Multiple Strains of Candida albicans in the Oral Cavity of HIV Positive and HIV Negative Patients

Abstract

Over a period of several months a study of 14 HIV positive patients with oral infection due to Candida albicans revealed apparent changes in the strain carried by six patients (45 per cent) as determined by genomic analysis. In a second group of patients analysis of multiple isolates recovered from a single swab of the oral cavity revealed that one of ten HIV negative and four of ten HIV positive patients were carrying more than one strain. This may explain the apparent changes in type recorded in some individuals from the first group. Carriage of multiple strains of C. albicans may be more common in patients who are HIV positive. In addition, two major classes of pattern were seen designated X and Y; these were readily recognisable on RFLP profiles and especially after hybridisation with the 27A probe. The X type isolates behaved in all analyses as typical C albicans isolates in contrast to the type Y isolates which gave atypical results. The pattern Y strains appeared to be more common in HIV positive patients.

The Ins and Outs of DNA Fingerprinting the Infectious Fungi

“…Furthermore, DNA fingerprinting with Ca3 fulfills the general requirements set forth in a previous section of this review for effective fingerprinting methods (288). Ca3 and 27A have been used in a number of epidemiological studies of C. albicans and the highly related species C. dubliniensis (see, e.g., references 6,80,137,164,168,192,196,216,218,227,246,265,FIG. 4.…”

Candida dubliniensis: phylogeny and putative virulence factors

“…In addition to the reports of the increased detection of non-C. albicans species in cases of human infection, there have been several reports describing the emergence of what were originally described as 'atypical C. albicans' isolates (Mahrous et al, 1990;Schmid et al, 1992;Sullivan et al, 1993;McCullough et al, 1994McCullough et al, , 1995Boerlin et al, 1995;Le Guennec et al, 1995;Anthony et al, 1995). The isolates described by Sullivan et al (1993), McCullough et al (1994McCullough et al ( ,1995 and Boerlin et al (1995) were subjected to further detailed analysis (Sullivan et al, 1995(Sullivan et al, , 1997.…”

Candida albicans isolates from HIV-infected and AIDS patients exhibit enhanced adherence to epithelial cells

“…None was taking antibiotics at any time during the study. Some experiments were performed with BEC from six males with a mean age of 31 years (range 23-37) and six females with a mean age of 27 years (range [22][23][24][25][26][27][28][29][30]. However, the majority of experiments were done with BEC from a 22-yearold female.…”