Multiplex Analysis on a Single Porous Hydrogel Bead with Encoded SERS Nanotags

Abstract

Bead-based assays have drawn more and more attention in biomedical fields. Herein, we proposed a novel approach to achieve multiplex analysis on a single porous hydrogel bead (PHB) with Raman dyes (RDs) encoded core-shell surface-enhanced Raman scattering (SERS) nanotags. Because of the amplified signal of RDs by core-shell metal structure of the nanotag and the high surface area to volume ratio (SVR) of the PHB, the sensitivity and linear dynamic range (LDR) of the as-proposed multiplex analysis method are significantly improved. We anticipate this approach to be widely used in the multiplex assays and biosensors.

The Horizon of the Emulsion Particulate Strategy: Engineering Hollow Particles for Biomedical Applications

“…2019, 31, 1801159 Figure 7. [73] By simply altering the diameters of the assembled nanoparticles, multicolor beads, with different characteristic reflection peaks were prepared for multiplex encoding. a,b) Schematic illustration of the self-barcode particles, with opal structure (a) and inverse-opal structures (b).…”

Selectively enhanced Raman/fluorescence spectra in photonic–plasmonic hybrid structures

“…Recently, as an emerging eld, the photonic-plasmonic hybrid structure has drawn extensive attention in the SES study. [26][27][28][29][30][31][32][33][34][35] The hybrid structure works as an optoplasmonic system to simultaneously benet from high Q-factors of a photonic structure, as well as small modal volumes and extreme near-eld enhancements of a plasmonic structure, 36 which will be helpful to improve the enhancement ability, practicality, and versatility of an SES substrate. For example, Ahn et al demonstrated enhanced uorescence of dye molecules within a designed photonic-plasmonic structure constructed by putting a microsphere exactly into a plasmonic cavity.…”

“…32 Liu et al proposed a photonic-plasmonic hybrid structure constructed with an inverse three-dimensional photonic crystal and Au nanoparticles, and demonstrated its SERS application for the multi-objective detection of biomolecules. 33 Despite these achievements, there are still some challenges in the SES study based on photonic-plasmonic hybrid structures, which need urgent action. For example, the mechanism of SES in the optoplasmonic system is yet to be elucidated.…”

Charge Transfer Tuned by the Surrounding Dielectrics in TiO2-Ag Composite Arrays

“…One main approach to improving the efficiency of such materials is the addition of nanoparticles to the titanium surface, which can trap electrons and lessen the recombination of electron-hole pairs [16,17]. TiO 2 modified with Ag has been proven to restrain the recombination of photo-excited electrons and holes, which can improve the photocatalytic performance [18]. In our recent work, nanocap arrays of TiO 2 /Ag and co-sputtering TiO 2 -Ag were fabricated on two-dimensional colloidal arrays [19].…”