Multiplex PCR for the diagnosis of red sea bream iridoviruses isolated in Korea

Jeong, Joon Bum; Park, Kyung Hyun; Kim, Ho Yeoul; Hong, Seonki; Kim, Ki Hong; Chung, Joon-Ki; Komisar, Jack; Jeong, Hyun Do

doi:10.1016/j.aquaculture.2004.02.011

Cited by 15 publications

(7 citation statements)

References 24 publications

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“…The use of monoclonal antibody M10 is described in the Manual of Diagnostic Methods for Aquatic Animals published by the World Organization for Animal Health (OIE) and is applicable to both RSIV and ISKNV. Other rapid and more sensitive confirmatory diagnostic methods, such as PCR [ 12 , 15 , 16 , 17 , 18 ], have also been developed. Unfortunately, one of the PCR tests [ 17 ] was subsequently shown to be incorrect since it detected a contaminant bacterial species closely related to Acholeplasma laidlawii rather than RSIV.…”

Section: Diagnostic Methods For Rsivdmentioning

confidence: 99%

Megalocytiviruses

Kurita

Nakajima

2012

Viruses

231

218

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The genus Megalocytivirus, represented by red sea bream iridovirus (RSIV), the first identified and one of the best characterized megalocytiviruses, Infectious spleen and kidney necrosis virus (ISKNV), the type species of the genus, and numerous other isolates, is the newest genus within the family Iridoviridae. Viruses within this genus are causative agents of severe disease accompanied by high mortality in multiple species of marine and freshwater fish. To date outbreaks of megalocytivirus-induced disease have occurred primarily in south-east Asia and Japan, but infections have been detected in Australia and North America following the importation of infected ornamental fish. The first outbreak of megalocytiviral disease was recorded in cultured red sea bream (Pagrus major) in Japan in 1990 and was designated red sea bream iridovirus disease (RSIVD). Following infection fish became lethargic and exhibited severe anemia, petechiae of the gills, and enlargement of the spleen. Although RSIV was identified as an iridovirus, sequence analyses of RSIV genes revealed that the virus did not belong to any of the four known genera within the family Iridoviridae. Thus a new, fifth genus was established and designated Megalocytivirus to reflect the characteristic presence of enlarged basophilic cells within infected organs. Indirect immunofluorescence tests employing recently generated monoclonal antibodies and PCR assays are currently used in the rapid diagnosis of RSIVD. For disease control, a formalin-killed vaccine was developed and is now commercially available in Japan for several fish species. Following the identification of RSIV, markedly similar viruses such as infectious spleen and kidney necrosis virus (ISKNV), dwarf gourami iridovirus (DGIV), turbot reddish body iridovirus (TRBIV), Taiwan grouper iridovirus (TGIV), and rock bream iridovirus (RBIV) were isolated in East and Southeast Asia. Phylogenetic analyses of the major capsid protein (MCP) and ATPase genes indicated that although these viruses shared considerable sequence identity, they could be divided into three tentative species, represented by RSIV, ISKNV and TRBIV, respectively. Whole genome analyses have been reported for several of these viruses. Sequence analysis detected a characteristic difference in the genetic composition of megalocytiviruses and other members of the family in reference to the large and small subunits of ribonucleotide reductase (RR-1, RR‑2). Megalocytiviruses contain only the RR-2 gene, which is of eukaryotic origin; whereas the other genera encode both the RR-1 and RR-2 genes which are thought to originate from Rickettsia-like α-proteobacteria.

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Section: Diagnostic Methods For Rsivdmentioning

confidence: 99%

Megalocytiviruses

Kurita

Nakajima

2012

Viruses

231

218

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“…(2002), this virus caused mortality in 31 species of cultured marine fish, mainly including the orders Perciformers and Pleuronectiformes. Jung and Oh (2000) reported the presence of RSIV in striped beak perch [ Oplegnathus fasciatus (Temminck & Schlegel 1844)], whereas Jeong et al. (2004) detected it in sea perch ( Lateolabrax sp.…”

Section: Epizootics Of Megalocytivirusmentioning

confidence: 99%

Megalocytivirus infection in fish

Subramaniam

Shariff

Omar

et al. 2012

Reviews in Aquaculture

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Megalocytivirus is a newly discovered DNA‐virus from the Iridoviridae family that possesses icosahedral symmetry and has a size range of 140–200 nm diameter. Based on genetic differences, Megalocytivirus is divided into three major groups; infectious spleen and kidney necrosis virus which is reported to cause disease in numerous marine and freshwater fish species, red sea bream iridovirus that mainly infects red sea bream [Pagrus major (Temminck & Schlegel 1843)] and turbot reddish body iridovirus that is reported to infect Asian flounder species. Megalocytivirus has not been studied as extensively as other genera in the Iridoviridae family until recently; these viruses have received the most attention in research due to the wider host range and cause significant mortality and economic losses in the aquaculture industry. This review provides information on taxonomy, viral properties, epizootic, pathology and diagnostics of Megalocytivirus infection in fish.

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“…In Japan, a serious disease of red sea bream (Pagrus major) has been attributed to an iridovirus that has been named red sea bream iridovirus (RSIV). RSIV disease causes mass mortalities and huge economic losses not only in cultured red sea bream, but also in 30 other cultured marine fish species (22,24,25,29,60).…”

mentioning

confidence: 99%

Transcription Program of Red Sea Bream Iridovirus as Revealed by DNA Microarrays

Dang

Yasuike

Hirono

et al. 2005

J Virol

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Although the three classes of transcripts were distributed throughout the RSIV genome, E transcripts appeared to cluster in at least six discrete regions and L transcripts appeared to originate from seven discrete regions. The microarray data were statistically confirmed by using a t test, and were also clustered into groups based on similarity in the gene expression patterns by using a cluster program.

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Multiplex PCR for the diagnosis of red sea bream iridoviruses isolated in Korea

Cited by 15 publications

References 24 publications

Megalocytiviruses

Megalocytiviruses

Megalocytivirus infection in fish

Transcription Program of Red Sea Bream Iridovirus as Revealed by DNA Microarrays

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