Multiplex monitoring of Alzheimer associated miRNAs based on the modular logic circuit operation and doping of catalytic hairpin assembly

Ebrahimi, Ali; Ravan, Hadi; Mehrabani, Mehrnaz

doi:10.1016/j.bios.2020.112710

Cited by 33 publications

(12 citation statements)

References 23 publications

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“…Our group recently showed that melatonin interacts with DAPK1 to inhibit protein expression, but a loss of melatonin might not be sufficient to augment the protein stabilization of DAPK1, which indicates that the expression of DAPK1 is modulated by multiple mechanisms, including miRNAs, in AD [ 14 , 21 ]. By employing a variety of miRNA online databases, we identified hsa-miR-143-3p as an attractive and novel biomarker for early AD diagnosis [ 34 , 35 , 36 , 37 ]. Through validation using dual-luciferase reporter assays, we provide the first demonstration showing that human DAPK1 is a direct target of hsa-miR-143-3p.…”

Section: Discussionmentioning

confidence: 99%

“…MicroRNA-143 (miR-143) is part of a bicistronic cluster composed of miR-143 and miR-145, which is located on chromosome 5 position 33 (5q33) in the human genome and has been implicated in the carcinogenesis [ 32 , 33 ]. Recent findings showing the diagnostic potential of hsa-miR-143-3p for AD have inspired many researchers [ 34 , 35 , 36 , 37 ]. However, the hsa-miR-143-3p levels in adult brains and the molecular mechanisms of hsa-miR-143-3p in AD pathogenesis remain unclear.…”

Section: Introductionmentioning

confidence: 99%

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miR-143-3p Inhibits Aberrant Tau Phosphorylation and Amyloidogenic Processing of APP by Directly Targeting DAPK1 in Alzheimer’s Disease

Wang

Shui

Mei

et al. 2022

IJMS

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The neuropathology of Alzheimer’s disease (AD) is characterized by intracellular aggregation of hyperphosphorylated tau and extracellular accumulation of beta-amyloid (Aβ). Death-associated protein kinase 1 (DAPK1), as a novel therapeutic target, shows promise for the treatment of human AD, but the regulatory mechanisms of DAPK1 expression in AD remain unclear. In this study, we identified miR-143-3p as a promising candidate for targeting DAPK1. miR-143-3p directly bound to the 3′ untranslated region of human DAPK1 mRNA and inhibited its translation. miR-143-3p decreased tau phosphorylation and promoted neurite outgrowth and microtubule assembly. Moreover, miR-143-3p attenuated amyloid precursor protein (APP) phosphorylation and reduced the generation of Aβ40 and Aβ42. Furthermore, restoring DAPK1 expression with miR-143-3p antagonized the effects of miR-143-3p in attenuating tau hyperphosphorylation and Aβ production. In addition, the miR-143-3p levels were downregulated and correlated inversely with the expression of DAPK1 in the hippocampus of AD patients. Our results suggest that miR-143-3p might play critical roles in regulating both aberrant tau phosphorylation and amyloidogenic processing of APP by targeting DAPK1 and thus offer a potential novel therapeutic strategy for AD.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

miR-143-3p Inhibits Aberrant Tau Phosphorylation and Amyloidogenic Processing of APP by Directly Targeting DAPK1 in Alzheimer’s Disease

Wang

Shui

Mei

et al. 2022

IJMS

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“…8,9 Thanks to the feature of easy preparation, gold nanoparticles (AuNPs) are frequently adopted in bCHA-based colorimetric approaches, in which TWJ products could induce the aggregation of AuNPs accompanied with color changes from red to blue. These methods could improve the sensitivity to the pM level for DNA or RNA detection [10][11][12] and 10 cells for cancer cell determination. 13 Branched CHA with TWJ products has also been applied in fluorescence assays for higher sensitivity, since TWJ structures can load more fluorescent signal reporter molecules than DNA duplexes through typical CHA.…”

Section: Branched Chamentioning

confidence: 99%

Section: Newly Developed Cha Strategiesmentioning

confidence: 99%

Recent advances of catalytic hairpin assembly and its application in bioimaging and biomedicine

et al. 2022

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“…DNA nanotechnology has developed rapidly in recent years, and as its main material, DNA molecules have been widely used in DNA computing [ 1 , 2 , 3 ], artificial intelligence [ 4 , 5 ], disease diagnosis [ 6 ], and other fields [ 7 ]. The DNA molecule follows the principle of Watson–Crick complementary base pairing, which has powerful parallel computing capabilities and excellent data storage capabilities, so it is expected to perform computational operations.…”

Section: Introductionmentioning

confidence: 99%

DNA Matrix Operation Based on the Mechanism of the DNAzyme Binding to Auxiliary Strands to Cleave the Substrate

Liu

Zhou

et al. 2021

Biomolecules

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Numerical computation is a focus of DNA computing, and matrix operations are among the most basic and frequently used operations in numerical computation. As an important computing tool, matrix operations are often used to deal with intensive computing tasks. During calculation, the speed and accuracy of matrix operations directly affect the performance of the entire computing system. Therefore, it is important to find a way to perform matrix calculations that can ensure the speed of calculations and improve the accuracy. This paper proposes a DNA matrix operation method based on the mechanism of the DNAzyme binding to auxiliary strands to cleave the substrate. In this mechanism, the DNAzyme binding substrate requires the connection of two auxiliary strands. Without any of the two auxiliary strands, the DNAzyme does not cleave the substrate. Based on this mechanism, the multiplication operation of two matrices is realized; the two types of auxiliary strands are used as elements of the two matrices, to participate in the operation, and then are combined with the DNAzyme to cut the substrate and output the result of the matrix operation. This research provides a new method of matrix operations and provides ideas for more complex computing systems.

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Multiplex monitoring of Alzheimer associated miRNAs based on the modular logic circuit operation and doping of catalytic hairpin assembly

Cited by 33 publications

References 23 publications

miR-143-3p Inhibits Aberrant Tau Phosphorylation and Amyloidogenic Processing of APP by Directly Targeting DAPK1 in Alzheimer’s Disease

miR-143-3p Inhibits Aberrant Tau Phosphorylation and Amyloidogenic Processing of APP by Directly Targeting DAPK1 in Alzheimer’s Disease

Recent advances of catalytic hairpin assembly and its application in bioimaging and biomedicine

DNA Matrix Operation Based on the Mechanism of the DNAzyme Binding to Auxiliary Strands to Cleave the Substrate

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