Multiple SNPs Detection Based on Lateral Flow Assay for Phenylketonuria Diagnostic

Liu, Xiaonan; Zhang, Chao; Ke-wu, Liu; Wang, Han; Lu, Chaoxia; Li, Hang; Hua, Kai; Zhu, Jun‐Jie; Wang, Hui; Cui, Yali; Zhang, Xue

doi:10.1021/acs.analchem.7b05113

Cited by 54 publications

(38 citation statements)

References 50 publications

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“…These highlights make biosensors appropriate for point-of-care diagnostics because they can achieve quick and multi-analyte detection [67]. There are different types of biosensors including optical [68,69], electrochemical [70,71], piezoelectric [72,73], magnetic [74,75], micromechanical [76,77], and thermal [78,79,80,81] for medical diagnosis.…”

Section: Principle Of Biosensormentioning

confidence: 99%

An Alternative Medical Diagnosis Method: Biosensors for Virus Detection

et al. 2019

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Infectious diseases still pose an omnipresent threat to global and public health, especially in many countries and rural areas of cities. Underlying reasons of such serious maladies can be summarized as the paucity of appropriate analysis methods and subsequent treatment strategies due to the limited access of centralized and equipped health care facilities for diagnosis. Biosensors hold great impact to turn our current analytical methods into diagnostic strategies by restructuring their sensing module for the detection of biomolecules, especially nano-sized objects such as protein biomarkers and viruses. Unquestionably, current sensing platforms require continuous updates to address growing challenges in the diagnosis of viruses as viruses change quickly and spread largely from person-to-person, indicating the urgency of early diagnosis. Some of the challenges can be classified in biological barriers (specificity, low number of targets, and biological matrices) and technological limitations (detection limit, linear dynamic range, stability, and reliability), as well as economical aspects that limit their implementation into resource-scarce settings. In this review, the principle and types of biosensors and their applications in the diagnosis of distinct infectious diseases were comprehensively explained. The deployment of current biosensors into resource-scarce settings is further discussed for virus detection by elaborating the pros and cons of existing methods as a conclusion and future perspective.

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Section: Principle Of Biosensormentioning

confidence: 99%

An Alternative Medical Diagnosis Method: Biosensors for Virus Detection

et al. 2019

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“…4,7 Unfortunately, the clinical utility of cell-free DNA (cfDNA) in the maternal bloodstream was limited to isolating genes from the paternal Y chromosome, until the development of PCR-based techniques and-more recently-next-generation sequencing. 4,8,9 PCR-based sequencing methods are used reliably to diagnose single-gene disorders with cfDNA testing, but are not accurate for disorders with multiple causal factors. 8,9 Even with multi-gene accuracy, it would be too time-consuming to use a PCR-based method to examine multiple genes.…”

Section: Cell-free Dna Discovery and Importance Of Non-invasive Techniquesmentioning

confidence: 99%

“…4,8,9 PCR-based sequencing methods are used reliably to diagnose single-gene disorders with cfDNA testing, but are not accurate for disorders with multiple causal factors. 8,9 Even with multi-gene accuracy, it would be too time-consuming to use a PCR-based method to examine multiple genes. 8,9 Consequently, the demand for a non-invasive prenatal technique for multi-gene analysis resulted in the development of next-generation sequencing.…”

Section: Cell-free Dna Discovery and Importance Of Non-invasive Techniquesmentioning

confidence: 99%

“…8,9 Even with multi-gene accuracy, it would be too time-consuming to use a PCR-based method to examine multiple genes. 8,9 Consequently, the demand for a non-invasive prenatal technique for multi-gene analysis resulted in the development of next-generation sequencing. Next-generation sequencing provides greater sensitivity when…”

Section: Cell-free Dna Discovery and Importance Of Non-invasive Techniquesmentioning

confidence: 99%

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Cell-free DNA

Pickering

2020

UWOMJ

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Chromosomal disorders arise from errors in cell division and many are detected during prenatal development. Prenatal genomic screening techniques involve invasive methods such as chorionic villus sampling and amniocentesis. In this feature, current invasive techniques for genetic screening will be examined in relation to the development of non-invasive prenatal technology. As cell-free fetal DNA methods continue to develop and be integrated into clinical practice, there is an opportunity for improvement in the detection and reliability of the screening process. In clinic, there are disparities between clinicians and patients surrounding both understanding of the processes and the discussion on the technique limitations. Non-invasive methods are continually being improved for detecting genetic disorders through the use of cell-free fetal DNA, and with these advancements, these processes will become safe, cost-effective, and reliable for pregnant mothers when undergoing genetic screening and counselling.

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“…In our previous study, we established a lateral flow assay (LFA) assembled with GMNPs that relies on immune hybridization reactions for detecting SNPs, such as MTHFR C677T, which enables the typing of genomic DNA [21,22]. In this study, we demonstrate a Direct PCR LFA system, which amplifies nucleic acids without the need for DNA extraction and can be used for detecting I/D polymorphisms of large fragments.…”

Section: Introductionmentioning

confidence: 99%

A Novel PCR Method for Detecting ACE Gene Insertion/Deletion Polymorphisms and its Clinical Application

et al. 2021

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Background Angiotensin-converting enzyme (ACE) plays a major role in blood pressure regulation and cardiovascular homeostasis. The wide distribution and multifunctional properties of ACE suggest it’s involvement in various pathophysiological conditions. Results In this study, a novel visual detection method for ACE I/D polymorphisms was designed by integrating direct PCR without the need for DNA extraction using gold magnetic nanoparticles (GMNPs)-based lateral flow assay (LFA) biosensor. The entire detection procedure could enable the genotyping of clinical samples in about 80 min. The detection limit was 0.75 ng and results could be obtained in 5 min using the LFA device. Three hundred peripheral blood samples were analyzed using the direct PCR-LFA system and then verified by sequencing to determine accuracy and repeatability. A clinical preliminary study was then performed to analyze a total of 633 clinical samples. Conclusions After grouping based on age, we found a significant difference between the genotypes and the age of patients in the CHD group. The introduction of this method into clinical practice may be helpful for the diagnosis of diseases caused by large fragment gene insertions/deletions.

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Multiple SNPs Detection Based on Lateral Flow Assay for Phenylketonuria Diagnostic

Cited by 54 publications

References 50 publications

An Alternative Medical Diagnosis Method: Biosensors for Virus Detection

An Alternative Medical Diagnosis Method: Biosensors for Virus Detection

Cell-free DNA

A Novel PCR Method for Detecting ACE Gene Insertion/Deletion Polymorphisms and its Clinical Application

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