Multiple typing of strains of Salmonella enterica subsp. Bongori ser. 48:z35:- isolated in southern Italy

Nastasi, A; Mammina, Caterina; Villafrate, M. R.; Massenti, Maria Fatima; Scarlata, G; Diquattro, M

doi:10.1016/0769-2609(88)90158-5

Cited by 13 publications

(10 citation statements)

References 11 publications

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“…In particular, Y. enterocolitica and S. bongori, which caused restricted outbreaks of serious enteric episodes in children in several areas of Sicily, were detected in the present investigation (Nastasi et al, 1988;Pignato et al, 1998). Research has shown that the prevalence of S. bongori 48:z35:-in the study area is low but sufficiently continuous to be indicative of the epidemiological characteristics of Sicily (Giammanco et al, 2002).…”

Section: Discussionsupporting

confidence: 47%

Pathogenic microorganisms carried by migratory birds passing through the territory of the island of Ustica, Sicily (Italy)

et al. 2011

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Several studies have shown that migratory birds play an important role in the ecology, circulation and dissemination of pathogenic organisms. In October 2006, a health status evaluation was performed on a large population of migratory birds passing through the territory of Ustica (Italy), an island located on the migration route of many species of birds to Africa, and various laboratory tests were conducted. In total, 218 faecal swabs and the internal organs of 21 subjects found dead in nets were collected for bacteriological and virological examination, including avian influenza and Newcastle disease. In addition, 19 pooled fresh faecal samples were collected for mycological examination. The bacteriological analysis produced 183 strains belonging to 28 different species of the Enterobacteriaceae family. In particular, Salmonella bongori, Yersinia enterocolitica and Klebsiella pneumonia strains were isolated. Almost all of the isolates were susceptible to sulphamethoxazole/trimethoprime (99.4%), cefotaxime (98.9%), nalidixic acid (96.7%), chloramphenicol (95.6%), and tetracycline (93.4%). Alternatively, many strains were resistant to ampicillin (42.6%), amoxicillin-clavulanic acid (42.6%), and streptomycin (43.7%). According to reverse transcriptase-polymerase chain reaction analysis, all of the samples were negative for the M gene of avian influenza virus. Moreover, isolation tests conducted on specific pathogen free eggs were negative for avian influenza and Newcastle disease. Several hyphomycetes and yeasts belonging to different genera were present in the specimens, and Cryptococcus neoformans was observed in a pooled faecal sample. Antibiotic resistance in wildlife can be monitored to evaluate the impact of anthropic pressure. Furthermore, migratory birds are potential reservoirs of pathogenic agents; thus, they can be regarded as sentinel species and used as environmental health indicators.

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Section: Discussionsupporting

confidence: 47%

Pathogenic microorganisms carried by migratory birds passing through the territory of the island of Ustica, Sicily (Italy)

et al. 2011

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“…The antigen O48 of Salmonella occurs in several serovars and, historically, was classified as serogroup Y [9]. Serotype O48 belongs to clinically important bacteria causing intestinal disfunctions and diarrhoea in infants and children [30].…”

Section: Discussionmentioning

confidence: 99%

Structure of the sialic acid‐containing O‐specific polysaccharide from Salmonella enterica serovar Toucra O48 lipopolysaccharide

Gamian

Jones

Lipiński

et al. 2000

European Journal of Biochemistry

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Lipopolysaccharide was extracted from cells of Salmonella enterica serovar Toucra O48 and, after mild acid hydrolysis (1% AcOH, 1 h, 100 8C or 0.1 m NaOH-AcOH, pH 4.5, 5 h, 100 8C), the O-specific polysaccharide was isolated and characterized. The core and an oligosaccharide containing a fragment of the repeating unit linked to the core region were also obtained, depending on hydrolysis conditions. On the basis of sugar and methylation analyses and NMR spectroscopy of the hydrolysis products, the biological repeating unit of the O-specific polysaccharide was shown to be the following trisaccharide:The polysaccharide O-chain was substituted with a single molar equivalent of O-acetyl group, distributed between the Neu5Ac O-9 and O-7 positions, in an approximate ratio of 7 : 3.

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“…Several authors [2,3,17,18,[28][29] have described serovars containing plasmids of various molecular size which did not contain the genes associated with virulence. The search for the plasmid content of strains was often used in the study of epidemiological markers [8,29,30 (4), hessarek, typhimurium (6) the advantage of being quick, reliable and easy to use, in comparison with conventional techniques.…”

Section: Discussionmentioning

confidence: 99%

“…The search for the plasmid content of strains was often used in the study of epidemiological markers [8,29,30 (4), hessarek, typhimurium (6) the advantage of being quick, reliable and easy to use, in comparison with conventional techniques. The standard methods used to investigate the relationship between the presence of a plasmid and increased virulence call for preliminary extraction of the plasmids followed by the study, in mice, of the effect of strains with or without their plasmids, or the use of DNA/DNA hybridization techniques [7,9,10,11,13,16,21,26].…”

Section: Discussionmentioning

confidence: 99%

Polymerase chain reaction for salmonella virulence–associated plasmid genes detection: a new tool in salmonella epidemiology

Rexach¹,

Dilasser²,

Fach³

1994

Epidemiol. Infect.

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SUMMARYThe important role of plasmid genes in assessing virulence for BALB/c mice in salmonella, and the difficulty of using standard techniques to detect them, led us to develop a detection method by gene amplification.One hundred and forty-three strains (71 serovars) of salmonella and 35 strains of other species were tested using specific oligonucleotide primers. The amplification products were identified by a specific oligonucleotide probe. Forty-nine salmonella strains from ten serovars (S. abortus ovis, S. choleraesuis, S. dublin, S. enteritidis, S. gallinarum/pullorum, S. hessarek, S. typhimurium, S. IIIa 48: Z4, Z23, S. IV43:z4, Z23: -, S. V 28: a: -) produced a positive and specific response.Because of various origins of the strains possessing the gene sought and the diversity of the responses, both from one serovar to another and in the same serovar, this search has its place among the epidemiological markers in general use. This method appears well suited to the research and detection of plasmid genes associated with mouse virulence in salmonella.

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Multiple typing of strains of Salmonella enterica subsp. Bongori ser. 48:z35:- isolated in southern Italy

Cited by 13 publications

References 11 publications

Pathogenic microorganisms carried by migratory birds passing through the territory of the island of Ustica, Sicily (Italy)

Pathogenic microorganisms carried by migratory birds passing through the territory of the island of Ustica, Sicily (Italy)

Structure of the sialic acid‐containing O‐specific polysaccharide from Salmonella enterica serovar Toucra O48 lipopolysaccharide

Polymerase chain reaction for salmonella virulence–associated plasmid genes detection: a new tool in salmonella epidemiology

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