Multiplex CRISPR/Cas9-mediated genome editing to address drought tolerance in wheat

Abdallah, Naglaa A.; Elsharawy, Hany; Abulela, Hamiss A.; Thilmony, Roger; Abdelhadi, Abdelhadi A.; Elarabi, Nagwa I.

doi:10.1080/21645698.2022.2120313

Cited by 34 publications

(16 citation statements)

References 57 publications

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“…The studies on TaSal1 knockout wheat mutants obtained using CRISPR-Cas9 confirmed PAP accumulation, resulting in enhanced stress signaling and induced stomatal closure. Consequently, mutant plants had bent stem and rolled-leaf phenotype with better regulation of stomatal closure and seed germination ( Abdallah et al., 2022 ).…”

Section: Cellular and Molecular Responses To Cope With The Main Abiot...mentioning

confidence: 99%

“…As previously mentioned in this review, the phosphatase SAL1 negatively regulates plants’ response to drought ( Chan et al., 2016 ). Using the CRISPR-Cas9 system, scientists have already generated Tasal1 knockout mutant wheat with fewer and smaller stomata, that germinate and grow better under drought conditions ( Abdallah et al., 2022 ). Likewise, modifying the expression of transcription factors related to abiotic stress is another alternative for producing tolerant plants.…”

Section: Genome Editing: a Tool For Developing Stress Resistant Forag...mentioning

confidence: 99%

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Improving abiotic stress tolerance of forage grasses – prospects of using genome editing

Sustek-Sánchez

Rognli²,

Rostoks³

et al. 2023

Front. Plant Sci.

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Due to an increase in the consumption of food, feed, and fuel and to meet global food security needs for the rapidly growing human population, there is a necessity to obtain high-yielding crops that can adapt to future climate changes. Currently, the main feed source used for ruminant livestock production is forage grasses. In temperate climate zones, perennial grasses grown for feed are widely distributed and tend to suffer under unfavorable environmental conditions. Genome editing has been shown to be an effective tool for the development of abiotic stress-resistant plants. The highly versatile CRISPR-Cas system enables increasingly complex modifications in genomes while maintaining precision and low off-target frequency mutations. In this review, we provide an overview of forage grass species that have been subjected to genome editing. We offer a perspective view on the generation of plants resilient to abiotic stresses. Due to the broad factors contributing to these stresses the review focuses on drought, salt, heat, and cold stresses. The application of new genomic techniques (e.g., CRISPR-Cas) allows addressing several challenges caused by climate change and abiotic stresses for developing forage grass cultivars with improved adaptation to the future climatic conditions. Genome editing will contribute towards developing safe and sustainable food systems.

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Section: Cellular and Molecular Responses To Cope With The Main Abiot...mentioning

confidence: 99%

Section: Genome Editing: a Tool For Developing Stress Resistant Forag...mentioning

confidence: 99%

Improving abiotic stress tolerance of forage grasses – prospects of using genome editing

Sustek-Sánchez

Rognli²,

Rostoks³

et al. 2023

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…For instance, the gibberellin insensitive dwarf1 ( GID1 ) gene [ 82 ] and the LBD40 gene were edited [ 83 ]. In wheat, the SAL1 gene negative regulator of drought tolerance was edited through multiplex CRISPR/Cas9 assay that improves drought tolerance at the seedling stage [ 120 ]. Cotton’s ability to withstand drought can be improved by CRISPR/Cas genome editing of the HB12 gene [ 121 ].…”

Section: Crispr For Improving Drought Tolerance In Crop Plantsmentioning

confidence: 99%

Engineering Abiotic Stress Tolerance in Crop Plants through CRISPR Genome Editing

Rahman

Zulfiqar

Raza

et al. 2022

Cells

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Environmental abiotic stresses challenge food security by depressing crop yields often exceeding 50% of their annual production. Different methods, including conventional as well as genomic-assisted breeding, mutagenesis, and genetic engineering have been utilized to enhance stress resilience in several crop species. Plant breeding has been partly successful in developing crop varieties against abiotic stresses owning to the complex genetics of the traits as well as the narrow genetic base in the germplasm. Irrespective of the fact that genetic engineering can transfer gene(s) from any organism(s), transgenic crops have become controversial mainly due to the potential risk of transgene-outcrossing. Consequently, the cultivation of transgenic crops is banned in certain countries, particularly in European countries. In this scenario, the discovery of the CRISPR tool provides a platform for producing transgene-free genetically edited plants—similar to the mutagenized crops that are not extensively regulated such as genetically modified organisms (GMOs). Thus, the genome-edited plants without a transgene would likely go into the field without any restriction. Here, we focused on the deployment of CRISPR for the successful development of abiotic stress-tolerant crop plants for sustaining crop productivity under changing environments.

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Section: Of 33mentioning

confidence: 99%

“…As previously mentioned in this review, the phosphatase SAL1 negatively regulates plants' response to drought [181]. Using the CRISPR-Cas9 system, scientists have already generated Tasal1 knockout mutant wheat with fewer and smaller stomata, that germinate and grow better under drought conditions [116]. Likewise, modifying the expression of transcription factors related to abiotic stress is another alternative for producing tolerant plants.…”

Section: Gene Editing: a Tool For Developing Stress Resistant Forage ...mentioning

confidence: 99%

Improving Abiotic Stress Tolerance of Forage Grasses – Prospects of Using Genome Editing

Sustek-Sánchez¹,

Rognli²,

Rostoks³

et al. 2022

Preprint

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Due to an increase in the consumption of food, feed, and fuel and to meet global food security needs for the rapidly growing human population, there is a necessity to obtain high-yielding crops that can adapt to future climate changes. Currently, the main feed source used for ruminant livestock production is forage grasses. In temperate climate zones, perennial grasses grown for feed are widely distributed and tend to suffer under unfavorable environmental conditions. Gene editing has been shown to be an effective tool for the development of abiotic stress-resistant plants. The highly versatile CRISPR-Cas system enables increasingly complex modifications in genomes while maintaining precision and low off-target frequency mutations. In this review, we provide an overview of forage grass species that have been subjected to gene editing. We offer a perspective view on the generation of plants resilient to abiotic stresses. Due to the broad factors contributing to these stresses the review focuses on drought, salt, heat, and cold stresses. The application of new genomic techniques (e.g., CRISPR-Cas) allows addressing several challenges caused by climate change and abiotic stresses for developing forage grass cultivars with improved adaptation to the future climatic conditions. Gene editing will contribute towards developing safe and sustainable food systems.

show abstract

Multiplex CRISPR/Cas9-mediated genome editing to address drought tolerance in wheat

Cited by 34 publications

References 57 publications

Improving abiotic stress tolerance of forage grasses – prospects of using genome editing

Improving abiotic stress tolerance of forage grasses – prospects of using genome editing

Engineering Abiotic Stress Tolerance in Crop Plants through CRISPR Genome Editing

Improving Abiotic Stress Tolerance of Forage Grasses – Prospects of Using Genome Editing

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