Multiplex PCR-based DNA array for simultaneous detection of three human herpesviruses, EVB, CMV and KSHV

Multiplex polymerase chain reaction for six herpesviruses after hematopoietic stem cell transplantation

“…The regions amplified by multiplex PCR and RQ‐PCR are different, but neither of them are repeated sequences (a single copy in one genome). Difference in sensitivities in a multiplex PCR was also reported in other studies 19,20 . The sensitivity of RQ‐PCR is also variable depending on the systems used 21 .…”

Viral Chip Technology in Genomic Medicine

“…Consensus primers for the amplifi cation of all members of each genus and sequences that are specifi c for the identifi cation of each virus species were selected from the sequence alignments of each target gene. A multiplex PCR-based DNA array for EBV, CMV, and Kaposi 's sarcoma-associated herpesvirus (KSHV) may serve as a rapid and reliable diagnostic tool for clinical applications ( Fujimuro et al, 2006 ).…”

Evidence of inability of human cytomegalovirus to reactivate Kaposi's sarcoma-associated herpesvirus from latency in body cavity-based lymphocytes

“…Indeed, KSHV and HCMV are often detected simultaneously in the same patient, more importantly both viruses can be isolated from blood cells (3, 9, 12, 15, 16, 22, 29). HCMV IE1 can inhibit histone deacetylase (HDAC) activity (21, 27), which mimics the function of trichostatin A (TSA) or sodium butyrate (NaB); it is reasonable to speculate that HCMV can cause the lytic switch of KSHV infection if HCMV infected the cells that harbor the KSHV genome.…”