Multiplex gRNAs Synergically Enhance Detection of SARS-CoV-2 by CRISPR-Cas12a

Morales-Moreno, Melissa D.; Valdés-Galindo, Erick G.; Reza, Mariana M.; Fiordelisio, Tatiana; Peón, Jörge; Hernández-García, Armando

doi:10.1089/crispr.2022.0074

Cited by 5 publications

(3 citation statements)

References 40 publications

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“…Having PoC ASSURED biosensors can make a difference in controlling a pandemic. During this time, many new biosensors for viral RNA detection were developed, including the use of CRISPR-Cas as optical biosensors (Morales-Moreno et al, 2023), lamp assays, aptamers, antigens, nanoparticles, and plasmon resonance (Mobed and Sepehri The main problems that arise from research and development of biosensors have to do with relying on large, expensive equipment and requiring highly trained personnel. As well as improvement of the signal translation and measurement system so that they can be low-cost, miniaturized devices that can be used by a person at home, in a modest clinic or in the field.…”

Section: Editorial On the Research Topic Methods In Biosensors And Bi...mentioning

confidence: 99%

“…Having PoC ASSURED biosensors can make a difference in controlling a pandemic. During this time, many new biosensors for viral RNA detection were developed, including the use of CRISPR-Cas as optical biosensors ( Morales-Moreno et al, 2023 ), lamp assays, aptamers, antigens, nanoparticles, and plasmon resonance ( Mobed and Sepehri Shafigh, 2021 ), and even adaptations of standardized techniques to improve the sensitivity and speed of a test, as observed in the work of Mao et al on asymmetric stem-loop-mediated isothermal amplification of nucleic acids for DNA diagnostic assays by simple.…”

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confidence: 99%

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Editorial: Methods in biosensors and biomolecular electronics

Fiordelisio

2023

Front. Bioeng. Biotechnol.

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Section: Editorial On the Research Topic Methods In Biosensors And Bi...mentioning

confidence: 99%

“…Having PoC ASSURED biosensors can make a difference in controlling a pandemic. During this time, many new biosensors for viral RNA detection were developed, including the use of CRISPR-Cas as optical biosensors ( Morales-Moreno et al, 2023 ), lamp assays, aptamers, antigens, nanoparticles, and plasmon resonance ( Mobed and Sepehri Shafigh, 2021 ), and even adaptations of standardized techniques to improve the sensitivity and speed of a test, as observed in the work of Mao et al on asymmetric stem-loop-mediated isothermal amplification of nucleic acids for DNA diagnostic assays by simple.…”

mentioning

confidence: 99%

Editorial: Methods in biosensors and biomolecular electronics

Fiordelisio

2023

Front. Bioeng. Biotechnol.

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“…Compared with a single crRNA system, multiplex crRNA strategies have been developed in recent years ( 15 – 17 ). The principle of this method is designing multiple crRNA complementary to the same target sequence.…”

Section: Introductionmentioning

confidence: 99%

Harnessing multiplex crRNA enables an amplification-free/CRISPR-Cas12a-based diagnostic methodology for Nosema bombycis

Zhang,

Zhao,

Cao

et al. 2024

Microbiol Spectr

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Nosema bombycis is transmitted horizontally and vertically from infected female moths to progeny eggs, bringing substantial economic losses to sericulture production. Timely and accurate detection of N. bombycis can effectively block the spread of silkworm pébrine disease, which is of great significance for its prevention and control. In this study, we established a multiplex-crRNA CRISPR/Cas12a nucleic acid amplification-free detection method for N. bombycis .. Specificity and sensitivity assessments confirmed that this method specifically detects N. bombycis without cross-reacting with other pathogens. It can detect N. bombycis at levels as low as 2 pg of genomic DNA. This method could also provide information on pathogen quantification, which will be important for in-field applications and promotions. IMPORTANCE The multiplex-crRNA CRISPR/Cas12a detection method saves hands-on time, reduces the risk of aerosol pollution, and can be directly applied to detecting silkworms infected with Nosema bombycis . This study provides a new approach for the inspection and quarantine of silkworm pébrine disease in sericulture and provides a new method for the detection of other pathogens.

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Multi-faceted CRISPR/Cas technological innovation aspects in the framework of 3P medicine

et al. 2023

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Since 2009, the European Association for Predictive, Preventive and Personalised Medicine (EPMA, Brussels) promotes the paradigm change from reactive approach to predictive, preventive, and personalized medicine (PPPM/3PM) to protect individuals in sub-optimal health conditions from the health-to-disease transition, to increase life-quality of the affected patient cohorts improving, therefore, ethical standards and cost-efficacy of healthcare to great benefits of the society at large. The gene-editing technology utilizing CRISPR/Cas gene-editing approach has demonstrated its enormous value as a powerful tool in a broad spectrum of bio/medical research areas. Further, CRISPR/Cas gene-editing system is considered applicable to primary and secondary healthcare, in order to prevent disease spread and to treat clinically manifested disorders, involving diagnostics of SARS-Cov-2 infection and experimental treatment of COVID-19. Although the principle of the proposed gene editing is simple and elegant, there are a lot of technological challenges and ethical considerations to be solved prior to its broadly scaled clinical implementation. This article highlights technological innovation beyond the state of the art, exemplifies current achievements, discusses unsolved technological and ethical problems, and provides clinically relevant outlook in the framework of 3PM.

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Multiplex gRNAs Synergically Enhance Detection of SARS-CoV-2 by CRISPR-Cas12a

Cited by 5 publications

References 40 publications

Editorial: Methods in biosensors and biomolecular electronics

Editorial: Methods in biosensors and biomolecular electronics

Harnessing multiplex crRNA enables an amplification-free/CRISPR-Cas12a-based diagnostic methodology for Nosema bombycis

Multi-faceted CRISPR/Cas technological innovation aspects in the framework of 3P medicine

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