2017
DOI: 10.1016/j.bios.2017.02.051
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Multiplex detection of extensively drug resistant tuberculosis using binary deoxyribozyme sensors

Abstract: Current diagnostic tools for Mycobacterium tuberculosis (Mtb) have many disadvantages including low sensitivity, slow turnaround times, or high cost. Accurate, easy to use, and inexpensive point of care molecular diagnostic tests are urgently needed for the analysis of multidrug resistant (MDR) and extensively drug resistant (XDR) Mtb strains that emerge globally as a public health threat. In this study, we established proof-of-concept for a novel diagnostic platform (TB-DzT) for Mtb detection and the identifi… Show more

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Cited by 32 publications
(20 citation statements)
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“…We have previously reported the limit of detection (LOD) of a fluorescent BiDz assay targeting Mtb as 10-15 genome equivalents (50-75 fg) (21). In this work, we determined the LOD for the IPDz-substrate based colorimetric assay by PCR amplification from 2-fold serial dilution of Mtb chromosomal DNA (0.125-4ng DNA) and subsequent detection with vBiDz sensors as described in Materials and Methods.…”
Section: Limit Of Detection Of Vbidz-ntm St Assaymentioning
confidence: 99%
See 1 more Smart Citation
“…We have previously reported the limit of detection (LOD) of a fluorescent BiDz assay targeting Mtb as 10-15 genome equivalents (50-75 fg) (21). In this work, we determined the LOD for the IPDz-substrate based colorimetric assay by PCR amplification from 2-fold serial dilution of Mtb chromosomal DNA (0.125-4ng DNA) and subsequent detection with vBiDz sensors as described in Materials and Methods.…”
Section: Limit Of Detection Of Vbidz-ntm St Assaymentioning
confidence: 99%
“…BiDz sensors offer unique advantages over traditional molecular methods. The catalytic feature of the BiDz sensors enables amplification of signal in the presence of a specific analyte resulting in lower limits of detection compared to traditional probe-based molecular detection methods (16,21). Most importantly, careful design of BiDz sensors can enable differentiation of DNA or RNA analytes that differ by a single nucleotide (16,(21)(22)(23).…”
Section: Introductionmentioning
confidence: 99%
“…Исследования в области разработки быстрых, точных и простых в эксплуатации систем диагностики лекарственно устойчивых форм туберкулёза ведутся во многих странах мира [15,16]. В рамках представляемого проекта предполагается разработать и внедрить в практику новую технологическую платформу для экспрессной молекулярной диагностики МЛУ и ШЛУ-ТБ в клинических образцах (мокроте, моче) при использовании системы бинарных дезоксирибозимных сенсоров [17]. Эта технология позволит одновременно обнаруживать ДНК/РНК МЛУ-ТБ непосредственно в клинических образцах и идентифицировать точечные мутации, обусловливающие формирование множественной и широкой лекарственной устойчивости.…”
Section: рис 1 классификация M Tuberculosis на различные линии и сunclassified
“…Notably, the signal appears only in the presence of the DNA/RNA analyte species which are needed for assembling the catalytic BiDZ core. A BiDZ sensor was applied for molecular diagnostics of diseases [21][22][23]. The MaBiDZ system, which includes DNA-functionalized magnetic nanoparticles, was developed for the detection of a specific biomarker inside cancer cells.…”
mentioning
confidence: 99%