on the rapid vegetative propagation of B. sinuspersici have been documented. The purpose of this study is to examine the effects of Murashige and Skoog (MS) basal medium strengths and various auxins on the induction of adventitious roots in B. sinuspersici cuttings so that an efficient and rapid method for vegetative propagation of massive plant material can be established.…”

Section: Introductionmentioning

confidence: 99%

Effects of Media Composition and Auxins on Adventitious Rooting of Bienertia sinuspersici Cuttings

Northmore¹,

Leung²,

Chuong

2015

ABB

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An efficient in vitro method for rapid vegetative propagation of Bienertia sinuspersici, one of four terrestrial species of family Chenopodiaceae capable of performing C4 photosynthesis within a single cell, was developed. Cuttings of B. sinuspersici were used to examine the effects of Murashige and Skoog (MS) media strength and auxins on adventitious root formation. Half-strength MS medium was determined to be ideal for adventitious root formation in Bienertia cuttings. Although cuttings cultured in medium containing 5.0 mg/L α-naphthalene acetic acid (NAA) promoted the highest number of adventitious roots, cuttings cultured in medium supplemented with 1.0 mg/L indole-3-butyric acid (IBA) produced the longest adventitious roots and had the highest survival rate upon transplanting to soil. Histological analysis revealed variations in the root anatomy generated by the various auxins which may affect adventitious root formation and subsequent establishment of cuttings in soil. Overall, the established procedure provides a simple and cost-effective means for the rapid propagation of the single-cell C4 species B. sinuspersici.

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Section: Introductionmentioning

confidence: 99%

Effects of Media Composition and Auxins on Adventitious Rooting of Bienertia sinuspersici Cuttings

Northmore¹,

Leung²,

Chuong

2015

ABB

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“…This result is in agreement with Byrne and Caponetti (1992), who reported that 2,4-D and sucrose were necessary to produce calli in the Boston fern, Nephrolepis exaltata . Calli are very sensitive to in vitro culture conditions and easily turn brown (Ahloowalia, 1982; Northmore et al, 2012). It has been reported to be difficult for generating intact sporophytes from a callus in ferns (Kwa et al, 1997; Byrne and Caponetti, 1992).…”

Section: Discussionmentioning

confidence: 99%

Expression Analyses of Embryogenesis-Associated Genes during Somatic Embryogenesis of Adiantum capillus-veneris L. In vitro: New Insights into the Evolution of Reproductive Organs in Land Plants

Li¹,

Han

Fang

et al. 2017

Front. Plant Sci.

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An efficient in vitro regeneration system via somatic embryogenesis (SE) was developed for a fern species Adiantum capillus-veneris. Adventitious shoots, green globular bodies (GGBs) and calli were obtained with the maximal induction rate on the Murashige and Skoog (MS) medium of low concentrations of 6-benzyladenine (BA) (0–1.0 mg/L), 2.0 mg/L BA without 2,4-dichlorophenoxyacetic acid (2,4-D), 0.5 mg/L 2,4-D and 0.5–1.0 mg/L 6-BA, respectively. Cyto-morphological and histological changes in the shoot development via calli and GGBs were examined. For a better understanding of these developmental events, expression patterns of six genes, AcLBD16, AcAGL, AcBBM, AcWUS, AcRKD, and AcLEC1, were characterized during SE. AcBBM and AcLEC1 were ubiquitously expressed in direct SE (adventitious shoots and GGBs) the maximal expression of AcBBM in mature GGBs, and the high expression of AcLEC1 in GGB initiation and adventitious shoots. During the indirect SE, AcLBD16, AcLEC1, AcRKD, and AcWUS were highly expressed in mature calli. Additionally, phylogenetic analyses showed that AcWUS, AcBBM, AcLBD, AcAGL, AcRKD, and their homologs of other green plants formed monophyletic clades, respectively. Some of these gene families, however, diversified rapidly with the occurrence of embryophytes, suggesting that embryogenesis-associated genes could experience a rapid evolution with the colonization of plants to terrestrial environments. Expression and phylogenetic analyses of those embryogenesis-associated genes by the aid of in vitro regeneration system of A. capillus-veneris provide new insights into the evolution of reproductive organs in land plants.

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“…Several strategies are available for discovering the true nature of somaclonal variation, including phenotypic identification (Kong et al, 2011), cytological analysis (Konieczny et al, 2010), DNA analysis techniques (Torres Morán et al, 2010;Ai et al, 2011), and protein analysis (Northmore et al, 2012). In recent years, DNAbased molecular markers have served as a significant tool for evaluating heritable homogeneity and the trueto-type nature of in vitro propagated plants.…”

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Rejuvenation of mature lentisk by micrografting and evaluation of genetic stability

Onay¹,

Tilkat²,

Süzerer³

et al. 2016

Turk J Biol

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IntroductionMastic trees are commonly known as evergreen pistachio or lentisk. In Turkey lentisk shares its habitat with Pistacia terebinthus L., olives, carob, and others, but it prefers welldrained to dry sandy or stony alkaline soil conditions (Ak and Parlakcı, 2009). Mastic trees show a peculiar adaptability to several climatic conditions (Zohary, 1952) and have positive biological characteristics, such as drought tolerance (Correia and Catarino, 1994) and protection of soil against erosion (Mulas and Deidda, 1998). However, the area that is suitable for mastic tree cultivation is limited in the world. Today a major limitation facing the widespread expansion of commercial mastic tree plantations is the shortage of superior plants, primarily because of the difficulties experienced in propagating this species using the traditional method of rooting the cuttings. Currently mastic trees are proliferated by seed, with the rational development of hereditary instability and great discrepancy in the formation degree among genotypes because of obstacles such as parthenocarpy and ovary aborticide (Grundwag, 1976). Vegetative propagation by cutting is also difficult due to impoverished or adventitious roots. Conventional propagation methods to improve Pistacia species such as P. lentiscus L. are limited because species belonging to the genus Pistacia have a long stage of juvenility. In addition, plants originating from seeds often do not maintain the genetic purity of the parent plants due to segregation and recombination of genetic characteristics during sexual reproduction. Thus, to improve trade, a vegetative propagation technique must be developed for large-scale propagation. Possible ways to move forward are necessary in order to avoid problems in vegetative propagation and to produce clonal stocks. Micropropagation of lentisk genotypes has been achievedAbstract: An in vitro propagation method was established for both male and female genotypes of lentisk using actively growing shoot tips derived from forcefully lignified shoots. The effects of growth regulators on in vitro morphogenesis were investigated. Since rooting of the regenerated shoots for both genotypes was not achieved, an in vitro micrografting method was developed for the production of plantlets. Moreover, genetic stability of 3-, 6-, and 24-times subcultured clones of both genotypes was assessed and compared with the mother plants using fluorescent-based amplified fragment length polymorphism (AFLP) analysis. The set of main plants and the different subcultured clones were divided into two clusters. In the first cluster, the original male and female plants were grouped together with the 3-times subcultured female and the 6-times subcultured male and female groups, whereas the second cluster contained the 24-times subcultured clones and the 3-times subcultured male group. To the best of our knowledge, this is the first report of successfully inducing plantlets from mature lentisk genotypes and the first analysis of clonal fidelity of regenerated mature...

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Multiple shoot induction and plant regeneration of the single-cell C4 species Bienertia sinuspersici

Cited by 9 publications

References 45 publications

Effects of Media Composition and Auxins on Adventitious Rooting of Bienertia sinuspersici Cuttings

Effects of Media Composition and Auxins on Adventitious Rooting of Bienertia sinuspersici Cuttings

Expression Analyses of Embryogenesis-Associated Genes during Somatic Embryogenesis of Adiantum capillus-veneris L. In vitro: New Insights into the Evolution of Reproductive Organs in Land Plants

Rejuvenation of mature lentisk by micrografting and evaluation of genetic stability

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