Multiple Shoot Regeneration From the Cultures of Adhatoda Vasica Nees.

Tejavathi, D.H.; Manjula, B. L.; Anitha, P.

doi:10.17660/actahortic.2010.865.53

Cited by 2 publications

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“…Following this trend, 100% rooting with 3.5 roots/shoot of 4 cm length in 15 days was testified by Azad et al [32] in MS medium, devoid of any PGR. Nath and Buragohain [47] obtained as many as 9.33 roots per shoot of 0.6 cm length in PGR-free MS medium and a comparable result was reported by Tejavathi et al [50] as well. Apart from PGR-free MS medium, the most frequently used auxin was IBA.…”

Section: In Vitro Regenerationsupporting

confidence: 83%

“…Later on, Lone et al [37] added 0.5 mg/l NAA and 0.5 mg/l thidiazuron (TDZ) with 2 mg/l BA to improve the regeneration efficiency of BA; wherein, 100% of explants produced the maximum (23.3) shoots/explant in 28 days. However, in contrast, there are several reports on the sole use of BA for initiation of high frequency multiple shoots, wherein very high concentrations of 10 mg/l [8], [34] or 22.20 µm [50] BA were used. As an outcome, 93.33% explants produced ∼11 shoots/explant, each 5.2 cm in length.…”

Section: In Vitro Regenerationmentioning

confidence: 99%

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In vitro biotechnological advancements in Malabar nut (Adhatoda vasica Nees): Achievements, status and prospects

Gantait

Panigrahi²

2018

Journal of Genetic Engineering and Biotechnology

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Adhatoda vasica Nees, belonging to family Acanthaceae, is a well-known medicinal plant. It is endorsed for its pyrroloquinazoline alkaloids and its derivatives, such as vasicine and vasicinone. Germinating A. vasica seeds is a tedious task; on that account, vegetative propagation is the preferred method for its multiplication. For rapid and large-scale multiplication, germplasm conservation as well as secondary metabolites production, in vitro culture of A. vasica was preferred over conventional propagation by several researchers; however, some major applications of this tissue culture technique are still awaiting to undergo extensive research. The present review, for the first time, illustrates all the major achievements associated with in vitro regeneration of A. vasica , reported till date and highlights the future prospects.

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Section: In Vitro Regenerationsupporting

confidence: 83%

Section: In Vitro Regenerationmentioning

confidence: 99%

In vitro biotechnological advancements in Malabar nut (Adhatoda vasica Nees): Achievements, status and prospects

Gantait

Panigrahi²

2018

Journal of Genetic Engineering and Biotechnology

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“…In this study, the shoot tip gave suitable results for callusing of this species. Similar reports with this species were also made by other researchers [18,19]. The shoot tip culture has shown many advantages including better response and virus free results over other explants developed earlier [20].…”

Section: Selection Of Explants For Callus Initiationsupporting

confidence: 82%

Callus Formation and Regeneration Tendency of Justicia vasica Nees under Saline Conditions

Kader

Sinha

Ghosh

2021

Curr. Appl. Sci. Technol.

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Salinity is one of the major environmental threats to plants because a large number of plants are intolerant to NaCl stress. One such plant, Justicia vasica, which is the subject of our study, is said to have great medicinal properties. However, the natural occurrence and cultivation of this species has been constrained due to various factors including its poor seed germination rate under its normal conditions of habitat, and a low availability of seeds. This study was undertaken to examine the efficiency of callus formation and regenerative tendencies of Justicia vasica under saline conditions. The leaf, shoot tips and inter nodal cuttings were employed for this study using MS media formulated with several concentrations of different kinds of hormonal combinations. This study also tested the effects of different concentrations of NaCl on callus induction and growth. Each experiment was conducted thrice with 12 replicates. A superior level of callus formation was achieved by treating shoot tips with 2.0 mg/l α-naphthalene acetic acid (NAA) and 0.5 mg/l 6-benzyladenine purine (BAP) in combination. From this study, it was concluded that there was no inhibitory action from the medium supplemented with 0.05 mg/l NaCl. The optimal hormonal combination also exhibited highest rate of callus induction and growth with 0.05 mg/l NaCl added. There was no callus necrosis at this saline concentration after 60 days of inoculation. Furthermore, some somatic embryogenic callus displayed the organogenesis of leaf like structures after 75 days of inoculation. The development of salinity tolerance of plants by genetic application has been a vital area on modern agriculture as well as medicinal plant research because more land has been salinized through poor local irrigation practices. The present investigation assumed that the NaCl tolerance of this plant could be effectively obtained in vitro.

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Multiple Shoot Regeneration From the Cultures of Adhatoda Vasica Nees.

Cited by 2 publications

References 0 publications

In vitro biotechnological advancements in Malabar nut (Adhatoda vasica Nees): Achievements, status and prospects

In vitro biotechnological advancements in Malabar nut (Adhatoda vasica Nees): Achievements, status and prospects

Callus Formation and Regeneration Tendency of Justicia vasica Nees under Saline Conditions

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