Multiplex Detection of Aspergillus fumigatus Mycoviruses

Özkan-Kotiloğlu, Selin; Coutts, Robert H.A.

doi:10.3390/v10050247

Cited by 7 publications

(4 citation statements)

References 27 publications

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“…A. fumigatus is regularly reported to be infected by viruses (592,593). The presence of these viruses may have a role in fungal virulence, since the sequenced strain Af293, which is infected with a mycovirus, is less pathogenic than other wild-type strains (176).…”

Section: Some Unexplored Biological Features Of a Fumigatusmentioning

confidence: 99%

Aspergillus fumigatus and Aspergillosis in 2019

2019

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SUMMARY Aspergillus fumigatus is a saprotrophic fungus; its primary habitat is the soil. In its ecological niche, the fungus has learned how to adapt and proliferate in hostile environments. This capacity has helped the fungus to resist and survive against human host defenses and, further, to be responsible for one of the most devastating lung infections in terms of morbidity and mortality. In this review, we will provide (i) a description of the biological cycle of A. fumigatus; (ii) a historical perspective of the spectrum of aspergillus disease and the current epidemiological status of these infections; (iii) an analysis of the modes of immune response against Aspergillus in immunocompetent and immunocompromised patients; (iv) an understanding of the pathways responsible for fungal virulence and their host molecular targets, with a specific focus on the cell wall; (v) the current status of the diagnosis of different clinical syndromes; and (vi) an overview of the available antifungal armamentarium and the therapeutic strategies in the clinical context. In addition, the emergence of new concepts, such as nutritional immunity and the integration and rewiring of multiple fungal metabolic activities occurring during lung invasion, has helped us to redefine the opportunistic pathogenesis of A. fumigatus.

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Section: Some Unexplored Biological Features Of a Fumigatusmentioning

confidence: 99%

Aspergillus fumigatus and Aspergillosis in 2019

2019

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“…Moreover, sputa collected from CF patients contained high number of phages which can be stimulated by biofilm or hypoxia conditions during bacterial growth [19]. Many A. fumigatus strains are strains host viruses intracellularly suggesting a putative role in horizontal gene transfer [20].…”

Section: The Lung Microbiota In Different Clinical Situationsmentioning

confidence: 99%

Interactions between Aspergillus fumigatus and Pulmonary Bacteria: Current State of the Field, New Data, and Future Perspective

Briard

Mislin

Latgé

et al. 2019

JoF

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Aspergillus fumigatus and Pseudomonas aeruginosa are central fungal and bacterial members of the pulmonary microbiota. The interactions between A. fumigatus and P. aeruginosa have only just begun to be explored. A balance between inhibitory and stimulatory effects on fungal growth was observed in mixed A. fumigatus–P. aeruginosa cultures. Negative interactions have been seen for homoserine-lactones, pyoverdine and pyochelin resulting from iron starvation and intracellular inhibitory reactive oxidant production. In contrast, several types of positive interactions were recognized. Dirhamnolipids resulted in the production of a thick fungal cell wall, allowing the fungus to resist stress. Phenazines and pyochelin favor iron uptake for the fungus. A. fumigatus is able to use bacterial volatiles to promote its growth. The immune response is also differentially regulated by co-infections.

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“…However, these methods could not distinguish the four P. ostreatus mycoviruses in one reaction. Multiplex RT-PCR has become a powerful tool for the simultaneous detection of several different viruses at a time [ 39 , 40 ]. In the present study, we developed a multiplex RT-PCR assay for the simultaneous detection of the four known P. ostreatus viruses.…”

Section: Introductionmentioning

confidence: 99%

Multiplex Detection of Pleurotus ostreatus Mycoviruses

Zhang

Wang

et al. 2022

CIMB

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Mycoviruses are viruses that specifically infect and replicate in fungi. Several mycoviruses have been previously reported in Pleurotus ostreatus, including the oyster mushroom spherical virus (OMSV), oyster mushroom isometric virus (OMIV), Pleurotus ostreatus spherical virus (POSV), and Pleurotus ostreatus virus 1 (PoV1). This study was designed to develop a multiplex RT-PCR for simultaneous detection and differentiation of the four P. ostreatus mycoviruses. Four pairs of primers were designed from conserved regions based on the reported sequences and the multiplex RT-PCR products were 672 bp for OMSV, 540 bp for OMIV, 310 bp for POSV, and 200 bp for PoV1. The optimal annealing temperature of the multiplex RT-PCR was 62 °C and the detection limits of the plasmids were 100 fg for OMSV and OMIV and 1 pg for POSV and PoV1. This technique was successfully applied for the detection of OMSV, OMIV, and POSV from different P. ostreatus strains and the plasmid containing the PoV1 sequence. This methodology can serve as a powerful diagnostic tool for the survey of the incidence and epidemiology of the four P. ostreatus mycoviruses, further contributing to the prevention and treatment of mycoviral diseases in P. ostreatus.

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Multiplex Detection of Aspergillus fumigatus Mycoviruses

Cited by 7 publications

References 27 publications

Aspergillus fumigatus and Aspergillosis in 2019

Aspergillus fumigatus and Aspergillosis in 2019

Interactions between Aspergillus fumigatus and Pulmonary Bacteria: Current State of the Field, New Data, and Future Perspective

Multiplex Detection of Pleurotus ostreatus Mycoviruses

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