Multiple toxic effects of tetraconazole in Allium cepa L. meristematic cells

Macar, Oksal

doi:10.1007/s11356-020-11584-4

Cited by 25 publications

(18 citation statements)

References 41 publications

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“…A statistically significant decrease compared to the control was observed in the length of the roots: by 18 % (at a concentration of 6.25 %), by 39 % (at a concentration of 12.5 %), by 62 % (at a concentration of 25.0 %), by 70 % (at a concentration of 50 %). The decrease in the length of the roots/aboveground part of the seedlings of test plants with increasing concentration of solutions studied for their toxicity, noted by other researchers (Seneviratne et al, 2017;Nedjimi, 2020;Pflugmacher et al, 2020Pflugmacher et al, , 2021Macar, 2021).…”

Section: Resultsmentioning

confidence: 59%

“…Test-indicators of L. sativum Various plants, including Allium cepa L. (Srivastava and Singh, 2020; Souza et al, 2020;Macar, 2021), Lactuca sativa L. (Lyu et al, 2018;Mtisi and Gwenzi, 2019;Gao et al, 2021), L. sativum (Szaniszló and Demény, 2018;Bosker et al, 2019;Pflugmacher et al, 2020Pflugmacher et al, , 2021Pignattelli et al, 2021a, b), are used in phytotoxicity studies of malathion, tetraconazole, nanoparticles, heavy metals, phenol, effluents, receiving water, coal ash, polyethylene particles, microplastics. In the studies of testing solutions toxicity are used following parameters of these plants: germination and radicle elongation (Mtisi and Gwenzi, 2019), germination, the root, the shoot, and the overall seedlings length, the roots and shoots' fresh and dry weight, the pigment Chl a and Chl b content, catalase activity (Pflugmacher et al, 2021).…”

Section: Resultsmentioning

confidence: 99%

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Toxicity of Aqueous Solutions of Cosmetics in Phytotest with Lepidium sativum L.

Tkachuk

2021

Agrobiodivers Improv Nutr health life Qual

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Garden cress (Lepidium sativum L.) is a test-plant for studying the toxicity of substrates, which is the basis of phytotesting. Dangerous pollutants are surfactants contained in household chemicals, including cosmetics. The purpose of the work is to investigate the toxicity of cosmetics the micellar water on a phytotest with garden cress and to analyze it for possible effects on human health and the environment. Investigated available in the retail network of Ukraine means for removing makeup and cleansing the skin -micellar water. Seed germination energy (3 rd day), seed germination, and biometric-morphometric parameters (length of roots and aboveground part of seedlings) (5 th day) were determined. The results were processed statistically. It was found that the germination rates of garden cress seeds and biometric indicators of seedlings significantly decrease (by 14-100 %) with the increasing concentration of the studied micellar water. The phytotoxic effect ranged from 49.6 % to 100 %. It is established that the value of the total toxicity index of solutions is from 0.55 (concentration 6.25 %) to 0 (concentration 100 %), indicating an increase in the toxicity of the solution with increasing concentration. Determined that garden cress is a sensitive plant to the studied cosmetic. The obtained data confirm the high efficiency of this test plant for use in biotesting. The phytotest with L. sativum established the lethal effect of this cosmetic product at a concentration of 100 %. Phytotoxicity decreases when the solution is diluted. Given the results of phytotesting and the composition of the cosmetic product, it can be assumed that at a concentration of 100 % it can pose a potential danger to human health. Given the increase in the market of perfumes and cosmetics, the emergence of counterfeit products, we can expect an increase in the impact of cosmetics on the quality of the environment.

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Section: Resultsmentioning

confidence: 59%

Section: Resultsmentioning

confidence: 99%

Toxicity of Aqueous Solutions of Cosmetics in Phytotest with Lepidium sativum L.

Tkachuk

2021

Agrobiodivers Improv Nutr health life Qual

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“…MN formation may be the result of breaks in microtubules and chromosomes or single-strand breaks in DNA (Konuk et al 2007;Qureshi et al 2016). Although no previous report was found about metaldehydeinduced MN formation, many researchers considered increasing MN frequencies as a sign on genotoxic effect of pesticides in plants (de Souza et al 2017;Datta et al 2018;Kalefetoğlu Macar 2020;Macar 2020). Parallel to the MN frequencies, frequencies of all CA types were also gradually increased by increasing doses of metaldehyde applications.…”

Section: Resultsmentioning

confidence: 99%

“…indicators of elevated level of reactive oxygen species (ROS) in A. cepa due to pesticides(Sivakumar et al 2010;Macar 2020). Similarly, excessive ROS production increases membrane lipid peroxidation in plants, which can be measured by MDA content(Srivastava and Sing, 2020).…”

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confidence: 99%

Metaldehyde-Mediated Genotoxicity in Allium Cepa (L.) Root Meristem Cells

Macar

2021

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The excessive use of metaldehyde (MA) in agriculture to combat molluscs endangers both the environment and non-target organisms. Allium cepa is a popular model organism for determining toxicity of commonly used chemicals such as pesticides. The aim of this study was to investigate the metaldehyde-mediated genotoxicity in A. cepa roots. To achieve this goal, five groups were formed and the first group considered as control was treated with tap water throughout the experiment. 20 ppm metaldehyde, 40 ppm metaldehyde, 100 ppm metaldehyde and 200 ppm metaldehyde solutions were applied to the other groups, respectively. Germination percentage, relative injury rate, root length and weight gain were analyzed to assess the effect of the pesticide on growth. Genotoxicity arisen from different doses of metaldehyde was investigated in terms of mitotic index (MI), micronucleus frequency (MN) and chromosomal aberrations (CAs). In addition, oxidative stress caused by metaldehyde was investigated by analyzing malondialdehyde (MDA) levels, as well as superoxide dismutase (SOD) and catalase (CAT) activities. The results of the growth parameters showed that metaldehyde had a growth-limiting effect in A. cepa, depending on the application dose. According to root elongation levels, EC50 (effective concentration) value for metaldehyde was 50 ppm in A.cepa. As the applied dose of metaldehyde increased, the incidence of MN and CAs gradually increased while MI decreased. Incrising doses of metaldehyde application also increased MDA levels, SOD and CAT activities. Data of the present study undoubtedly showed that metaldehyde had a suppressive effect in usual cell division, growth and chromosomal integrity while caused oxidative stress associated with genotoxicity.

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“…In this regard, Allium cepa L. is an ideal model organism due to its rapid growth rate of its roots. Therefore, Allium bioassay offers a versatile, convenient and practical test system that is often utilized to assess the possible genotoxicity and cytotoxicity of environmental contaminants (Kalefetoğlu Macar et al 2020;Macar 2020;Öztürk et al 2020). An important reason for choosing the Allium model is that this organism has an excellent correlation with mammalian test systems (Srivastava and Singh 2020).…”

Section: Introductionmentioning

confidence: 99%

A Comparison of Cytotoxic and Genotoxic Damages Induced by 254 Nm and 365 Nm Radiation: Allium Cepa L. Model

Çavuşoğlu

Macar

et al. 2021

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Living organisms are increasingly exposed to ultraviolet (UV) rays of solar radiation, both due to the thinning of the ozone layer and the widespread uses in sterilization processes. The present study was conducted with the purpose of evaluating the damages of UV-A and UV-C radiations in Allium cepa L. roots. Three groups were formed from Allium bulbs, one of which was the control group. One of the other groups was exposed to 254 nm (UV-C) and the other to 365 nm (UV-A) UV. Growth retardation effect of UV was investigated with respect to germination percentage, total weight gain and root elongation, while genotoxicity arisen from UV exposure analyzed using mitotic index (MI) and chromosomal aberrations (CAs) including micronucleus (MN) frequency. Oxidative stress due to UV application was investigated based on the accumulation of malondialdehyde (MDA) and the total activities of superoxide dismutase (SOD) and catalase (CAT) enzymes. Also, meristematic integrity of the UV treated roots was controlled. UV treatments caused significant changes in all parameters compared to the control, but all effects were much more prominent in 254 nm UV-exposed group. This study clearly revealed that UV exposure triggered growth inhibition, genotoxicity, oxidative stress and meristematic cell damages in A. cepa roots depending on the wavelength.

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Multiple toxic effects of tetraconazole in Allium cepa L. meristematic cells

Cited by 25 publications

References 41 publications

Toxicity of Aqueous Solutions of Cosmetics in Phytotest with Lepidium sativum L.

Toxicity of Aqueous Solutions of Cosmetics in Phytotest with Lepidium sativum L.

Metaldehyde-Mediated Genotoxicity in Allium Cepa (L.) Root Meristem Cells

A Comparison of Cytotoxic and Genotoxic Damages Induced by 254 Nm and 365 Nm Radiation: Allium Cepa L. Model

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