Multiplex Quantification Identifies Novel Exercise-regulated Myokines/Cytokines in Plasma and in Glycolytic and Oxidative Skeletal Muscle

Little, Hannah C.; Tan, Stephen; Cali, Francesca M.; Rodriguez, Susana; Lei, Xia; Wolfe, Andrew; Hug, Christopher; Wong, G. William

doi:10.1074/mcp.ra118.000794

Cited by 24 publications

(26 citation statements)

References 57 publications

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“…Maximal sprint and endurance exercise tests were performed as we previously described (67). Briefly, 12-wk-old mice fed a standard chow diet were first subjected to the endurance exercise test.…”

Section: Maximal Exercise Capacitymentioning

confidence: 99%

“…Mice used in this experiment were fed a standard chow diet. Mice were acclimated to the treadmill as previously described, but with the addition of a fourth acclimation day in which mice ran on the treadmill at 14 m/min for 5 min at a 10°incline (67). The endurance exercise run was performed on the fifth day, when the mice were 11 wk of age.…”

Section: Recovery After Endurance Exercisementioning

confidence: 99%

“…A shock grid at the back of the treadmill encouraged mice to continue running by administering a mild shock each time they stepped off the belt. If any mice met the predetermined criteria for exhaustion before the end of the run, they were removed from the treadmill and their data were omitted from analysis (67). The experimenter was blinded with respect to genotype during the treadmill run.…”

Section: Recovery After Endurance Exercisementioning

confidence: 99%

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Myonectin deletion promotes adipose fat storage and reduces liver steatosis

et al. 2019

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We recently described myonectin (also known as erythroferrone) as a novel skeletal muscle‐derived myokine with metabolic functions. Here, we use a genetic mouse model to determine myonectin's requirement for metabolic homeostasis. Female myonectin‐deficient mice had larger gonadal fat pads and developed mild insulin resistance when fed a high‐fat diet (HFD) and had reduced food intake during refeeding after an unfed period but were otherwise indistinguishable from wild‐type littermates. Male mice lacking myonectin, however, had reduced physical activity when fed ad libitum and in the postprandial state but not during the unfed period. When stressed with an HFD, myonectin‐knockout male mice had significantly elevated VLDL‐triglyceride (TG) and strikingly impaired lipid clearance from circulation following an oral lipid load. Fat distribution between adipose and liver was also altered in myonectin‐deficient male mice fed an HFD. Greater fat storage resulted in significantly enlarged adipocytes and was associated with increased postprandial lipoprotein lipase activity in adipose tissue. Parallel to this was a striking reduction in liver steatosis due to significantly reduced TG accumulation. Liver metabolite profiling revealed additional significant changes in bile acids and 1‐carbon metabolism pathways. Combined, our data affirm the physiologic importance of myonectin in regulating local and systemic lipid metabolism.—Little, H. C., Rodriguez, S., Lei, X., Tan, S. Y., Stewart, A. N., Sahagun, A., Sarver, D. C., Wong, G. W. Myonectindeletion promotes adipose fat storage and reduces liver steatosis. FASEB J. 33, 8666–8687 (2019). http://www.fasebj.org

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Section: Maximal Exercise Capacitymentioning

confidence: 99%

Section: Recovery After Endurance Exercisementioning

confidence: 99%

Section: Recovery After Endurance Exercisementioning

confidence: 99%

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Myonectin deletion promotes adipose fat storage and reduces liver steatosis

et al. 2019

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“…Thus, secreted sCD30, sIL‐1RI, sIL‐1RII, sIL‐2Ra, sIL‐4R, sIL‐6R, sTNFRI, sTNFRII, sVEGFR1, sVEGFR2, sVEGFR3, sgp130, and sRAGE were also measured. We performed assays as previously described . Briefly, standard curves were generated for each mouse cytokine.…”

Section: Methodsmentioning

confidence: 99%

“…We performed assays as previously described. 70,71 Briefly, standard curves were generated for each mouse cytokine. Serum was diluted according to the manufacturer's instructions.…”

Section: Multiplex Quantification Of Serum Cytokinesmentioning

confidence: 99%

Late‐onset renal hypertrophy and dysfunction in mice lacking CTRP1

et al. 2019

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diameter at end of diastole; LVESD, LV chamber diameter at end of systole; PWTED, posterior wall thickness at end of diastole; PWV, pulse wave velocity; RWT, relative wall thickness; SV, stroke volume; WT, wild-type. AbstractLocal and systemic factors that influence renal structure and function in aging are not well understood. The secretory protein C1q/TNF-related protein 1 (CTRP1) regulates systemic metabolism and cardiovascular function. We provide evidence here that CTRP1 also modulates renal physiology in an age-and sex-dependent manner. In mice lacking CTRP1, we observed significantly increased kidney weight and glomerular hypertrophy in aged male but not female or young mice. Although glomerular filtration rate, plasma renin and aldosterone levels, and renal response to water restriction did not differ between genotypes, CTRP1-deficient male mice 2658 | RODRIGUEZ Et al.

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