Multiplex PCR assay for the detection of five meat species forbidden in Islamic foods

Ali, Eaqub; Razzak, Md. Abdur; Hamid, Sharifah Bee Abd; Rahman, Mohd Muhiden Abd; Amin, Md. Al; Rashid, Nur Raifana Abd; Asing,

doi:10.1016/j.foodchem.2014.12.098

Cited by 183 publications

(129 citation statements)

References 38 publications

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“…Such slaughters have been reported previously in Brazil (MATHIAS, 2010) and misrepresent the production indices related to beef and buffalo meat consumption. Several methods of detecting adulteration have been reported, and many researchers have highlighted multiplex PCR as a fast, affordable and simple method for species screening in food (ALI et al, 2015;KARABASANAVAR et al, 2013). These researchers have been able to identify different species within the same reaction, using methods and producing results similar to those reported in this study.…”

Section: Discussionmentioning

confidence: 99%

“…CHENG et al (2014) stated that DNA-based methods are used more frequently, especially due tothe stability and ubiquity of DNA in tissues. Polymerase chain reaction (PCR) is an efficient method for detecting adulteration, and this method has already been usedto detect adulteration in meat products (DAGUER et al, 2010;OLIVEIRA et al, 2015;KARABASANAVAR et al, 2013;ALI et al, 2015). AMARAL et al (2015) developed a PCR protocol capable of identifying the presence of six animal species in the same reaction.…”

Section: Introductionmentioning

confidence: 99%

“…In tests involving commercial sausages, the authors detected labeling inconsistencies regarding the absence of declared meats. Although, several studies have demonstrated the efficiency of PCR for research regarding the adulteration of meat products (OLIVEIRA et al, 2015;KARABASANAVAR et al, 2013;ALI et al, 2015), few articles have reported on commercially available ground meat samples.…”

Section: Introductionmentioning

confidence: 99%

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Brazilian ground beef authentication by multiplex polymerase chain reaction

Oliveira

Pedroso²,

Cardilli³

et al. 2018

Cienc. Rural

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The aim of the present study was to assess the efficacy ofmultiplex PCR in detecting the adulterationof commercially available ground beefvia addition and/orsubstitution ofground buffalo meat. Experimentally adulterated ground beefsamples were prepared in triplicate, and dilutions of DNA from Bos taurus and Bubalusbubalis were prepared to determine the detection limit of the method. Concurrently, 91 ground meatsamples sold as “ground beef” were collected from differentstores in northern Brazil andanalyzed bymultiplex PCR. Buffalo DNA was detected in 17.5% of the collected ground meat samples.Our results showed that multiplex PCR is an efficient method for detectingthe incorporation of groundbuffalo meatatpercentages ranging from 10 to 100% and the incorporation of beef at percentages ranging from0.1 to 100% intoground meat samples.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Brazilian ground beef authentication by multiplex polymerase chain reaction

Oliveira

Pedroso²,

Cardilli³

et al. 2018

Cienc. Rural

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“…According to the authors, the target meat species could be detected at a level of 1%. Similarly, Ali et al (2015) and Ilhak and Arslan (2007) were able to determine the origin of several meat species (beef, sheep, pork, goat, horse, cat, dog, monkey, and rat) by multiplex PCR assays, using amplified species-specific fragments of the mitochondrial and cytochrome b genes. Currently, a new challenge in the identification of meat origin in foods has emerged, which involves the development of specific nanoparticle-based probes, capable of detecting several-fold shorter DNA target sequences found in highly processed products .…”

Section: Introductionmentioning

confidence: 99%

Effectual Gold Nanoprobe Sensor for Screening Horse Adulteration in Meat Products

Houhoula¹,

Kouzilou²,

Tzogias³

et al. 2017

JFR

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A gold nanoparticle (AuNP) probe strategy for testing meat authenticity was developed, which relies on the colorimetric differentiation of a particular DNA sequence, due to the differential aggregation profiles exhibited by the AuNPs in the presence or absence of specific target hybridization. Gold nanoparticles were conjugated with thiolated oligonucleotides for specifically identifying a 69 bp fragment of the horse cytochrome b gene. In the presence of a complementary target preventing aggregation of the AuNPs when acid was added, the reaction mixtures retained the original pink colouration of the colloidal particles, whereas they turned purple in the opposite event. Fresh meatballs, prepared using pure bovine meat, were used as blanks, producing a purplish coloured solution with a peak at ≥570nm. Horse meat was used as positive control and the pink colour obtained after hybridization exhibited maximum absorption at 524 nm. Both the specificity and sensitivity of the tests performed were 100%. Visual observations and spectroscopic data indicated that the coloration produced by the AuNPs (positive-pink, negative-purple) was very stable, showing no change under normal laboratory conditions. The use of AuNPs for the colorimetric detection of DNA targets from undeclared species in meat products provides an inexpensive and easy-to-perform alternative to common molecular assays. The technology described here can be further developed to accommodate detection of many cases of adulteration and fraudulent practices.

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“…Furthermore, multiplex PCR assays can be used with species-specific primers to multiple target detection at the same time. This technique provides reduction in time and cost of assays (Ali et al, 2015). However, there is no study about gelatin differentiating methods conducted with multiplex PCR assays.…”

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confidence: 99%

Origin Determination and Differentiation of Gelatin Species of Bovine, Porcine, and Piscine through Analytical Methods

Eryılmaz

Işık

Demircan

et al. 2017

Turkish JAF Sci.Tech.

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Gelatin origin determination has been a crucial issue with respect to religion and health concerns. It is necessary to analyze the origin of gelatin with reliable methods to ensure not only consumer choices but also safety and legal requirements such as labeling. There are many analytical methods developed for detection and/or quantification of gelatin from different sources including bovine, porcine and piscine. These analytical methods can be divided into physicochemical, chromatographic, immunochemical, spectroscopic and molecular methods. Moreover, computational methods have been used in some cases consecutively to ensure sensitivity of the analytical methods. Every method has different advantages and limitations due to their own principles, applied food matrix and process conditions of material. The present review intends to give insight into novel analytical methods and perspectives that have been developed to differentiate porcine, bovine and piscine gelatins and to establish their authenticity. Almost every method can be succeeded in origin determination; however, it is a matter of sensitivity in that some researches fail to ensure sufficient differentiation. IntroductionGelatin is a water soluble protein obtained by hydrolysis of collagen to several high molecular weight polypeptides. While the main component of the gelatin is protein (85-92%), it also contains mineral salts and moisture (Baziwane and He, 2003;Schrieber and Gareis, 2007). Gelatin has a great importance in pharmaceutical, cosmetic and especially food industry with its well-known functional properties such as gel formation in jellies, foam formation and stabilization in ice cream, emulsion stabilization in meat products, syneresis stabilization in yogurt (Nhari et al., 2012). Gelatin was produced approximately 326.000 metric tons in 2008 worldwide, and it is estimated to be consumed 395.840 metric tons by the year of 2017 according to Global Industry Analysts, Inc. which is one of the largest and reputed market research companies (Anonymous, 2016).The raw materials used in gelatin production are mainly from bovine and porcine sources: 80% from pig skin, 15% from cattle hide split, the remaining 5 % from bones, fish and other sources (GME, 2016). Gelatin origin determination has been a matter of paramount importance for Muslims, Hindus, Jews, vegetarians and vegans, in that, in Islam and Judaism, porcine derivatives are prohibited to consume, whereas in Hinduism, cow can neither be slaughtered nor consumed. Vegetarians and vegans prefer to eat animal-free foods (Boran et al., 2010;Nhari et al., 2012). Moreover, bovine gelatin has been restricted for human consumption due to Bovine Spongiform Encephalopathy (BSE) outbreak in 1986 in England and other countries (Venien and Levieux, 2005). Therefore, it is necessary to analyze the origin of gelatin with a reliable method. As far as it is concerned; physiochemical, chromatographic, immunochemical, spectroscopic and molecular methods have been developed to determine and differentiate gelatin o...

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Multiplex PCR assay for the detection of five meat species forbidden in Islamic foods

Cited by 183 publications

References 38 publications

Brazilian ground beef authentication by multiplex polymerase chain reaction

Brazilian ground beef authentication by multiplex polymerase chain reaction

Effectual Gold Nanoprobe Sensor for Screening Horse Adulteration in Meat Products

Origin Determination and Differentiation of Gelatin Species of Bovine, Porcine, and Piscine through Analytical Methods

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