Multiplex PCR Technique for Simultaneous Detection and genotyping of E. granulosus, E. multilocularis, and other Taeniidae in some intermediate hosts in Erbil Province

Abstract

One of the most significant helminthozoonosis in Iraq and around the world is cystic echinococcosis, which occur by the etiologic agent of Echinococcus granulosus and it’s distinguished by considerable intra-specific variability (genotypes G1–G10). DNA was extracted from 48 isolated cysts (25 sheep, 12 goats and 11 human) and used as templates to amplify using Trachsel method to differentiate Taeniide (Echinococcus spp. and/ or Taenia spp.) and mitochondrial cytochrome c oxidase subunit one (COX1) gene for genotyping. The PCR products were sequenced, and sequence analysis was used to further evaluate the data. Out of 48 separated of the host cysts, 36 isolates displayed the G1 genotype (100%), and in 12 samples, a nucleotide substitution at position 58 (T→C) results in polymorphism (99.9%). The G1 is considered that the most contagious and prevalent genotype of E. granulosus in the world. Our investigation revealed that, a single genotype that may be in charge of the disease's infectivity in sheep, goats, and human as well as its persistence in endemic areas. these epidemiological findings could be guided the successful hydatidosis control measures in Erbil Provence