Multiple shoot proliferation, bulblet induction and evaluation of genetic stability in Asiatic hybrid lily (Lilium sp.)

Yadav, Rakesh; Yadav, Neha; Pal, Minakshi; Goutam, Umesh

doi:10.1007/s40502-014-0060-4

Cited by 17 publications

(9 citation statements)

References 24 publications

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“…Lower frequency of polymorphic bands has also been reported in micropropagated plants of Aconitum violaceum [28] and in bulblets of Lilium sp. [14].…”

Section: Rapd and Issr Analysis Of Regenerated Microplantsmentioning

confidence: 99%

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An Improved Micropropagation and Assessment of Genetic Fidelity in Multipurpose Medicinal Tree, Acacia auriculiformis

Yadav

Kumar

2015

Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci.

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An efficient direct plant regeneration protocol has been developed for medicinal tree, Acacia auriculiformis that can be employed at a commercial scale. Bioactive properties of this tree attribute to spermicidal action and anti-HIV activity which has gained an increasing interest in world health sector. Among several media employed for present study, an utmost shoot regeneration frequency of 93 % with highest number 17.3 ± 0.61 of shoots per explant was obtained on MS medium supplemented with 2.0 mg l -1 KIN and 0.5 mg l -1 IAA. Inclusion of activated charcoal in media has effectively alleviated the browning problem of shoots. Maximum shoot length of 6.4 cm was attained on MS media supplemented with 0.25 mg l -1 GA 3 followed by rooting of individual microshoots on half strength MS media supplemented with 0.1 mg l -1 IAA. After successful hardening, rooted microshoots were maintained under greenhouse conditions. Genetic stability of micropropagated plants of A. auriculiformis have also been established via randomly amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers. A total of 83 (39 RAPD and 44 ISSR) clear, distinct and reproducible amplicons were produced. The banding patterns and similarity coefficients obtained by both RAPD and ISSR analysis have revealed no remarkable differences between tissue culture raised plants and the mother plant. This is the first report that evaluates the use of genetic markers to establish genetic fidelity of micropropagated A. auriculiformis. This method can be applied for the rapid clonal multiplication and true-to-type production of plant for attaining the ever increasing demand in pharmaceutical industries.

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“…Lower frequency of polymorphic bands has also been reported in micropropagated plants of Aconitum violaceum [28] and in bulblets of Lilium sp. [14].…”

Section: Rapd and Issr Analysis Of Regenerated Microplantsmentioning

confidence: 99%

“…Such absurdities are generally inherited and are therefore redundant in clonal propagation [14]. Genetic fidelity was also established via molecular marker based system for assurance of genetic stability of in vitro raised plants.…”

Section: Introductionmentioning

confidence: 99%

An Improved Micropropagation and Assessment of Genetic Fidelity in Multipurpose Medicinal Tree, Acacia auriculiformis

Yadav

Kumar

2015

Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci.

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“…Recent research on Lilium bulblet growth suggested that increasing auxin while lowering cytokinin contents might be useful to promote bulblet growth and development, whereas increasing cytokinin alone can be used to promote bulblet initiation [20,21]. Additionally, recent studies have revealed that GA promotes shoot growth and multiplication, whereas ABA, daminozide, and chlorocholine chloride significantly improved bulblet quality parameters, such as average size [22,23]. In addition, in Lilium, exogenously applied paclobutrazol, an inhibitor of GA biosynthesis, had an inhibitory effect that was commensurate with the concentration on the aerial and root parts of the bulblets, with low concentrations promoting and high concentrations inhibiting bulblet development [23].…”

Section: Introductionmentioning

confidence: 99%

Changes in carbohydrate metabolism and endogenous hormone regulation during bulblet initiation and development in Lycoris radiata

Yang

et al. 2020

BMC Plant Biol

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Background: Lycoris species have great ornamental and medicinal values; however, their low regeneration efficiency seriously restricts their commercial production. Understanding the mechanism of bulblet propagation in this genus, which has remained underexplored to date, could provide a theoretical basis for improving the reproductive efficiency. Therefore, we studied the bulblet initiation and developmental processes in Lycoris radiata. Results: We found that bulblets are formed on the junctions of the innermost layers of scales and the basal plate, and initially present as an axillary bud and gradually develop into a bulblet. We also determined the changes in carbohydrate and endogenous hormone contents during bulblet initiation and development, as well as the expression patterns of genes involved in carbohydrate metabolism and hormone biosynthesis and signaling through transcriptome analysis. Soluble sugars derived from starch degradation in the outer scales are transported to and promote bulblet initiation and development through starch synthesis in the inner scales. This process is mediated by several genes involved in carbohydrate metabolism, especially genes encoding ADP glucose pyrophosphorylase, a crucial starch synthesis enzyme. As for hormones, endogenous IAA, GA, and ABA content showed an increase and decrease during bulblet initiation and development, respectively, which were consistent with the expression patterns of genes involved in IAA, GA, and ABA synthesis and signal transduction. In addition, a decrease in ZR content may be down-and up-regulated by CK biosynthesis and degradation related genes, respectively, with increasing auxin content. Furthermore, expression levels of genes related to BR, JA, and SA biosynthesis were increased, while that of ethylene biosynthesis genes was decreased, which was also consistent with the expression patterns of their signal transduction genes. Conclusions: The present study provides insights into the effect of carbohydrate metabolism and endogenous hormone regulation on control of L. radiata bulblet initiation and development. Based on the results, we propose several suggestions to improve L. radiata propagation efficiency in production, which will provide directions for future research.

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“…Rhizomes also have a greater direct regeneration potential compared to other explants that are easier to obtain, such as leaves (Ma, Gang 2006). In Lilium, Narcissus and Tulipa, the modified leaf blades born in bulbs (i.e., an underground stem with fleshy, scale-like leaf blades surrounding the apical bud) are the most important source of material for micropropagation since even though they do not contain meristematic tissue, they are able to regenerate adventitious true-to-type bulbs (Jerzy, Krzymińska 2006;Yadav et al 2013). The level of contamination in corms and other underground organs can reach as high as 95 to 100% (Yasmin et al 2013).…”

Section: Theory Of Disinfection For the Establishment Of Plant Tissuementioning

confidence: 99%

Untitled

2016

EEB

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The choice of explant and its preparation are most likely one of the most important biotic factors that determine the success of a tissue culture protocol when the explant is derived from ex vitro organs. This review assesses the disinfection protocols available for saffron (Crocus sativus L.; Iridaceae) explants in an attempt to ascertain the most suitable set of parameters that could ensure successful tissue culture in subsequent treatments. From a methodological perspective, two explant types are most commonly adopted in saffron biotechnology, namely the use of fresh field-grown shoots, or dormant corms. The latter is more recommended to minimize contamination and to allow the use of more aggressive disinfection treatments.

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Multiple shoot proliferation, bulblet induction and evaluation of genetic stability in Asiatic hybrid lily (Lilium sp.)

Cited by 17 publications

References 24 publications

An Improved Micropropagation and Assessment of Genetic Fidelity in Multipurpose Medicinal Tree, Acacia auriculiformis

An Improved Micropropagation and Assessment of Genetic Fidelity in Multipurpose Medicinal Tree, Acacia auriculiformis

Changes in carbohydrate metabolism and endogenous hormone regulation during bulblet initiation and development in Lycoris radiata

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