Multiplex real-time PCR for the detection and differentiation of equid gammaherpesvirus 2 and 5

Fürer, Flavia; Fraefel, Cornel; Lechmann, Julia

doi:10.1016/j.jviromet.2022.114615

Cited by 3 publications

(1 citation statement)

References 48 publications

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“…Although the NSP3 target showed this lower sensitivity in the ERVA/ERVB VP6 quadruplex assay compared to the triplex assay previously developed, the G3 and G14 VP7 targets had comparable sensitivity. Reduced sensitivity of multiplex RT-qPCR or qPCR assays compared to singleplex counterparts is not unusual and has been previously reported [69][70][71][72][73][74][75][76][77]. Based on previous studies, the reduction in sensitivity compared to singleplex assays is typically slight and could be due to differential amplification of one target over others (based on the amplification efficiencies), target abundance, reagent competition, and non-specific interactions between primer sets or a combination of these [70][71][72]74].…”

Section: Discussionmentioning

confidence: 78%

Quadruplex Real-Time TaqMan® RT-qPCR Assay for Differentiation of Equine Group A and B Rotaviruses and Identification of Group A G3 and G14 Genotypes

Carossino

Balasuriya

Thieulent

et al. 2023

Viruses

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Equine rotavirus A (ERVA) is the leading cause of diarrhea in foals, with G3P[12] and G14P[12] genotypes being the most prevalent. Recently, equine G3-like RVA was recognized as an emerging infection in children, and a group B equine rotavirus (ERVB) was identified as an emergent cause of foal diarrhea in the US. Thus, there is a need to adapt molecular diagnostic tools for improved detection and surveillance to identify emerging strains, understand their molecular epidemiology, and inform future vaccine development. We developed a quadruplex TaqMan® RT-qPCR assay for differentiation of ERVA and ERVB and simultaneous G-typing of ERVA strains, evaluated its analytical and clinical performance, and compared it to (1) a previously established ERVA triplex RT-qPCR assay and (2) standard RT-PCR assay and Sanger sequencing of PCR products. This quadruplex RT-qPCR assay demonstrated high sensitivity (>90%)/specificity (100%) for every target and high overall agreement (>96%). Comparison between the triplex and quadruplex assays revealed only a slightly higher sensitivity for the ERVA NSP3 target using the triplex format (p-value 0.008) while no significant differences were detected for other targets. This quadruplex RT-qPCR assay will significantly enhance rapid surveillance of both ERVA and ERVB circulating and emerging strains with potential for interspecies transmission.

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Section: Discussionmentioning

confidence: 78%

Quadruplex Real-Time TaqMan® RT-qPCR Assay for Differentiation of Equine Group A and B Rotaviruses and Identification of Group A G3 and G14 Genotypes

Carossino

Balasuriya

Thieulent

et al. 2023

Viruses

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A triple lateral flow strip assay based on multiplex polymerase chain reaction for simultaneous detection of chicken, pork and duck in adulterated meat

Liu,

Lan,

et al. 2025

Journal of Food Composition and Analysis

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An Overview of Infectious and Non-Infectious Causes of Pregnancy Losses in Equine

Li,

et al. 2024

Animals

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Equine breeding plays an essential role in the local economic development of many countries, and it has experienced rapid growth in China in recent years. However, the equine industry, particularly large-scale donkey farms, faces a significant challenge with pregnancy losses. Unfortunately, there is a lack of systematic research on abortion during equine breeding. Several causes, both infectious and non-infectious, of pregnancy losses have been documented in equines. The infectious causes are viruses, bacteria, parasites, and fungi. Non-infectious causes may include long transportation, ingestion of mycotoxins, hormonal disturbances, twinning, placentitis, umbilical length and torsion, etc. In current review, we discuss the transmission routes, diagnostic methods, and control measures for these infectious agents. Early detection of the cause and appropriate management are crucial in preventing pregnancy loss in equine practice. This review aims to provide a comprehensive understanding of the potential causes of abortion in equines, including infectious agents and non-infectious factors. It emphasizes the importance of continued research and effective control measures to address this significant challenge in the equine industry.

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Multiplex real-time PCR for the detection and differentiation of equid gammaherpesvirus 2 and 5

Cited by 3 publications

References 48 publications

Quadruplex Real-Time TaqMan® RT-qPCR Assay for Differentiation of Equine Group A and B Rotaviruses and Identification of Group A G3 and G14 Genotypes

Quadruplex Real-Time TaqMan® RT-qPCR Assay for Differentiation of Equine Group A and B Rotaviruses and Identification of Group A G3 and G14 Genotypes

A triple lateral flow strip assay based on multiplex polymerase chain reaction for simultaneous detection of chicken, pork and duck in adulterated meat

An Overview of Infectious and Non-Infectious Causes of Pregnancy Losses in Equine

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