Multiplex isothermal helicase-dependent amplification assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae

Doseeva, Victoria; Forbes, Thomas; Wolff, John S.; Khripin, Yuri; O'Neil, Dominic; Rothmann, Thomas; Nazarenko, Irina

doi:10.1016/j.diagmicrobio.2011.08.021

Cited by 35 publications

(20 citation statements)

References 28 publications

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“…Our search yielded fourteen proof of concept articles for new POC diagnostics [ 38 – 51 ]. Eleven reports introduced CT diagnostics [ 38 – 48 ], five introduced NG diagnostics [ 39 , 47 – 50 ], and two introduced TV diagnostics [ 47 , 51 ]. The diagnostic methods and performance, if provided, for those novel tests are summarized in Tables 4 – 6 .…”

Section: Resultsmentioning

confidence: 99%

A Systematic Review of Point of Care Testing forChlamydia trachomatis,Neisseria gonorrhoeae, andTrichomonas vaginalis

Cortina

Bristow

Davey

et al. 2016

Infectious Diseases in Obstetrics and Gynecology

107

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Objectives. Systematic review of point of care (POC) diagnostic tests for sexually transmitted infections: Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), and Trichomonas vaginalis (TV). Methods. Literature search on PubMed for articles from January 2010 to August 2015, including original research in English on POC diagnostics for sexually transmitted CT, NG, and/or TV. Results. We identified 33 publications with original research on POC diagnostics for CT, NG, and/or TV. Thirteen articles evaluated test performance, yielding at least one test for each infection with sensitivity and specificity ≥90%. Each infection also had currently available tests with sensitivities <60%. Three articles analyzed cost effectiveness, and five publications discussed acceptability and feasibility. POC testing was acceptable to both providers and patients and was also demonstrated to be cost effective. Fourteen proof of concept articles introduced new tests. Conclusions. Highly sensitive and specific POC tests are available for CT, NG, and TV, but improvement is possible. Future research should focus on acceptability, feasibility, and cost of POC testing. While pregnant women specifically have not been studied, the results available in nonpregnant populations are encouraging for the ability to test and treat women in antenatal care to prevent adverse pregnancy and neonatal outcomes.

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Section: Resultsmentioning

confidence: 99%

A Systematic Review of Point of Care Testing forChlamydia trachomatis,Neisseria gonorrhoeae, andTrichomonas vaginalis

Cortina

Bristow

Davey

et al. 2016

Infectious Diseases in Obstetrics and Gynecology

107

View full text Add to dashboard Cite

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“…HDA amplification usually takes 60-120 min, at least for low-copy numbers, but possible modifications for optimization can be done [45]. Although only a single set of primers is needed for HDA, few multiplex HDA applications have been developed so far such as a duplex-detection of pathogens N. gonorrhoeae and Staphylococcus aureus by combining HDA with microarray-based detection [46], and a 4-plex detection assay for four different genes from the same organisms [47]. HDA is compatible with diverse detection mechanisms like fluorescent detection [48] or electrochemical DNA detection with gold nanoparticles [49].…”

Section: Helicase-dependent Amplificationmentioning

confidence: 99%

Molecular isothermal techniques for combating infectious diseases: towards low-cost point-of-care diagnostics

Paz

Brotons

Muñoz-Almagro³

2014

Expert Review of Molecular Diagnostics

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Nucleic acid amplification techniques such as PCR have facilitated rapid and accurate diagnosis in central laboratories over the past years. PCR-based amplifications require high-precision instruments to perform thermal cycling reactions. Such equipment is bulky, expensive and complex to operate. Progressive advances in isothermal amplification chemistries, microfluidics and detectors miniaturisation are paving the way for the introduction and use of compact 'sample in-results out' diagnostic devices. However, this paradigm shift towards decentralised testing poses diverse technological, economic and organizational challenges both in industrialized and developing countries. This review describes the landscape of molecular isothermal diagnostic techniques for infectious diseases, their characteristics, current state of development, and available products, with a focus on new directions towards point-of-care applications.

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“… 23 , 24 At the same time, HDA offers the advantages of PCR technology, such as high specificity and sensitivity, as well as the capability for multiplex detection. 25 , 26 Furthermore, the relative simplicity of the reaction makes HDA an appealing method for incorporation into portable, battery-operated microfluidic lab-on-the-chip systems. 27 − 30 For instance, HDA-employing microfluidic devices that integrate nucleic acid extraction, amplification, and detection steps in a single device have been described.…”

Section: Introductionmentioning

confidence: 99%

“…Until now, however, HDA has been mainly used for pathogen detection in clinical diagnostics. 26 , 32 − 35 …”

Section: Introductionmentioning

confidence: 99%

A Complementary Isothermal Amplification Method to the U.S. EPA Quantitative Polymerase Chain Reaction Approach for the Detection of Enterococci in Environmental Waters

Kolm

Martzy

Brunner

et al. 2017

Environ. Sci. Technol.

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We report a novel molecular assay, based on helicase-dependent amplification (HDA), for the detection of enterococci as markers for fecal pollution in water. This isothermal assay targets the same Enterococcus 23S rRNA gene region as the existing quantitative polymerase chain reaction (qPCR) assays of U.S. Environmental Protection Agency Methods 1611 and 1609 but can be entirely performed on a simple heating block. The developed Enterococcus HDA assay successfully discriminated 15 enterococcal from 15 non-enterococcal reference strains and reliably detected 48 environmental isolates of enterococci. The limit of detection was 25 target copies per reaction, only 3 times higher than that of qPCR. The applicability of the assay was tested on 30 environmental water sample DNA extracts, simulating a gradient of fecal pollution. Despite the isothermal nature of the reaction, the HDA results were consistent with those of the qPCR reference. Given this performance, we conclude that the developed Enterococcus HDA assay has great potential as a qualitative molecular screening method for resource-limited settings when combined with compatible up- and downstream processes. This amplification strategy can pave the way for developing a new generation of rapid, low-cost, and field-deployable molecular diagnostic tools for water quality monitoring.

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Multiplex isothermal helicase-dependent amplification assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae

Cited by 35 publications

References 28 publications

A Systematic Review of Point of Care Testing forChlamydia trachomatis,Neisseria gonorrhoeae, andTrichomonas vaginalis

A Systematic Review of Point of Care Testing forChlamydia trachomatis,Neisseria gonorrhoeae, andTrichomonas vaginalis

Molecular isothermal techniques for combating infectious diseases: towards low-cost point-of-care diagnostics

A Complementary Isothermal Amplification Method to the U.S. EPA Quantitative Polymerase Chain Reaction Approach for the Detection of Enterococci in Environmental Waters

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