Multiplex Immunogold Chromatographic Assay for Simultaneous Determination of Macrolide Antibiotics in Raw Milk

Li, Xiangmei; Wen, Kai; Chen, Yiqiang; Wu, Xiaoping; Pei, Xingyao; Wang, Qi; Liu, Ailing; Shen, Jianzhong

doi:10.1007/s12161-015-0130-x

Cited by 32 publications

(8 citation statements)

References 16 publications

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“…The LOD of the ELISA with 0.3 μg/L was lower than that of the developed FPIA with 14.08 μg/L using the same mAb 5B2 in milk [ 28 ]; however, the homogeneous FPIA demands a much shorter time of about 5 min for the detection of ERY in milk, which is urgently required for rapid screening methods. Table S3 shows the LOD and assay time of the developed FPIA compared with typical instrumental analytical methods and immunoassays [ 1 , 12 , 13 , 28 , 36 , 37 , 38 ], demonstrating that the newly established FPIA had the shortest detecting time for ERY under a satisfactory LOD, which confirmed the suitability of this assay for the rapid, accurate, and precise determination of ERY in real samples.…”

Section: Resultsmentioning

confidence: 80%

“…The following supporting information can be downloaded at: , Figure S1: The TLC purification of tracers; Figure S2: Mass spectra of ERY-CMO-4′-AMF(A), ERM-FITC (B), and ERM-SRSC (C); Figure S3: Binding curves for three antibodies with the ERY-CMO-4′-AMF (A), ERM-FITC (B), ERM-AF647 (C), and ERM- SRSC (D); Figure S4: Standard curves for three antibodies with ERY-CMO-4′-AMF (A), ERM-FITC (B), ERM-SRSC (C), and ERM-AF647 (D); Table S1: The IC 50 values and CRs of FPIA; Table S2: The parameters of standard curves in BB, BB with skimmed milk powder, and milk matrix; Table S3: Summary of typically reported immunoassays for the determination of ERY. References [ 1 , 12 , 13 , 28 , 36 , 37 , 38 ] are cited in the Supplementary Materials.…”

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confidence: 99%

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A Robust Homogeneous Fluorescence Polarization Immunoassay for Rapid Determination of Erythromycin in Milk

Duan

Zhang

et al. 2023

Foods

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Erythromycin (ERY) is one of the most common macrolides applied in veterinary medicine to treat diseases or as a feed additive for animal growth promotion. Long-term irrational use of ERY could lead to residues in animal-derived food and the emergence of drug-resistant strains, posing potential threats to human health. In this study, a highly sensitive, specific, robust, and rapid fluorescence polarization immunoassay (FPIA) for the determination of ERY in milk has been described. Herein, to achieve high sensitivity, five tracers of ERY with different fluorescein structures were synthesized and paired with three monoclonal antibodies (mAbs). Under the optimized conditions, the combination of mAb 5B2 and tracer ERM-FITC achieved the lowest IC50 value in the FPIA with 7.39 μg/L for ERM. The established FPIA was used to detect ERY in milk, revealing a limit of detection (LOD) of 14.08 μg/L with recoveries of 96.08–107.77% and coefficients of variations (CVs) of 3.41–10.97%. The total detection time of the developed FPIA was less than 5 min from the addition of samples to the result readout. All the above results showed that the proposed FPIA in this study was a rapid, accurate, and simple method for the screening of ERY in milk samples.

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Section: Resultsmentioning

confidence: 80%

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confidence: 99%

A Robust Homogeneous Fluorescence Polarization Immunoassay for Rapid Determination of Erythromycin in Milk

Duan

Zhang

et al. 2023

Foods

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“…CGNPs were prepared as described previously with some modifications. 18 Briefly, 100 mL of ultrapure water was heated to boiling under stirring. Then, 1.0 mL of 1% chloroauric acid solution (w/v) was added.…”

Section: Methodsmentioning

confidence: 99%

Development of a monoclonal antibody-based lateral flow immunoassay for the detection of benzoic acid in liquid food

Zhao,

Ding,

Pei

et al. 2023

Anal. Methods

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“…CGNPs were prepared as described previously with some modi cations (Li et al 2015). Brie y, 100 mL of ultrapure water was heated to boiling under stirring.…”

Section: Preparation Of Cgnpsmentioning

confidence: 99%

Development of a Sensitive Monoclonal Antibody-Based Lateral Flow Immunoassay for the Detection of Benzoic Acid in Liquid Food

Zhao

Ding

Pei

et al. 2023

Preprint

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To monitor benzoic acid (BA) residues in liquid food samples, a monoclonal antibody (mAb)-based lateral flow immunoassay (LFA) was developed in this study. First, 2-aminobenzoic acid (2-AA), 3-aminobenzoic acid (3-AA), and 4-aminobenzoic acid (4-AA) were conjugated to BSA and used as immunogens. After cell fusion, mAb 6D8 from 4-AA-BSA performed best with an IC50 value of 0.21 mg/L using 3-AA-OVA as heterogeneous antigen, which represented a 3.4-fold improvement compared with the homogeneous antigen 4-AA-BSA. Subsequently, eight kinds of CGNPs with sizes varying from 20.94 nm to 90.00 nm were synthesized for screening the suitable size to develop a sensitive LFA. Finally, a sensitive LFA based on colloidal gold (23.27nm) nanoparticles was developed for screening BA with a cut-off value of 4 mg/L, which could meet the requirement of BA detection in milk, Fanta, Sprite, Coca-Cola, and Stmart samples.

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Multiplex Immunogold Chromatographic Assay for Simultaneous Determination of Macrolide Antibiotics in Raw Milk

Cited by 32 publications

References 16 publications

A Robust Homogeneous Fluorescence Polarization Immunoassay for Rapid Determination of Erythromycin in Milk

A Robust Homogeneous Fluorescence Polarization Immunoassay for Rapid Determination of Erythromycin in Milk

Development of a monoclonal antibody-based lateral flow immunoassay for the detection of benzoic acid in liquid food

Development of a Sensitive Monoclonal Antibody-Based Lateral Flow Immunoassay for the Detection of Benzoic Acid in Liquid Food

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