Multiple stimulation with spheroids comprising salivary gland and adipose-derived stem cells enhances regeneration of radiation-damaged salivary glands

Kim, Joo-Young; Eom, Min Rye; Jeong, Eun Ji; Choi, Ji Suk; Kwon, Seong Keun

doi:10.1016/j.jiec.2021.12.019

Cited by 3 publications

(6 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Kim et al . demonstrated lower expression of CD68 (monocyte lineage marker) and CD11b (leukocyte marker) in ADSC-seeded spheroid-grafted mice relative to irradiated controls [ 51 ]. Lindegren et al .…”

Section: Resultsmentioning

confidence: 99%

“…Kim et al . noted higher CD31 staining (IHC) and pro-angiogenic gene expression in ADSC-seeded spheroid-grafted mice compared to controls [ 51 ]. Huang et al .…”

Section: Resultsmentioning

confidence: 99%

“…Adem et al showed higher CD31 staining (IHC) in fat-grafted mice compared to irradiated controls [52]. Kim et al noted higher CD31 staining (IHC) and pro-angiogenic gene expression in ADSC-seeded spheroid-grafted mice compared to controls [51]. Huang et al also noted increased CD31 staining (IHC) and vascular density in the ASDCtreated rat group compared with the control group three weeks after fat grafting [47].…”

Section: Plos Onementioning

confidence: 99%

“…Immunomodulation. Kim et al demonstrated lower expression of CD68 (monocyte lineage marker) and CD11b (leukocyte marker) in ADSC-seeded spheroid-grafted mice relative to irradiated controls [51]. Lindegren et al noted that AFT decreased the macrophage density (CD86 IHC marker) in irradiated human breast tissue [39].…”

Section: Plos Onementioning

confidence: 99%

“…Kim et al, Sowa et al, and Adem et al studied the impact of 3-D biological structures on graft outcomes, with regards to ADSC-seeded spheroids, micronized cellular adipose matrices (MCAM), and decellularized adipose matrices (DAM) respectively [51][52][53]. In irradiated murine salivary glands, spheroid treatment appeared to restore both cellular architecture and functionality (mucin secretion).…”

Section: Plos Onementioning

confidence: 99%

See 4 more Smart Citations

Exploring the mechanisms behind autologous lipotransfer for radiation-induced fibrosis: A systematic review

Pattani,

Sanghera,

Langridge

et al. 2024

PLoS ONE

View full text Add to dashboard Cite

Aim Radiation-induced fibrosis is a recognised consequence of radiotherapy, especially after multiple and prolonged dosing regimens. There is no definitive treatment for late-stage radiation-induced fibrosis, although the use of autologous fat transfer has shown promise. However, the exact mechanisms by which this improves radiation-induced fibrosis remain poorly understood. We aim to explore existing literature on the effects of autologous fat transfer on both in-vitro and in-vivo radiation-induced fibrosis models, and to collate potential mechanisms of action. Method PubMed, Cochrane reviews and Scopus electronic databases from inception to May 2023 were searched. Our search strategy combined both free-text terms with Boolean operators, derived from synonyms of adipose tissue and radiation-induced fibrosis. Results The search strategy produced 2909 articles. Of these, 90 underwent full-text review for eligibility, yielding 31 for final analysis. Nine conducted in-vitro experiments utilising a co-culture model, whilst 25 conducted in-vivo experiments. Interventions under autologous fat transfer included adipose-derived stem cells, stromal vascular function, whole fat and microfat. Notable findings include downregulation of fibroblast proliferation, collagen deposition, epithelial cell apoptosis, and proinflammatory processes. Autologous fat transfer suppressed hypoxia and pro-inflammatory interferon-γ signalling pathways, and tissue treated with adipose-derived stem cells stained strongly for anti-inflammatory M2 macrophages. Although largely proangiogenic initially, studies show varying effects on vascularisation. There is early evidence that adipose-derived stem cell subgroups may have different functional properties. Conclusion Autologous fat transfer functions through pro-angiogenic, anti-fibrotic, immunomodulatory, and extracellular matrix remodelling properties. By characterising these mechanisms, relevant drug targets can be identified and used to further improve clinical outcomes in radiation-induced fibrosis. Further research should focus on adipose-derived stem cell sub-populations and augmentation techniques such as cell-assisted lipotransfer.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Kim et al . noted higher CD31 staining (IHC) and pro-angiogenic gene expression in ADSC-seeded spheroid-grafted mice compared to controls [ 51 ]. Huang et al .…”

Section: Resultsmentioning

confidence: 99%

Section: Plos Onementioning

confidence: 99%

Section: Plos Onementioning

confidence: 99%

Section: Plos Onementioning

confidence: 99%

See 3 more Smart Citations

Exploring the mechanisms behind autologous lipotransfer for radiation-induced fibrosis: A systematic review

Pattani,

Sanghera,

Langridge

et al. 2024

PLoS ONE

View full text Add to dashboard Cite

show abstract

Curcumin-laden ECM-mimicking microfibers assemble with mesenchymal stem cells to generate heterospheroids and enhance cell viability and function

Shrestha

Pathak²,

Regmi³

et al. 2022

Journal of Industrial and Engineering Chemistry

View full text Add to dashboard Cite

Research Progress on the Application of Injectable Hydrogel in Oral Tissue Regeneration

Wang,

Gao

2024

J Oral Pathology Medicine

View full text Add to dashboard Cite

BackgroundOral and maxillofacial tissue defects resulting from factors such as trauma or infection, can significantly impact both facial function and aesthetics. Additionally, the complex anatomical structure of the face often increases the difficulty of treatment. With the advantages of controlled release, targeted delivery, and enhanced mechanical properties, injectable hydrogels have been investigated for the treatment of oral and maxillofacial diseases. In the field of regeneration, injectable hydrogels have a structure similar to the extracellular matrix (ECM) and are biocompatible, which can be used as scaffolds for tissue regeneration.ObjectiveThis review aims to summarize the literature on the current status and limitations of injectable hydrogels in the field of oral tissue regeneration.MethodsWe searched Pubmed and Web of Science databases to find and summarize the articles on the application of injectable hydrogels in tissue regeneration.ConclusionsThis review focuses on the current status and limitations of injectable hydrogels in the field of tissue regeneration (periodontal tissue, dentin‐pulp complex, bone and cartilage, salivary gland regeneration, and mucosal repair). Although fully studied in animal models, there are still challenges in clinical transformation of injectable hydrogels in promoting tissue regeneration.

show abstract

Multiple stimulation with spheroids comprising salivary gland and adipose-derived stem cells enhances regeneration of radiation-damaged salivary glands

Cited by 3 publications

References 76 publications

Exploring the mechanisms behind autologous lipotransfer for radiation-induced fibrosis: A systematic review

Exploring the mechanisms behind autologous lipotransfer for radiation-induced fibrosis: A systematic review

Curcumin-laden ECM-mimicking microfibers assemble with mesenchymal stem cells to generate heterospheroids and enhance cell viability and function

Research Progress on the Application of Injectable Hydrogel in Oral Tissue Regeneration

Contact Info

Product

Resources

About