Multiplex PCR for detection of three exfoliative toxin serotype genes inStaphylococcus aureus

Abstract

Rapid and specific detection of exfoliative toxin (ET)-producing Staphylococcus aureus strains by multiplex polymerase chain reaction (PCR) was used for identification of exfoliative toxin genes in a diverse set of 115 clinical S. aureus strains isolated in 14 Czech cities between 1998 and 2004. Fifty-nine wild-type ET-positive isolates of which 40 strains were the causative agents of toxic epidermolysis in neonates were classified into 4 PCR types. The genes coding for ETA, ETB or ETD were not detected in any of non-ET-producing isolates. The PCR method using the multiplex and specific primer set was shown to be reliable in rapid identification of the exfoliative toxin producing S. aureus and can be used as a convenient tool for hospital epidermolytic infection control.

Characteristics and distribution of plasmids in a clonally diverse set of methicillin-resistant Staphylococcus aureus strains

“…The most often described plasmid-borne staphylococcal virulence genes, that is, the enterotoxin D gene sed and exfoliative toxin B gene etb, were determined by PCR as described previously (Johnson et al 1991;Růžičková et al 2005). The production of exfoliative toxin B and enterotoxin D was assayed by using the Reverse Passive Latex Agglutination Kits EXT-RPLA and SET-RPLA (Denka Seiken).…”

Investigating prevalence of pathogenic genes (ETA and TSST-1) in Staphylococcus aureus isolated from different wards of the hospitals by PCR method

“…In fact, another research was done in Czech and showed that 48 strains out of 59 isolated strains were carriers of eta gene. In this research, 40 strains were isolated from sick children, 10 strains from nurses' hands and 5 strains from the equipment [20] Another research was done in 2005; in this research 103 strains of Staphylococcus aureus were isolated from community, hospital and patients with granulomatous. 12 strains out of 51 strains isolated from community, 5 strains out of 34 strains isolated from hospital and 4 strains out of 16 strains isolated from patients with granulomatous reported presence of tst gene [21]In the present research, 29 strains out of 35 strains isolated from the patients, 5 strains out of 6strains isolated from the personnel and 15 strains out of 20 strains isolated from the equipment included tst gene.…”

Necrotizing pneumonia due to clonally diverse Staphylococcus aureus strains producing Panton-Valentine leukocidin: the Czech experience

“…All isolates were genotyped by pulsed-field gel electrophoresis (PFGE) after Sma I digestion, staphylococcal protein A gene ( spa ) typing using StaphType software v. 2.2.1 (Ridom, Germany), multilocus sequence typing (MLST) using BioNumerics v. 6.6 (Applied Maths, Belgium) MLST online plugin, agr group determination [10], SCC mec typing [11], plasmid content analysis [12], and prophage typing [13]. The genes for PVL [14], leukocidin luk ED [15], staphylococcal enterotoxins [16], enterotoxin gene cluster (EGC) [17], exfoliative toxin genes [18], immune evasion cluster (IEC) [19], and arginine catabolic mobile element (ACME) [20] were screened using polymerase chain reaction (PCR) or multiplex PCR.…”