Multiplex 16S rRNA‐derived geno‐biochip for detection of 16 bacterial pathogens from contaminated foods

Abstract

Foodborne diseases caused by various pathogenic bacteria occur worldwide. To prevent foodborne diseases and minimize their impacts, it is important to inspect contaminated foods and specifically detect many types of pathogenic bacteria. Several DNA oligonucleotide biochips based on 16S rRNA have been investigated to detect bacteria; however, a mode of detection that can be used to detect diverse pathogenic strains and to examine the safety of food matrixes is still needed. In the present work, a 16S rRNA gene‐derived geno‐biochip detection system was developed after screening DNA oligonucleotide specific capture probes, and it was validated for multiple detection of 16 pathogenic strains that frequently occur with a signature pattern. rRNAs were also used as detection targets directly obtained from cell lysates without any purification and amplification steps in the bacterial cells separated from 8 food matrixes by simple pretreatments. Thus, the developed 16S rRNA‐derived geno‐biochip can be successfully used for the rapid and multiple detection of the 16 pathogenic bacteria frequently isolated from contaminated foods that are important for food safety.

Expanded single-color barcoding in microspheres with fluorescence anisotropy for multiplexed biochemical detection

“…The probe and detection oligo-DNA sequences (Table S1, ESI†) were designed based on species specificity and screened from the 16S rRNA database without pseudo-positive results in real sample analyses. 36…”

Simultaneous Detection of Five Foodborne Pathogens Using a Mini Automatic Nucleic Acid Extractor Combined with Recombinase Polymerase Amplification and Lateral Flow Immunoassay

“…Therefore, early identification, monitoring and warning of foodborne pathogens are essential to prevent the occurrence of foodborne diseases. These initiatives could help to identify changing trends in specific diseases and reduce the risk factors and disease burden [ 14 ].…”

Editorial: Asian Congress of Biotechnology 2015

“…Their research provides a stable animal model of stroke with low variability, and this model can facilitate development of novel hMSC‐based therapeutics for stroke. Prof. Hyung Joon Cha and co‐workers describe the development of a new DNA microarray platform for the multiple detection of different bacterial pathogens from contaminated foods. By employing 16S rRNA gene‐derived different capture probes, the authors demonstrate successful detection of 16 pathogenic strains, which are recognized as important targets in food industries and public hygiene assessment.…”